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lpoB outer membrane lipoprotein - activator of MrcB activity [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 948536, updated on 19-Sep-2024

Summary

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Official Symbol
lpoB
Official Full Name
outer membrane lipoprotein - activator of MrcB activity
Primary source
ECOCYC:G6565
Locus tag
b1105
See related
ASAP:ABE-0003733
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1091; ycfM
Summary
First 19 aa are a type II signal peptide. [More information is available at EcoGene: EG13431].
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Genomic context

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Sequence:
NC_000913.3 (1162638..1163279)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene purine nucleoside phosphoramidase Neighboring gene DUF1425 domain-containing protein YcfL Neighboring gene thiamine kinase Neighboring gene beta-N-acetylglucosaminidase Neighboring gene PF05728 family protein YcfP

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Site-Specific Immobilization of the Peptidoglycan Synthase PBP1B on a Surface Plasmon Resonance Chip Surface. Van't Veer IL, et al. Chembiochem, 2016 Dec 2. PMID 27709766, Free PMC Article
  2. Cofactor bypass variants reveal a conformational control mechanism governing cell wall polymerase activity. Markovski M, et al. Proc Natl Acad Sci U S A, 2016 Apr 26. PMID 27071112, Free PMC Article
  3. Outer-membrane lipoprotein LpoB spans the periplasm to stimulate the peptidoglycan synthase PBP1B. Egan AJ, et al. Proc Natl Acad Sci U S A, 2014 Jun 3. PMID 24821816, Free PMC Article
  4. Induced conformational changes activate the peptidoglycan synthase PBP1B. Egan AJF, et al. Mol Microbiol, 2018 Nov. PMID 30044025, Free PMC Article
  5. Upregulation of PBP1B and LpoB in cysB Mutants Confers Mecillinam (Amdinocillin) Resistance in Escherichia coli. Thulin E, et al. Antimicrob Agents Chemother, 2019 Oct. PMID 31332059, Free PMC Article

See all (14) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Upregulation of PBP1B and LpoB in cysB mutants confers mecillinam resistance in Escherichia coli.
    Title: Upregulation of PBP1B and LpoB in cysB Mutants Confers Mecillinam (Amdinocillin) Resistance in Escherichia coli.
  2. The lipoprotein LpoB is required to activate PBP1B, which is a major, bi-functional peptidoglycan synthase with glycan chain polymerising (glycosyltransferase) and peptide cross-linking (transpeptidase) activities. The authors show how the binding of LpoB to the regulatory UB2H domain of PBP1B activates both activities.
    Title: Induced conformational changes activate the peptidoglycan synthase PBP1B.
  3. Finally, phenotypic analysis of strains carrying a PBP1b* variant revealed that the PBP1b-LpoB complex is most likely not providing an important physical link between the inner and outer membranes at the division site, as has been previously proposed.
    Title: Cofactor bypass variants reveal a conformational control mechanism governing cell wall polymerase activity.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables enzyme activator activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables enzyme activator activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables enzyme binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables enzyme regulator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables enzyme regulator activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables enzyme regulator activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in peptidoglycan biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within peptidoglycan biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in peptidoglycan biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in peptidoglycan biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of cell shape IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cell outer membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cell outer membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in periplasmic side of cell outer membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in periplasmic side of cell outer membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in periplasmic side of cell outer membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
outer membrane lipoprotein - activator of MrcB activity

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1162638..1163279
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415623.1 outer membrane lipoprotein - activator of MrcB activity [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415623.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    Q1RD49
    Conserved Domains (1) summary
    COG3417
    Location:1 → 213
    LpoB; Outer membrane lipoprotein LpoB, binds and activates PBP1b [Cell wall/membrane/envelope biogenesis]
Nucleotide Protein
Heading Accession and Version

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