Piwil4 piwi-like RNA-mediated gene silencing 4 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Piwil4provided by MGI
Official Full Name: piwi-like RNA-mediated gene silencing 4provided by MGI
Primary source: MGI:MGI:3041167
See related: Ensembl:ENSMUSG00000036912 AllianceGenome:MGI:3041167
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Miwi2; mAgo5; 9230101H05Rik
Summary: Enables molecular adaptor activity and piRNA binding activity. Involved in negative regulation of gene expression and spermatogenesis. Acts upstream of with a positive effect on retrotransposon silencing by heterochromatin formation. Located in nucleus and piP-body. Is expressed in embryo; germ cell of testis; testis; and trunk unsegmented mesenchyme. Orthologous to human PIWIL4 (piwi like RNA-mediated gene silencing 4). [provided by Alliance of Genome Resources, Oct 2024]
Expression: Biased expression in genital fat pad adult (RPKM 2.0), colon adult (RPKM 1.2) and 10 other tissues See more
Orthologs: human all
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Genomic context

Location:: 9 A2; 9 4.25 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	9	NC_000075.7 (14613072..14651968, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	9	NC_000075.6 (14701776..14740672, complement)

Chromosome 9 - NC_000075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Two-factor authentication underpins the precision of the piRNA pathway.
Title: Two-factor authentication underpins the precision of the piRNA pathway.
The role of PIWIL4 and piRNAs in the development of choroidal neovascularization.
Title: The role of PIWIL4 and piRNAs in the development of choroidal neovascularization.
TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing.
Title: TEX15 is an essential executor of MIWI2-directed transposon DNA methylation and silencing.
Somatic MIWI2 hinders direct lineage reprogramming from fibroblasts to hepatocytes.
Title: Somatic MIWI2 Hinders Direct Lineage Reprogramming From Fibroblast to Hepatocyte.
results indicate that MIWI2 affects the chromatin state through base-pairing between piRNAs and nascent RNAs, as observed in other organisms possessing small RNA-mediated epigenetic regulation.
Title: MIWI2 targets RNAs transcribed from piRNA-dependent regions to drive DNA methylation in mouse prospermatogonia.
MIWI2 functions as an effector of de novo DNA methylation of the retrotransposon.
Title: MIWI2 as an Effector of DNA Methylation and Gene Silencing in Embryonic Male Germ Cells.
data delineate MIWI2-dependent functions outside of the germline and demonstrate the presence of distinct subsets of airway multiciliated cells that can be discriminated by MIWI2 expression.
Title: Expression of Piwi protein MIWI2 defines a distinct population of multiciliated cells.
Miwi2 deficiency had only a minor impact on piRNA biogenesis. Miwi2-knockout mice indicated overexpression of several LINE1 TE families.
Title: MIWI2 and MILI Have Differential Effects on piRNA Biogenesis and DNA Methylation.
piRNA biogenesis triggered by PIWI slicing, and promoted by EXD1, ensures that the same guides instruct PIWI proteins in the nucleus and cytoplasm.
Title: PIWI Slicing and EXD1 Drive Biogenesis of Nuclear piRNAs from Cytosolic Targets of the Mouse piRNA Pathway.
MIWI2 is not solely necessary for hematopoiesis within the normal life span of a mouse.
Title: Deficiency of MIWI2 (Piwil4) induces mouse erythroleukemia cell differentiation, but has no effect on hematopoiesis in vivo.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2) 1 citation
Targeted (9) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
NOT enables RNA endonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables molecular adaptor activity	IDA Inferred from Direct Assay more info	PubMed
enables piRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables piRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables piRNA binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in epithelial structure maintenance	ISO Inferred from Sequence Orthology more info
involved_in epithelial structure maintenance	ISS Inferred from Sequence or Structural Similarity more info
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in piRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in piRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in piRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in piRNA-mediated gene silencing by mRNA destabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of insulin secretion involved in cellular response to glucose stimulus	ISO Inferred from Sequence Orthology more info
involved_in regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in regulatory ncRNA-mediated gene silencing	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within retrotransposon silencing by heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_positive_effect retrotransposon silencing by heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retrotransposon silencing by mRNA destabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retrotransposon silencing by piRNA-directed DNA methylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retrotransposon silencing by piRNA-directed DNA methylation	IPI Inferred from Physical Interaction more info	PubMed
involved_in secondary piRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in secondary piRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in P granule	IBA Inferred from Biological aspect of Ancestor more info
located_in P granule	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in piP-body	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: piwi-like protein 4

Names: piwi-like 4; piwi-like homolog 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001368831.1 → NP_001355760.1 piwi-like protein 4 isoform 1

Status: VALIDATED

Source sequence(s)

CT030247

Consensus CDS

CCDS22824.2

UniProtKB/Swiss-Prot

A6P4B9, A6X963, A6X964, Q8CC75, Q8CGT6

UniProtKB/TrEMBL

A0A6Q6QBZ6

Related

ENSMUSP00000111308.4, ENSMUST00000115644.10

Conserved Domains (2) summary

smart00949
Location:266 → 403

PAZ; This domain is named PAZ after the proteins Piwi Argonaut and Zwille

cd04658
Location:389 → 831

Piwi_piwi-like_Euk; PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of related ...
NM_001368832.1 → NP_001355761.1 piwi-like protein 4 isoform 2

Status: VALIDATED

Source sequence(s)

CT030247

UniProtKB/TrEMBL

A0A6Q6QBZ6

Conserved Domains (2) summary

smart00949
Location:266 → 403

PAZ; This domain is named PAZ after the proteins Piwi Argonaut and Zwille

cd04658
Location:389 → 830

Piwi_piwi-like_Euk; PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of related ...
NM_001368836.1 → NP_001355765.1 piwi-like protein 4 isoform 3

Status: VALIDATED

Source sequence(s)

CT030247

Related

ENSMUST00000115643.8

Conserved Domains (1) summary

cd04658
Location:1 → 441

Piwi_piwi-like_Euk; PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of related ...

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000075.7 Reference GRCm39 C57BL/6J

Range

14613072..14651968 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006510433.4 → XP_006510496.1 piwi-like protein 4 isoform X1

Conserved Domains (2) summary

smart00949
Location:27 → 130

PAZ; This domain is named PAZ after the proteins Piwi Argonaut and Zwille

cd04658
Location:116 → 558

Piwi_piwi-like_Euk; PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of related ...
XM_036155071.1 → XP_036010964.1 piwi-like protein 4 isoform X3

Conserved Domains (1) summary

cd04658
Location:7 → 419

Piwi_piwi-like_Euk; PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of related ...
XM_036155070.1 → XP_036010963.1 piwi-like protein 4 isoform X2

Conserved Domains (2) summary

smart00949
Location:53 → 190

PAZ; This domain is named PAZ after the proteins Piwi Argonaut and Zwille

cl00628
Location:176 → 455

Piwi-like; PIWI domain. Domain found in proteins involved in RNA silencing. RNA silencing refers to a group of related gene-silencing mechanisms mediated by short RNA molecules, including siRNAs, miRNAs, and heterochromatin-related guide RNAs. The central component ...

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_177905.3: Suppressed sequence

Description

NM_177905.3: This RefSeq was removed because currently there is insufficient support for the transcript and the protein.