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RNF7 ring finger protein 7 [ Homo sapiens (human) ]

Gene ID: 9616, updated on 5-Mar-2024

Summary

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Official Symbol
RNF7provided by HGNC
Official Full Name
ring finger protein 7provided by HGNC
Primary source
HGNC:HGNC:10070
See related
Ensembl:ENSG00000114125 MIM:603863; AllianceGenome:HGNC:10070
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
SAG; ROC2; rbx2; CKBBP1
Summary
The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Annotation information
Note: SAG (Gene ID: 6295) and RNF7 (Gene ID: 9616) share the SAG symbol/alias in common. SAG is a widely used alternative name for ring finger protein 7 (RNF7), which can be confused with the official symbol for SAG (S-antigen visual arrestin, GeneID 6295). [01 Jun 2018]
Expression
Ubiquitous expression in testis (RPKM 15.8), adrenal (RPKM 15.8) and 25 other tissues See more
Orthologs
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Genomic context

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See RNF7 in Genome Data Viewer
Location:
3q23
Exon count:
4
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 3 NC_000003.12 (141738298..141747560)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 3 NC_060927.1 (144485514..144494774)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 3 NC_000003.11 (141457140..141466402)

Chromosome 3 - NC_000003.12Genomic Context describing neighboring genes Neighboring gene RAS p21 protein activator 2 Neighboring gene YWHAQ pseudogene 6 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:141382574-141383094 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr3:141383095-141383614 Neighboring gene long intergenic non-protein coding RNA 2618 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20633 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20634 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20635 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:141456933-141457612 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr3:141457613-141458291 Neighboring gene TPT1 pseudogene 3 Neighboring gene ReSE screen-validated silencer GRCh37_chr3:141481495-141481669 Neighboring gene G protein-coupled receptor kinase 7 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20637 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 20638 Neighboring gene CRISPRi-validated cis-regulatory element chr3.4237 Neighboring gene CRISPRi-validated cis-regulatory element chr3.4238 Neighboring gene uncharacterized LOC105374134 Neighboring gene VISTA enhancer hs2041

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. RNF7 Facilitated the Tumorigenesis of Pancreatic Cancer by Activating PI3K/Akt Signaling Pathway. Hua H, et al. Oxid Med Cell Longev, 2023. PMID 36644576, Free PMC Article
  2. RNF7 inhibits apoptosis and sunitinib sensitivity and promotes glycolysis in renal cell carcinoma via the SOCS1/JAK/STAT3 feedback loop. Xiao C, et al. Cell Mol Biol Lett, 2022 May 13. PMID 35562668, Free PMC Article
  3. RNF7 knockdown inhibits prostate cancer tumorigenesis by inactivation of ERK1/2 pathway. Xiao Y, et al. Sci Rep, 2017 Mar 2. PMID 28252001, Free PMC Article
  4. Prognostic role of sensitive-to-apoptosis gene expression in rectal cancer. Ozden SA, et al. World J Gastroenterol, 2011 Nov 28. PMID 22171132, Free PMC Article
  5. Sensitive to apoptosis gene protein regulates ionizing radiation-induced apoptosis. Kim SY, et al. Biochimie, 2011 Feb. PMID 20933570

See all (107) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. RNF7 Facilitated the Tumorigenesis of Pancreatic Cancer by Activating PI3K/Akt Signaling Pathway.
    Title: RNF7 Facilitated the Tumorigenesis of Pancreatic Cancer by Activating PI3K/Akt Signaling Pathway.
  2. RNF7 inhibits apoptosis and sunitinib sensitivity and promotes glycolysis in renal cell carcinoma via the SOCS1/JAK/STAT3 feedback loop.
    Title: RNF7 inhibits apoptosis and sunitinib sensitivity and promotes glycolysis in renal cell carcinoma via the SOCS1/JAK/STAT3 feedback loop.
  3. We demonstrated that RNF7 knockdown induced growth suppression of prostate cancer cells and inactivated ERK1/2 pathway, which suggested RNF7 might be a potential novel therapeutic target for CRPC.
    Title: RNF7 knockdown inhibits prostate cancer tumorigenesis by inactivation of ERK1/2 pathway.
  4. Results identified RNF7 to interact with CARMA2 regulating its NF-kappaB-activating capacity. Mechanistically, RNF7 influences CARMA2 signaling by regulating the ubiquitination state of MALT1 and the NF-kappaB-regulatory molecule NEMO.
    Title: The E3 Ubiquitin Ligase RNF7 Negatively Regulates CARD14/CARMA2sh Signaling.
  5. This is the first report on a diagnostic test for simultaneous genotyping of IFNL3, ABCB11, and RNF7 in Chronic hepatitis C (CHC) patients. Reliable and inexpensive, the assay should provide useful information for the clinical management of CHC, like identification of patients at risk of rapid disease progression or with high chances of response to classic therapy.
    Title: Triplex High-Resolution Melting Assay for the Simultaneous Assessment of IFNL3 rs12979860, ABCB11 rs2287622, and RNF7 rs16851720 Genotypes in Chronic Hepatitis C Patients.
  6. Sag is a Kras-cooperating oncogene that promotes lung tumorigenesis
    Title: Inactivation of SAG/RBX2 E3 ubiquitin ligase suppresses KrasG12D-driven lung tumorigenesis.
  7. NEDD4-1 overexpression sensitizes cancer cells to etoposide-induced apoptosis by reducing SAG levels through targeted degradation. SAG is added to a growing list of NEDD4-1 substrates and mediates its biological function.
    Title: SAG/RBX2 is a novel substrate of NEDD4-1 E3 ubiquitin ligase and mediates NEDD4-1 induced chemosensitization.
  8. SAG/RBX2 E3 ligase complexes with UBCH10 and UBE2S ubiquitin-conjugating enzymes to ubiquitylate beta-TrCP1 via K11-linkage for degradation.
    Title: SAG/RBX2 E3 ligase complexes with UBCH10 and UBE2S E2s to ubiquitylate β-TrCP1 via K11-linkage for degradation.
  9. RNF7 gene variant is associated with the risk of developing liver fibrosis and cirrhosis in an Eastern European population.
    Title: PNPLA3 and RNF7 Gene Variants are Associated with the Risk of Developing Liver Fibrosis and Cirrhosis in an Eastern European Population.
  10. MAF1, RNF7 and SETD3 are identified as PCNA-associated proteins in human cells and given this interaction with PCNA, Maf1, RNF7, and SetD3 are potentially involved in DNA replication, DNA repair, or associated processes.
    Title: A fluorescent bimolecular complementation screen reveals MAF1, RNF7 and SETD3 as PCNA-associated proteins in human cells.
Submit: New GeneRIF Correction See all GeneRIFs (23)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide association study identifies variants associated with progression of liver fibrosis from HCV infection.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Protein interactions

Protein Gene Interaction Pubs
Vif vif Rbx1 I44A and Rbx2 I52A mutants disrupt the level of HIV-1 Vif-mediated APOBEC3G degradation in cells PubMed
vif CUL5/RBX2/ELOB/ELOC/Vif/CBF-beta complex catalyzes polyubiquitin chain formation on A3G in the presence of ubiquitin E2 UBE2R1 (CDC34) or UBCH5b (UBE2D2) PubMed
vif UBE2F and RBX2 are required for activation of the polyubiquitin synthesis activity of Vif/CBF-beta/CUL5, leading to HIV-1 Vif-mediated degradation of A3G in cells PubMed
vif HIV-1 Vif can assemble into the Cul5-containing E3 ligase, the CUL5-RBX2-CBF-beta-ELOB-ELOC complex, in the presence of CBF-beta PubMed
vif HIV-1 Vif is identified to have a physical interaction with ring finger protein 7 (RNF7; RBX2) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables NEDD8 ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables NEDD8 transferase activity TAS
Traceable Author Statement
more info
  
enables copper ion binding TAS
Traceable Author Statement
more info
 PubMed 
enables cullin family protein binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables cullin family protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ubiquitin protein ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in post-translational protein modification TAS
Traceable Author Statement
more info
  
involved_in protein neddylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein ubiquitination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein ubiquitination IEA
Inferred from Electronic Annotation
more info
  
involved_in response to redox state TAS
Traceable Author Statement
more info
 PubMed 
involved_in ubiquitin-dependent protein catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
part_of Cul5-RING ubiquitin ligase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of Cul5-RING ubiquitin ligase complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm NAS
Non-traceable Author Statement
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
  
located_in cytosol TAS
Traceable Author Statement
more info
  
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus NAS
Non-traceable Author Statement
more info
 PubMed 

General protein information

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Preferred Names
RING-box protein 2
Names
CKII beta-binding protein 1
regulator of cullins 2
sensitive to apoptosis gene protein
sensitive to apoptosis zinc RING finger protein SAG
sensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2
zinc RING finger protein SAG

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001201370.2 → NP_001188299.1  RING-box protein 2 isoform 4

    See identical proteins and their annotated locations for NP_001188299.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1.
    Source sequence(s)
    AC112771, AW150174, BG742338, DB272382
    Consensus CDS
    CCDS56283.1
    UniProtKB/TrEMBL
    B2R4X4
    Related
    ENSP00000419084.1, ENST00000480908.1
    Conserved Domains (1) summary
    cl17238
    Location:47 → 90
    RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain

  2. NM_014245.5 → NP_055060.1  RING-box protein 2 isoform 1

    See identical proteins and their annotated locations for NP_055060.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (1).
    Source sequence(s)
    AC112771, AW150174, BG742338
    Consensus CDS
    CCDS3118.1
    UniProtKB/Swiss-Prot
    A8K1H9, A8MTB5, C9JYL3, D3DNF7, D3DNF8, Q9BXN8, Q9UBF6, Q9Y5M7
    UniProtKB/TrEMBL
    B2R4X4
    Related
    ENSP00000273480.3, ENST00000273480.4
    Conserved Domains (1) summary
    cd16466
    Location:47 → 106
    RING-H2_RBX2; RING finger, H2 subclass, found in RING-box protein 2 (RBX2) and similar proteins

  3. NM_183237.3 → NP_899060.1  RING-box protein 2 isoform 3

    See identical proteins and their annotated locations for NP_899060.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) uses an alternate splice site in the coding region compared to variant 1, which causes a frameshift. The resulting shorter isoform (3) has a distinct C-terminus, as compared to isoform 1.
    Source sequence(s)
    AC112771, AW150174, BG716023, BG742338
    Consensus CDS
    CCDS43158.1
    UniProtKB/Swiss-Prot
    Q9UBF6
    Related
    ENSP00000376725.3, ENST00000393000.3
    Conserved Domains (1) summary
    cl17238
    Location:28 → 55
    RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain

RNA

  1. NR_037702.2 RNA Sequence

    Status: REVIEWED

    Description
    Transcript Variant: This variant (5) contains an alternate exon compared to variant 1, that causes a frameshift. The resulting transcript is a nonsense-mediated mRNA decay candidate and is not thought to be protein-coding.
    Source sequence(s)
    AC112771, AF312226, AW150174, BG742338

  2. NR_037703.2 RNA Sequence

    Status: REVIEWED

    Description
    Transcript Variant: This variant (6) uses an alternate splice junction and contains an alternate exon compared to variant 1, that causes a frameshift. The resulting transcript is a nonsense-mediated mRNA decay candidate and is not thought to be protein-coding.
    Source sequence(s)
    AC112771, AW150174, BG720797, BG742338

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000003.12 Reference GRCh38.p14 Primary Assembly

    Range
    141738298..141747560
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

RNA

  1. XR_007095770.1 RNA Sequence

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060927.1 Alternate T2T-CHM13v2.0

    Range
    144485514..144494774
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

RNA

  1. XR_008486859.1 RNA Sequence

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_183063.1: Suppressed sequence

    Description
    NM_183063.1: This RefSeq was permanently suppressed because it is a nonsense-mediated mRNA decay (NMD) candidate.
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9UBF6.1 GenPept UniProtKB/Swiss-Prot:Q9UBF6

Gene LinkOut

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