Chrnb4 cholinergic receptor nicotinic beta 4 subunit [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Chrnb4provided by RGD
Official Full Name: cholinergic receptor nicotinic beta 4 subunitprovided by RGD
Primary source: RGD:2351
See related: EnsemblRapid:ENSRNOG00000014427 AllianceGenome:RGD:2351
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables acetylcholine binding activity and heterocyclic compound binding activity. Predicted to be involved in several processes, including membrane depolarization; positive regulation of transmission of nerve impulse; and synaptic signaling. Predicted to act upstream of or within several processes, including behavioral response to nicotine; neuronal action potential; and synaptic transmission involved in micturition. Located in plasma membrane. Part of acetylcholine-gated channel complex. Biomarker of depressive disorder. Human ortholog(s) of this gene implicated in cocaine abuse; lung cancer (multiple); nicotine dependence; and oral cavity cancer. Orthologous to human CHRNB4 (cholinergic receptor nicotinic beta 4 subunit). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Adrenal (RPKM 19.8), Thymus (RPKM 5.8) and 2 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 8q24

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (64312644..64333319, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (55417583..55437027, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (59610489..59629073, complement)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Results suggest the fast nAChR current in cutaneous dorsal root ganglia neurons was carried by homomeric alpha7 nAChRs while the slow current was carried by heteromeric (most likely alpha3beta4) nAChRs
Title: Inflammation-induced increase in nicotinic acetylcholine receptor current in cutaneous nociceptive DRG neurons from the adult rat.
Four genes were significantly downregulated in the PFC [prefrontal cortex ]of SHR[spontaneously hypertensive rat ] compared to Wistar rats (Gad2, Chrnb4, Slc5a7, and Qrfpr) and none in nucleus accumbens.
Title: Expression profile of neurotransmitter receptor and regulatory genes in the prefrontal cortex of spontaneously hypertensive rats: relevance to neuropsychiatric disorders.
alpha6beta4* nAChRs are among the subtypes expressed by dorsal root ganglion neurons, and to our knowledge, this is the first demonstration of alpha6beta4* in neurons outside the CNS
Title: Nicotinic acetylcholine receptors in dorsal root ganglion neurons include the α6β4* subtype.
The role of negatively charged amino acids in the F-loop of the beta 4 subunit in channel activation and desensitization was studied using the patch-clamp technique.
Title: Role of negatively charged amino acids in beta 4 F-loop in activation and desensitization of alpha 3 beta 4 rat neuronal nicotinic receptors.
Loop 9 has a different role in the function of homomeric and heteromeric receptors.
Title: Role of loop 9 on the function of neuronal nicotinic receptors.
Immunocytochemistry revealed most chromaffin cells positive for beta4 proteins. And suggest that the most frequently encountered receptors of rat chromaffin cells should comprise alpha3beta4, alpha3beta2 with the addition of alpha5 subunits.
Title: Molecular biology and electrophysiology of neuronal nicotinic receptors of rat chromaffin cells.
Only residues belonging to two beta2 segments, 74-89 and 106-115, confer up-regulation to beta4, mainly by decreasing the amount of binding sites in the absence of nicotine; the beta4 microdomain is sufficient to confer to beta2 a beta4-like up-regulation
Title: An extracellular protein microdomain controls up-regulation of neuronal nicotinic acetylcholine receptors by nicotine.

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General gene information

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Markers

Chrnb4 (e-PCR), detects polymorphism
- UniSTS:261122

PMC312758P4 (e-PCR)
- UniSTS:272915

Chrnb4 (e-PCR), detects polymorphism
- UniSTS:256428

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables acetylcholine binding	IDA Inferred from Direct Assay more info	PubMed
enables acetylcholine receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables acetylcholine receptor activity	ISO Inferred from Sequence Orthology more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables acetylcholine-gated monoatomic cation-selective channel activity	IC Inferred by Curator more info	PubMed
enables acetylcholine-gated monoatomic cation-selective channel activity	IEA Inferred from Electronic Annotation more info
enables acetylcholine-gated monoatomic cation-selective channel activity	ISO Inferred from Sequence Orthology more info
enables heterocyclic compound binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to heterocyclic compound binding	IPI Inferred from Physical Interaction more info	PubMed
enables heterocyclic compound binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed
enables transmembrane signaling receptor activity	IEA Inferred from Electronic Annotation more info
enables transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in acetylcholine receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within behavioral response to nicotine	ISO Inferred from Sequence Orthology more info
involved_in chemical synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in excitatory postsynaptic potential	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within locomotory behavior	ISO Inferred from Sequence Orthology more info
involved_in membrane depolarization	IBA Inferred from Biological aspect of Ancestor more info
involved_in monoatomic ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in monoatomic ion transmembrane transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within neuronal action potential	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transmission of nerve impulse	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of membrane potential	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of smooth muscle contraction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to nicotine	ISO Inferred from Sequence Orthology more info
involved_in signal transduction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within smooth muscle contraction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within synaptic transmission involved in micturition	ISO Inferred from Sequence Orthology more info
involved_in synaptic transmission involved in micturition	ISO Inferred from Sequence Orthology more info
involved_in synaptic transmission, cholinergic	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of acetylcholine-gated channel complex	IBA Inferred from Biological aspect of Ancestor more info
part_of acetylcholine-gated channel complex	IDA Inferred from Direct Assay more info	PubMed
part_of acetylcholine-gated channel complex	ISO Inferred from Sequence Orthology more info
part_of acetylcholine-gated channel complex	TAS Traceable Author Statement more info	PubMed
is_active_in cholinergic synapse	ISO Inferred from Sequence Orthology more info
is_active_in neuron projection	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in postsynaptic membrane	IEA Inferred from Electronic Annotation more info
is_active_in postsynaptic specialization membrane	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: neuronal acetylcholine receptor subunit beta-4

Names: Acetylcholine receptor beta 4; N-alpha 2; cholinergic receptor, nicotinic beta 4; cholinergic receptor, nicotinic, beta 4 (neuronal); cholinergic receptor, nicotinic, beta polypeptide 4; neuronal acetylcholine receptor non-alpha-2 chain; nicotinic acetylcholine receptor-related protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_052806.3 → NP_434693.1 neuronal acetylcholine receptor subunit beta-4 precursor

See identical proteins and their annotated locations for NP_434693.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000008

UniProtKB/Swiss-Prot

P12392, Q63361

UniProtKB/TrEMBL

A0A8I5Y7T1, A6J4N9

Related

ENSRNOP00000019458.2, ENSRNOT00000019458.7

Conserved Domains (2) summary

TIGR00860
Location:21 → 483

LIC; Cation transporter family protein

cl28912
Location:53 → 230

LGIC_ECD; extracellular domain (ECD) of Cys-loop neurotransmitter-gated ion channels (also known as ligand-gated ion channel (LGIC))

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086026.1 Reference GRCr8

Range

64312644..64333319 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063264942.1 → XP_063121012.1 neuronal acetylcholine receptor subunit beta-4 isoform X1
XM_063264943.1 → XP_063121013.1 neuronal acetylcholine receptor subunit beta-4 isoform X2