Mid2 midline 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mid2provided by MGI
Official Full Name: midline 2provided by MGI
Primary source: MGI:MGI:1344333
See related: Ensembl:ENSMUSG00000000266 AllianceGenome:MGI:1344333
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: FXY2; Trim1
Summary: Predicted to enable several functions, including microtubule binding activity; protein homodimerization activity; and transcription coactivator activity. Predicted to be involved in several processes, including defense response to symbiont; negative regulation of viral transcription; and positive regulation of NF-kappaB transcription factor activity. Predicted to be located in cytoplasm. Predicted to colocalize with microtubule. Is expressed in several structures, including alimentary system; branchial arch; central nervous system; heart; and sensory organ. Human ortholog(s) of this gene implicated in non-syndromic X-linked intellectual disability 101. Orthologous to human MID2 (midline 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in lung adult (RPKM 6.4), bladder adult (RPKM 4.0) and 26 other tissues See more
Orthologs: human all
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Genomic context

Location:: X F1; X 61.35 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	X	NC_000086.8 (139565326..139668464)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	X	NC_000086.7 (140664304..140767715)

Chromosome X - NC_000086.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

both MID1 and MID2 play a central role in the regulation of granule exocytosis and that functional redundancy exists between MID1 and MID2 in cytotoxic T cells
Title: MID2 can substitute for MID1 and control exocytosis of lytic granules in cytotoxic T cells.

Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Mid2 (e-PCR), detects polymorphism
- UniSTS:546866

Mid2 (e-PCR), detects polymorphism
- UniSTS:143869

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule binding	ISO Inferred from Sequence Orthology more info
enables phosphoprotein binding	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables transcription coactivator activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in innate immune response	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of viral entry into host cell	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of viral transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of autophagy	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of autophagy	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in protein localization to microtubule	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein localization to microtubule	ISO Inferred from Sequence Orthology more info
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in suppression of viral release by host	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
colocalizes_with microtubule	IBA Inferred from Biological aspect of Ancestor more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
colocalizes_with microtubule	ISO Inferred from Sequence Orthology more info
located_in microtubule	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: probable E3 ubiquitin-protein ligase MID2

Names: RING-type E3 ubiquitin transferase MID2; midline defect 2; tripartite motif-containing protein 1

NP_001345295.1

EC 2.3.2.27

NP_001345296.1

EC 2.3.2.27

NP_001390295.1

EC 2.3.2.27

NP_035975.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001358366.2 → NP_001345295.1 probable E3 ubiquitin-protein ligase MID2 isoform 2

Status: VALIDATED

Source sequence(s)

AL683809, BX470203

UniProtKB/Swiss-Prot

Q9QUS6

UniProtKB/TrEMBL

B1AVF5

Related

ENSMUSP00000108614.2, ENSMUST00000112990.8

Conserved Domains (5) summary

smart00502
Location:239 → 364

BBC; B-Box C-terminal domain

smart00336
Location:195 → 232

BBOX; B-Box-type zinc finger

cd00063
Location:401 → 501

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd13739
Location:506 → 675

SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2

cd16754
Location:30 → 82

RING-HC_MID2; RING finger, HC subclass, found in midline-2 (MID2) and similar proteins
NM_001358367.2 → NP_001345296.1 probable E3 ubiquitin-protein ligase MID2 isoform 1

Status: VALIDATED

Source sequence(s)

AL683809, BX470203

UniProtKB/TrEMBL

B1AVF5

Related

ENSMUSP00000108612.2, ENSMUST00000112988.8

Conserved Domains (5) summary

smart00502
Location:239 → 364

BBC; B-Box C-terminal domain

smart00336
Location:195 → 232

BBOX; B-Box-type zinc finger

cd00063
Location:401 → 531

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd13739
Location:536 → 705

SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2

cd16754
Location:30 → 82

RING-HC_MID2; RING finger, HC subclass, found in midline-2 (MID2) and similar proteins
NM_001403366.1 → NP_001390295.1 probable E3 ubiquitin-protein ligase MID2 isoform 4

Status: VALIDATED

Source sequence(s)

AL683809, BX470203
NM_011845.4 → NP_035975.1 probable E3 ubiquitin-protein ligase MID2 isoform 3

See identical proteins and their annotated locations for NP_035975.1

Status: VALIDATED

Source sequence(s)

AL683809, BX470203

Consensus CDS

CCDS41151.1

UniProtKB/TrEMBL

B1AVF4, B1AVF5

Related

ENSMUSP00000108617.2, ENSMUST00000112993.2

Conserved Domains (7) summary

smart00502
Location:219 → 344

BBC; B-Box C-terminal domain

cd00063
Location:381 → 481

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

cd13739
Location:486 → 655

SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2

cd16754
Location:10 → 62

RING-HC_MID2; RING finger, HC subclass, found in midline-2 (MID2) and similar proteins

pfam18568
Location:323 → 374

COS; TRIM C-terminal subgroup One Signature domain

cd19823
Location:173 → 212

Bbox2_MID2_C-I; B-box-type 2 zinc finger found in midline-2 (MID2) and similar proteins

cd19837
Location:115 → 167

Bbox1_MID2_C-I; B-box-type 1 zinc finger found in midline-2 (MID2) and similar proteins