Rock2 Rho-associated coiled-coil containing protein kinase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rock2provided by MGI
Official Full Name: Rho-associated coiled-coil containing protein kinase 2provided by MGI
Primary source: MGI:MGI:107926
See related: Ensembl:ENSMUSG00000020580 AllianceGenome:MGI:107926
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Rock2m; Rock-II; ROKalpha; mKIAA0619; Rho-kinase; B230113H15Rik
Summary: Enables protein serine/threonine kinase activity. Involved in several processes, including negative regulation of nitric oxide biosynthetic process; positive regulation of macromolecule metabolic process; and regulation of signal transduction. Acts upstream of or within several processes, including extrinsic apoptotic signaling pathway via death domain receptors; nervous system development; and positive regulation of centrosome duplication. Located in spindle pole centrosome. Is active in several cellular components, including Schaffer collateral - CA1 synapse; glutamatergic synapse; and postsynaptic density. Is expressed in several structures, including central nervous system; genitourinary system; heart; musculature; and somite. Orthologous to human ROCK2 (Rho associated coiled-coil containing protein kinase 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in bladder adult (RPKM 16.5), cortex adult (RPKM 10.8) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 12 A1.1; 12 8.04 cM

Exon count:: 37

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	12	NC_000078.7 (16944808..17038275)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	12	NC_000078.6 (16894978..16988274)

Chromosome 12 - NC_000078.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

miR-101a-3p/ROCK2 axis regulates neuronal injury in Parkinson's disease models.
Title: miR-101a-3p/ROCK2 axis regulates neuronal injury in Parkinson's disease models.
ROCK1/2 signaling contributes to corticosteroid-refractory acute graft-versus-host disease.
Title: ROCK1/2 signaling contributes to corticosteroid-refractory acute graft-versus-host disease.
Deletion of podocyte Rho-associated, coiled-coil-containing protein kinase 2 protects mice from focal segmental glomerulosclerosis.
Title: Deletion of podocyte Rho-associated, coiled-coil-containing protein kinase 2 protects mice from focal segmental glomerulosclerosis.
Inhibition of microRNA-34c reduces detrusor ROCK2 expression and urinary bladder inflammation in experimental cystitis.
Title: Inhibition of microRNA-34c reduces detrusor ROCK2 expression and urinary bladder inflammation in experimental cystitis.
A 3D biomimetic model of lymphatics reveals cell-cell junction tightening and lymphedema via a cytokine-induced ROCK2/JAM-A complex.
Title: A 3D biomimetic model of lymphatics reveals cell-cell junction tightening and lymphedema via a cytokine-induced ROCK2/JAM-A complex.
[Knock-down of ROCK2 gene improves cognitive function and reduces neuronal apoptosis in AD mice by promoting mitochondrial fusion and inhibiting its division].
Title: [Knock-down of ROCK2 gene improves cognitive function and reduces neuronal apoptosis in AD mice by promoting mitochondrial fusion and inhibiting its division].
alpha-Actinin-4 recruits Shp2 into focal adhesions to potentiate ROCK2 activation in podocytes.
Title: α-Actinin-4 recruits Shp2 into focal adhesions to potentiate ROCK2 activation in podocytes.
FOXO4 alleviates hippocampal neuronal damage in epileptic mice via the miR-138-5p/ROCK2 axis.
Title: FOXO4 alleviates hippocampal neuronal damage in epileptic mice via the miR-138-5p/ROCK2 axis.
Activity of ROCKII not ROCKI promotes pulmonary metastasis of melanoma cells via modulating Smad2/3-MMP9 and FAK-Src-VEGF signalling.
Title: Activity of ROCKII not ROCKI promotes pulmonary metastasis of melanoma cells via modulating Smad2/3-MMP9 and FAK-Src-VEGF signalling.
Conditional deletion of ROCK2 induces anxiety-like behaviors and alters dendritic spine density and morphology on CA1 pyramidal neurons.
Title: Conditional deletion of ROCK2 induces anxiety-like behaviors and alters dendritic spine density and morphology on CA1 pyramidal neurons.

Submit: New GeneRIF Correction See all GeneRIFs (133)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Gene trapped (1) 1 citation
Targeted (14) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:236811

Rock2 (e-PCR), detects polymorphism
- UniSTS:516658

AW823633 (e-PCR)
- UniSTS:208783

RH126996 (e-PCR)
- UniSTS:208785

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables Rho-dependent protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables endopeptidase activator activity	ISO Inferred from Sequence Orthology more info
enables endopeptidase activator activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protease binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables small GTPase binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Rho protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within Rho protein signal transduction	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within actin cytoskeleton organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in actin cytoskeleton organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in actomyosin structure organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in blood vessel diameter maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in cellular response to acetylcholine	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to testosterone stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of centrosome duplication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centrosome duplication	ISO Inferred from Sequence Orthology more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in cortical actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in cortical actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within dendrite morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in embryonic morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in epithelial to mesenchymal transition	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within extrinsic apoptotic signaling pathway via death domain receptors	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mRNA destabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic cytokinesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in modulation by host of viral process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of bicellular tight junction assembly	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of nitric oxide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of nitric oxide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein localization to lysosome	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neural tube closure	IGI Inferred from Genetic Interaction more info	PubMed
involved_in peptidyl-threonine phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of amyloid precursor protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of amyloid precursor protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of amyloid-beta formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of amyloid-beta formation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cardiac muscle hypertrophy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cardiac muscle hypertrophy	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of centrosome duplication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of connective tissue growth factor production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of connective tissue replacement	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of connective tissue replacement	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of endothelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of fibroblast growth factor production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein localization to early endosome	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of stress fiber assembly	ISO Inferred from Sequence Orthology more info
involved_in postsynaptic actin cytoskeleton organization	IDA Inferred from Direct Assay more info	PubMed
involved_in postsynaptic actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of actin cytoskeleton organization	IEA Inferred from Electronic Annotation more info
involved_in regulation of angiotensin-activated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell junction assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of cellular response to hypoxia	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of circadian rhythm	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of establishment of endothelial barrier	ISO Inferred from Sequence Orthology more info
involved_in regulation of keratinocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in regulation of nervous system process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of synapse maturation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of synapse maturation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to angiotensin	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to ischemia	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to transforming growth factor beta	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to transforming growth factor beta	ISO Inferred from Sequence Orthology more info
involved_in rhythmic process	IEA Inferred from Electronic Annotation more info
involved_in smooth muscle contraction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in Schaffer collateral - CA1 synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in Schaffer collateral - CA1 synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in centrosome	IBA Inferred from Biological aspect of Ancestor more info
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic ribonucleoprotein granule	ISO Inferred from Sequence Orthology more info
is_active_in cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
is_active_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynaptic active zone	IDA Inferred from Direct Assay more info	PubMed
located_in spindle pole centrosome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: rho-associated protein kinase 2

Names: Rho-associated coiled-coil forming kinage 2; p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase 2; rho-associated, coiled-coil-containing protein kinase II

NP_033098.2

EC 2.7.11.1

XP_036013163.1

EC 2.7.11.1

XP_036013164.1

EC 2.7.11.1

XP_036013165.1

EC 2.7.11.1

XP_036013166.1

EC 2.7.11.1

XP_036013167.1

EC 2.7.11.1

XP_036013168.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_009072.2 → NP_033098.2 rho-associated protein kinase 2

See identical proteins and their annotated locations for NP_033098.2

Status: VALIDATED

Source sequence(s)

AK045517, AK048955, AK153280, AK163082, CJ127012, CN536850, U58513

Consensus CDS

CCDS36410.1

UniProtKB/Swiss-Prot

A5XDA7, P70336, Q8BR64, Q8CBR0

UniProtKB/TrEMBL

F8VPK5

Related

ENSMUSP00000020904.7, ENSMUST00000020904.8

Conserved Domains (7) summary

cd11638
Location:505 → 571

HR1_ROCK2; Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Rho-associated coiled-coil containing protein kinase 2

smart00220
Location:92 → 354

S_TKc; Serine/Threonine protein kinases, catalytic domain

COG1196
Location:437 → 1101

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd00029
Location:1261 → 1307

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01242
Location:1151 → 1257

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05621
Location:39 → 417

STKc_ROCK2; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase 2

pfam08912
Location:979 → 1046

Rho_Binding; Rho Binding

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000078.7 Reference GRCm39 C57BL/6J

Range

16944808..17038275

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036157270.1 → XP_036013163.1 rho-associated protein kinase 2 isoform X1

UniProtKB/TrEMBL

A0A1Y7VMN0

Conserved Domains (5) summary

COG1196
Location:437 → 1101

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd01242
Location:1208 → 1314

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05621
Location:39 → 417

STKc_ROCK2; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase 2

cd22250
Location:856 → 936

ROCK_SBD; Shroom-binding domain found in Rho-associated coiled-coil containing protein kinase

cd20875
Location:1307 → 1377

C1_ROCK2; protein kinase C conserved region 1 (C1 domain) found in Rho-associated coiled-coil containing protein kinase 2 (ROCK2) and similar proteins
XM_036157271.1 → XP_036013164.1 rho-associated protein kinase 2 isoform X2

UniProtKB/TrEMBL

A0A1Y7VMN0

Related

ENSMUSP00000152813.2, ENSMUST00000220688.2

Conserved Domains (5) summary

COG1196
Location:437 → 1101

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd01242
Location:1208 → 1314

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05621
Location:39 → 417

STKc_ROCK2; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase 2

cd22250
Location:856 → 936

ROCK_SBD; Shroom-binding domain found in Rho-associated coiled-coil containing protein kinase

cd20875
Location:1307 → 1377

C1_ROCK2; protein kinase C conserved region 1 (C1 domain) found in Rho-associated coiled-coil containing protein kinase 2 (ROCK2) and similar proteins
XM_036157272.1 → XP_036013165.1 rho-associated protein kinase 2 isoform X3

UniProtKB/Swiss-Prot

A5XDA7, P70336, Q8BR64, Q8CBR0

Conserved Domains (5) summary

COG1196
Location:437 → 1101

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd01242
Location:1151 → 1257

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05621
Location:39 → 417

STKc_ROCK2; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase 2

cd22250
Location:856 → 936

ROCK_SBD; Shroom-binding domain found in Rho-associated coiled-coil containing protein kinase

cd20875
Location:1250 → 1320

C1_ROCK2; protein kinase C conserved region 1 (C1 domain) found in Rho-associated coiled-coil containing protein kinase 2 (ROCK2) and similar proteins
XM_036157273.1 → XP_036013166.1 rho-associated protein kinase 2 isoform X4

UniProtKB/TrEMBL

A0A1Y7VMN0

Conserved Domains (5) summary

COG1196
Location:318 → 982

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd01242
Location:1089 → 1195

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05596
Location:1 → 296

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

cd22250
Location:737 → 817

ROCK_SBD; Shroom-binding domain found in Rho-associated coiled-coil containing protein kinase

cd20875
Location:1188 → 1258

C1_ROCK2; protein kinase C conserved region 1 (C1 domain) found in Rho-associated coiled-coil containing protein kinase 2 (ROCK2) and similar proteins
XM_036157274.1 → XP_036013167.1 rho-associated protein kinase 2 isoform X4

UniProtKB/TrEMBL

A0A1Y7VMN0

Conserved Domains (5) summary

COG1196
Location:318 → 982

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd01242
Location:1089 → 1195

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05596
Location:1 → 296

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

cd22250
Location:737 → 817

ROCK_SBD; Shroom-binding domain found in Rho-associated coiled-coil containing protein kinase

cd20875
Location:1188 → 1258

C1_ROCK2; protein kinase C conserved region 1 (C1 domain) found in Rho-associated coiled-coil containing protein kinase 2 (ROCK2) and similar proteins
XM_036157275.1 → XP_036013168.1 rho-associated protein kinase 2 isoform X4

UniProtKB/TrEMBL

A0A1Y7VMN0

Conserved Domains (5) summary

COG1196
Location:318 → 982

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd01242
Location:1089 → 1195

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05596
Location:1 → 296

STKc_ROCK; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase

cd22250
Location:737 → 817

ROCK_SBD; Shroom-binding domain found in Rho-associated coiled-coil containing protein kinase

cd20875
Location:1188 → 1258

C1_ROCK2; protein kinase C conserved region 1 (C1 domain) found in Rho-associated coiled-coil containing protein kinase 2 (ROCK2) and similar proteins