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    Pknox1 Pbx/knotted 1 homeobox [ Mus musculus (house mouse) ]

    Gene ID: 18771, updated on 14-Nov-2024

    Summary

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    Official Symbol
    Pknox1provided by MGI
    Official Full Name
    Pbx/knotted 1 homeoboxprovided by MGI
    Primary source
    MGI:MGI:1201409
    See related
    Ensembl:ENSMUSG00000006705 AllianceGenome:MGI:1201409
    Gene type
    protein coding
    RefSeq status
    VALIDATED
    Organism
    Mus musculus
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
    Also known as
    PREP1; D17Wsu76e
    Summary
    Enables DNA-binding transcription factor activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and chromatin binding activity. Acts upstream of or within several processes, including angiogenesis; hemopoiesis; and positive regulation of transcription by RNA polymerase II. Located in cytoplasm and nucleus. Part of transcription regulator complex. Is expressed in several structures, including alimentary system; branchial arch; genitourinary system; nervous system; and sensory organ. Orthologous to human PKNOX1 (PBX/knotted 1 homeobox 1). [provided by Alliance of Genome Resources, Nov 2024]
    Expression
    Ubiquitous expression in thymus adult (RPKM 12.1), testis adult (RPKM 10.4) and 28 other tissues See more
    Orthologs
    human all
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    Genomic context

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    See Pknox1 in Genome Data Viewer
    Location:
    17 B1; 17 16.78 cM
    Exon count:
    12
    Annotation release Status Assembly Chr Location
    RS_2024_02 current GRCm39 (GCF_000001635.27) 17 NC_000083.7 (31783697..31826665)
    108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 17 NC_000083.6 (31564722..31607693)

    Chromosome 17 - NC_000083.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E3197 Neighboring gene NADH:ubiquinone oxidoreductase core subunit V3 Neighboring gene predicted gene, 50105 Neighboring gene STARR-positive B cell enhancer ABC_E9439 Neighboring gene STARR-seq mESC enhancer starr_42341 Neighboring gene STARR-seq mESC enhancer starr_42343 Neighboring gene cystathionine beta-synthase Neighboring gene STARR-seq mESC enhancer starr_42345 Neighboring gene ribosomal protein S2, pseudogene 8

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
    • Description: RNA profiling data sets generated by the Mouse ENCODE project.
    • BioProject: PRJNA66167
    • Publication: PMID 25409824
    • Analysis date: n/a

    Bibliography

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    Related articles in PubMed

    1. Interleukin 6 reduces vascular smooth muscle cell apoptosis via Prep1 and is associated with aging. Cimmino I, et al. FASEB J, 2021 Nov. PMID 34679197
    2. Pknox1/Prep1 regulates mitochondrial oxidative phosphorylation components in skeletal muscle. Kanzleiter T, et al. Mol Cell Biol, 2014 Jan. PMID 24216763, Free PMC Article
    3. Prep1 (pKnox1) regulates mouse embryonic HSC cycling and self-renewal affecting the Stat1-Sca1 IFN-dependent pathway. Modica L, et al. PLoS One, 2014. PMID 25233378, Free PMC Article
    4. ChIP-Seq and RNA-Seq analyses identify components of the Wnt and Fgf signaling pathways as Prep1 target genes in mouse embryonic stem cells. Laurent A, et al. PLoS One, 2015. PMID 25875616, Free PMC Article
    5. Loss of the Homeodomain Transcription Factor Prep1 Perturbs Adult Hematopoiesis in the Bone Marrow. Yoshioka K, et al. PLoS One, 2015. PMID 26285139, Free PMC Article

    See all (100) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Interleukin 6 reduces vascular smooth muscle cell apoptosis via Prep1 and is associated with aging.
      Title: Interleukin 6 reduces vascular smooth muscle cell apoptosis via Prep1 and is associated with aging.
    2. Prep1, complexing with p160, decreases PGC-1alpha and stimulates angiogenesis.
      Title: Prep1 regulates angiogenesis through a PGC-1α-mediated mechanism.
    3. Prep1 DR expands the DNA binding landscape of C/EBPb (CCAAT enhancer binding protein b) without affecting its expression or activation. The data indicate that Prep1 normally acts by restricting DNA binding of transcription factors to adipogenic enhancers, in particular C/EBPb.
      Title: Prep1 prevents premature adipogenesis of mesenchymal progenitors.
    4. We conclude that Prep1 is required for normal adult mammary gland development, in particular at its branching morphogenesis step. By binding Snail2, Prep1 protects it from the proteasomal degradation.
      Title: Prep1 (pKnox1) transcription factor contributes to pubertal mammary gland branching morphogenesis.
    5. a mechanism by which the DNA binding-independent activity of Sp2 potentiates genomic loading of Pbx1:Prep1 and Nf-y to composite motifs present in many promoters of highly expressed genes
      Title: Transcription factor Sp2 potentiates binding of the TALE homeoproteins Pbx1:Prep1 and the histone-fold domain protein Nf-y to composite genomic sites.
    6. Prep1 deficiency alters olfactory morpho-functional integrity and olfaction-mediated eating behavior by affecting BDNF-TrkB signaling.
      Title: Prep1 Deficiency Affects Olfactory Perception and Feeding Behavior by Impairing BDNF-TrkB Mediated Neurotrophic Signaling.
    7. Prep1 silencing reduces inflammatory response and increases insulin sensitivity in adipose tissue.
      Title: Prep1 deficiency improves metabolic response in white adipose tissue.
    8. results indicate that PKnox1 is a critical regulator of maintenance and subsequent differentiation of the c-Kit+ stage of spermatogonia in the adult testes
      Title: Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis.
    9. However, Prep1 doesn't affect gluconeogenesis in the absence of insulin. Also, we observed that nuclear content of gluconeogenic transcription factor FOXO1 was greatly reduced in Prep1 knockout hepatocytes. These findings suggest that Prep1 may control hepatic insulin sensitivity by targeting FOXO1 nuclear stability.
      Title: The transcription factor Prep1 controls hepatic insulin sensitivity and gluconeogenesis by targeting nuclear localization of FOXO1.
    10. Study found that knockdown of Prep1 gene expression affects adipogenic differentiation and results in significant increase in the insulin-sensitive glucose carrier Glut4 gene expression
      Title: Transcriptional control of insulin-sensitive glucose carrier Glut4 expression in adipose tissue cells.
    Submit: New GeneRIF Correction See all GeneRIFs (36)

    Variation

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    Alleles

    Alleles of this type are documented at Mouse Genome Informatics  (MGI)
    • Endonuclease-mediated (1) 
    • Gene trapped (4) 
    • Targeted (7)  1 citation

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by MGI

    Function Evidence Code Pubs
    enables DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA-binding transcription factor activity, RNA polymerase II-specific IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    enables chromatin binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables sequence-specific double-stranded DNA binding ISO
    Inferred from Sequence Orthology
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within T cell differentiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in angiogenesis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within angiogenesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within camera-type eye development IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within erythrocyte differentiation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within hemopoiesis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of DNA-templated transcription TAS
    Traceable Author Statement
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of transcription by RNA polymerase II TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of transcription regulator complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    homeobox protein PKNOX1
    Names
    PBX/knotted homeobox 1

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001411456.1 → NP_001398385.1  homeobox protein PKNOX1 isoform 1

      Status: VALIDATED

      Source sequence(s)
      AC166172, AC166575
      UniProtKB/Swiss-Prot
      O70477, Q7TT01

    2. NM_001411457.1 → NP_001398386.1  homeobox protein PKNOX1 isoform 1

      Status: VALIDATED

      Source sequence(s)
      AC166172, AC166575
      UniProtKB/Swiss-Prot
      O70477, Q7TT01

    3. NM_001411458.1 → NP_001398387.1  homeobox protein PKNOX1 isoform 1

      Status: VALIDATED

      Source sequence(s)
      AC166172, AC166575
      UniProtKB/Swiss-Prot
      O70477, Q7TT01

    4. NM_001411459.1 → NP_001398388.1  homeobox protein PKNOX1 isoform 1

      Status: VALIDATED

      Source sequence(s)
      AC166172, AC166575
      UniProtKB/Swiss-Prot
      O70477, Q7TT01

    5. NM_001411460.1 → NP_001398389.1  homeobox protein PKNOX1 isoform 2

      Status: VALIDATED

      Source sequence(s)
      AC166172, AC166575
      UniProtKB/TrEMBL
      A0A494B9U0
      Related
      ENSMUSP00000157748.2, ENSMUST00000237248.2

    6. NM_001411461.1 → NP_001398390.1  homeobox protein PKNOX1 isoform 2

      Status: VALIDATED

      Source sequence(s)
      AC166172, AC166575
      UniProtKB/TrEMBL
      A0A494B9U0

    7. NM_001411462.1 → NP_001398391.1  homeobox protein PKNOX1 isoform 2

      Status: VALIDATED

      Source sequence(s)
      AC166172, AC166575
      UniProtKB/TrEMBL
      A0A494B9U0

    8. NM_001411463.1 → NP_001398392.1  homeobox protein PKNOX1 isoform 2

      Status: VALIDATED

      Source sequence(s)
      AC166172, AC166575
      UniProtKB/TrEMBL
      A0A494B9U0

    9. NM_001411464.1 → NP_001398393.1  homeobox protein PKNOX1 isoform 3

      Status: VALIDATED

      Source sequence(s)
      AC166172, AC166575

    10. NM_001411465.1 → NP_001398394.1  homeobox protein PKNOX1 isoform 3

      Status: VALIDATED

      Source sequence(s)
      AC166172, AC166575

    11. NM_001411466.1 → NP_001398395.1  homeobox protein PKNOX1 isoform 4

      Status: VALIDATED

      Source sequence(s)
      AC166172, AC166575

    12. NM_001411467.1 → NP_001398396.1  homeobox protein PKNOX1 isoform 5

      Status: VALIDATED

      Source sequence(s)
      AC166172, AC166575

    13. NM_001411468.1 → NP_001398397.1  homeobox protein PKNOX1 isoform 6

      Status: VALIDATED

      Source sequence(s)
      AC166172, AC166575
      Related
      ENSMUSP00000157491.2, ENSMUST00000237038.2

    14. NM_001411469.1 → NP_001398398.1  homeobox protein PKNOX1 isoform 6

      Status: VALIDATED

      Source sequence(s)
      AC166172, AC166575

    15. NM_001411864.1 → NP_001398793.1  homeobox protein PKNOX1 isoform 1

      Status: VALIDATED

      Source sequence(s)
      AC166172, AC166575
      UniProtKB/Swiss-Prot
      O70477, Q7TT01
      Related
      ENSMUSP00000134852.2, ENSMUST00000175806.9

    16. NM_016670.4 → NP_057879.2  homeobox protein PKNOX1 isoform 1

      See identical proteins and their annotated locations for NP_057879.2

      Status: VALIDATED

      Description
      Transcript Variant: This variant (1) encodes the functional protein.
      Source sequence(s)
      AC166172, AC166575
      Consensus CDS
      CCDS28608.1
      UniProtKB/Swiss-Prot
      O70477, Q7TT01
      Related
      ENSMUSP00000094966.4, ENSMUST00000097352.11
      Conserved Domains (2) summary
      pfam05920
      Location:277 → 316
      Homeobox_KN; Homeobox KN domain
      pfam16493
      Location:80 → 165
      Meis_PKNOX_N; N-terminal of Homeobox Meis and PKNOX1

    RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCm39 C57BL/6J

    Genomic

    1. NC_000083.7 Reference GRCm39 C57BL/6J

      Range
      31783697..31826665
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. XM_036160407.1 → XP_036016300.1  homeobox protein PKNOX1 isoform X4

      Conserved Domains (2) summary
      pfam05920
      Location:274 → 313
      Homeobox_KN; Homeobox KN domain
      pfam16493
      Location:80 → 165
      Meis_PKNOX_N; N-terminal of Homeobox Meis and PKNOX1

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NR_027493.1: Suppressed sequence

      Description
      NR_027493.1: This RefSeq was removed because it is now thought that this transcript does encode a protein.
    Nucleotide Protein Strain
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O70477.3 GenPept UniProtKB/Swiss-Prot:O70477

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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