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ARHGAP25 Rho GTPase activating protein 25 [ Homo sapiens (human) ]

Gene ID: 9938, updated on 3-Apr-2024

Summary

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Official Symbol
ARHGAP25provided by HGNC
Official Full Name
Rho GTPase activating protein 25provided by HGNC
Primary source
HGNC:HGNC:28951
See related
Ensembl:ENSG00000163219 MIM:610587; AllianceGenome:HGNC:28951
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
KAIA0053; HEL-S-308
Summary
ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008]
Expression
Broad expression in lymph node (RPKM 20.1), spleen (RPKM 18.9) and 23 other tissues See more
Orthologs
mouse all
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Genomic context

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Location:
2p13.3
Exon count:
15
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 2 NC_000002.12 (68710544..68826833)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 2 NC_060926.1 (68722999..68839279)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (68961943..69053965)

Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene transmembrane and coiled-coil domain family 1 pseudogene Neighboring gene prokineticin receptor 1 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:68906264-68906888 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:68906889-68907513 Neighboring gene Sharpr-MPRA regulatory region 5025 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15958 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15959 Neighboring gene NANOG hESC enhancer GRCh37_chr2:68971645-68972146 Neighboring gene uncharacterized LOC105374790 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15960 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15961 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15962 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15963 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15964 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:69042669-69043169 Neighboring gene CRISPRi-validated cis-regulatory element chr2.2482 Neighboring gene long intergenic non-protein coding RNA 1890 Neighboring gene long intergenic non-protein coding RNA 1888 Neighboring gene ReSE screen-validated silencer GRCh37_chr2:69098345-69098505 Neighboring gene bone morphogenetic protein 10

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. A novel BRET-Based GAP assay reveals phosphorylation-dependent regulation of the RAC-specific GTPase activating protein ARHGAP25. Wisniewski É, et al. FASEB J, 2022 Nov. PMID 36190314
  2. ARHGAP25: A negative regulator of colorectal cancer (CRC) metastasis via the Wnt/β-catenin pathway. Tao L, et al. Eur J Pharmacol, 2019 Sep 5. PMID 31228451
  3. Relationship between the expression of ARHGAP25 and RhoA in non-small cell lung cancer and vasculogenic mimicry. Shi F, et al. BMC Pulm Med, 2022 Oct 7. PMID 36207695, Free PMC Article
  4. ARHGAP25 Inhibits Pancreatic Adenocarcinoma Growth by Suppressing Glycolysis via AKT/mTOR Pathway. Huang WK, et al. Int J Biol Sci, 2021. PMID 33994864, Free PMC Article
  5. Identification and characterization of ARHGAP24 and ARHGAP25 genes in silico. Katoh M, et al. Int J Mol Med, 2004 Aug. PMID 15254788

See all (17) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. ARHGAP25 suppresses the development of breast cancer by an ARHGAP25/Wnt/ASCL2 feedback loop.
    Title: ARHGAP25 suppresses the development of breast cancer by an ARHGAP25/Wnt/ASCL2 feedback loop.
  2. Relationship between the expression of ARHGAP25 and RhoA in non-small cell lung cancer and vasculogenic mimicry.
    Title: Relationship between the expression of ARHGAP25 and RhoA in non-small cell lung cancer and vasculogenic mimicry.
  3. A novel BRET-Based GAP assay reveals phosphorylation-dependent regulation of the RAC-specific GTPase activating protein ARHGAP25.
    Title: A novel BRET-Based GAP assay reveals phosphorylation-dependent regulation of the RAC-specific GTPase activating protein ARHGAP25.
  4. ARHGAP25 Inhibits Pancreatic Adenocarcinoma Growth by Suppressing Glycolysis via AKT/mTOR Pathway.
    Title: ARHGAP25 Inhibits Pancreatic Adenocarcinoma Growth by Suppressing Glycolysis via AKT/mTOR Pathway.
  5. ARHGAP25 negatively regulates the metastatic potential of CRC cells via the Wnt/beta-catenin pathway
    Title: ARHGAP25: A negative regulator of colorectal cancer (CRC) metastasis via the Wnt/β-catenin pathway.
  6. Observational study of gene-disease association. (HuGE Navigator)
    Title: Mutation of ARHGAP9 in patients with coronary spastic angina.
Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide association study on dimethylarginines reveals novel AGXT2 variants associated with heart rate variability but not with overall mortality.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables GTPase activator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in actin filament organization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in actin filament organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in activation of GTPase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in negative regulation of small GTPase mediated signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in negative regulation of small GTPase mediated signal transduction IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in phagocytosis, engulfment IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in phagocytosis, engulfment IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in signal transduction IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in phagocytic cup IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in phagocytic cup IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
rho GTPase-activating protein 25
Names
epididymis secretory protein Li 308
epididymis secretory sperm binding protein
rho-type GTPase-activating protein 25

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001007231.3 → NP_001007232.2  rho GTPase-activating protein 25 isoform a

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).
    Source sequence(s)
    AC097495, AK290396, BC032222, CD695161
    Consensus CDS
    CCDS33214.2
    UniProtKB/TrEMBL
    A0A2X0TVS2
    Related
    ENSP00000386911.3, ENST00000409202.8
    Conserved Domains (3) summary
    cd04390
    Location:156 → 354
    RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...
    cd13263
    Location:45 → 157
    PH_RhoGap25-like; Rho GTPase activating protein 25 and related proteins Pleckstrin homology (PH) domain
    pfam00169
    Location:47 → 149
    PH; PH domain

  2. NM_001166276.2 → NP_001159748.1  rho GTPase-activating protein 25 isoform c

    See identical proteins and their annotated locations for NP_001159748.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (c) is shorter and has a distinct N-terminus, compared to isoform a.
    Source sequence(s)
    BC032222
    Consensus CDS
    CCDS54364.1
    UniProtKB/TrEMBL
    A0A2X0TVS2
    Related
    ENSP00000386241.1, ENST00000409220.5
    Conserved Domains (3) summary
    cd04390
    Location:149 → 347
    RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...
    cd13263
    Location:38 → 150
    PH_RhoGap25-like; Rho GTPase activating protein 25 and related proteins Pleckstrin homology (PH) domain
    pfam00169
    Location:40 → 142
    PH; PH domain

  3. NM_001166277.2 → NP_001159749.1  rho GTPase-activating protein 25 isoform d

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) uses an alternate in-frame splice site and lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (d) that is shorter than isoform a.
    Source sequence(s)
    AC097495, AK297056, BC032222, CD695161
    Consensus CDS
    CCDS54363.1
    UniProtKB/TrEMBL
    A0A2X0TVS2
    Related
    ENSP00000420583.1, ENST00000467265.5
    Conserved Domains (3) summary
    cd04390
    Location:117 → 314
    RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...
    pfam00169
    Location:47 → 115
    PH; PH domain
    cl17171
    Location:45 → 120
    PH-like; Pleckstrin homology-like domain

  4. NM_001364819.1 → NP_001351748.1  rho GTPase-activating protein 25 isoform e

    Status: VALIDATED

    Source sequence(s)
    AC097495, AK290396, BC032222, CD695161, DA462915
    UniProtKB/Swiss-Prot
    A8K2Y1, B7Z498, E9PFQ7, G5E9G2, P42331, Q8IXQ2
    UniProtKB/TrEMBL
    A0A2X0TVS2, A0A384MTM1
    Conserved Domains (3) summary
    cd04390
    Location:155 → 353
    RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...
    cd13263
    Location:45 → 155
    PH_RhoGap25-like; Rho GTPase activating protein 25 and related proteins Pleckstrin homology (PH) domain
    cl25732
    Location:544 → 644
    SMC_N; RecF/RecN/SMC N terminal domain

  5. NM_001364820.1 → NP_001351749.1  rho GTPase-activating protein 25 isoform f

    Status: VALIDATED

    Source sequence(s)
    AC097495, AK297056, BC032222, BX340497, CD695161
    UniProtKB/TrEMBL
    A0A2X0TVS2
    Conserved Domains (3) summary
    cd04390
    Location:117 → 315
    RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...
    cl17171
    Location:45 → 122
    PH-like; Pleckstrin homology-like domain
    cl25732
    Location:506 → 606
    SMC_N; RecF/RecN/SMC N terminal domain

  6. NM_001364821.1 → NP_001351750.1  rho GTPase-activating protein 25 isoform g

    Status: VALIDATED

    Source sequence(s)
    AC097495, AK297056, BC032222, CR991724, DB243756
    UniProtKB/TrEMBL
    A0A2X0TVS2
    Conserved Domains (3) summary
    cd04390
    Location:130 → 328
    RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...
    cd13263
    Location:19 → 131
    PH_RhoGap25-like; Rho GTPase activating protein 25 and related proteins Pleckstrin homology (PH) domain
    cl25732
    Location:519 → 619
    SMC_N; RecF/RecN/SMC N terminal domain

  7. NM_014882.3 → NP_055697.1  rho GTPase-activating protein 25 isoform b

    See identical proteins and their annotated locations for NP_055697.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (b) is shorter and has a distinct N-terminus, compared to isoform a.
    Source sequence(s)
    BC032222, D29642
    Consensus CDS
    CCDS46312.1
    UniProtKB/TrEMBL
    A0A2X0TVS2, V9HWC8
    Related
    ENSP00000386863.3, ENST00000409030.7
    Conserved Domains (3) summary
    cd04390
    Location:148 → 346
    RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...
    cd13263
    Location:38 → 148
    PH_RhoGap25-like; Rho GTPase activating protein 25 and related proteins Pleckstrin homology (PH) domain
    pfam00169
    Location:40 → 142
    PH; PH domain

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000002.12 Reference GRCh38.p14 Primary Assembly

    Range
    68710544..68826833
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011533207.4 → XP_011531509.1  rho GTPase-activating protein 25 isoform X1

    See identical proteins and their annotated locations for XP_011531509.1

    UniProtKB/TrEMBL
    A0A2X0TVS2
    Conserved Domains (3) summary
    cd04390
    Location:130 → 328
    RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...
    cd13263
    Location:19 → 131
    PH_RhoGap25-like; Rho GTPase activating protein 25 and related proteins Pleckstrin homology (PH) domain
    cl25732
    Location:519 → 619
    SMC_N; RecF/RecN/SMC N terminal domain

  2. XM_011533209.3 → XP_011531511.1  rho GTPase-activating protein 25 isoform X2

    UniProtKB/TrEMBL
    H7C4P1
    Related
    ENSP00000417818.1, ENST00000497259.5
    Conserved Domains (1) summary
    cd04390
    Location:38 → 236
    RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...

  3. XM_017005426.2 → XP_016860915.1  rho GTPase-activating protein 25 isoform X3

    UniProtKB/TrEMBL
    C9JB56
    Conserved Domains (1) summary
    cl02570
    Location:1 → 80
    RhoGAP; RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when ...

  4. XM_011533210.4 → XP_011531512.1  rho GTPase-activating protein 25 isoform X3

    UniProtKB/TrEMBL
    C9JB56
    Related
    ENSP00000417467.1, ENST00000479844.1
    Conserved Domains (1) summary
    cl02570
    Location:1 → 80
    RhoGAP; RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when ...

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060926.1 Alternate T2T-CHM13v2.0

    Range
    68722999..68839279
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054344775.1 → XP_054200750.1  rho GTPase-activating protein 25 isoform X1

  2. XM_054344776.1 → XP_054200751.1  rho GTPase-activating protein 25 isoform X2

  3. XM_054344777.1 → XP_054200752.1  rho GTPase-activating protein 25 isoform X3

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P42331.2 GenPept UniProtKB/Swiss-Prot:P42331

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