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rpsL 30S ribosomal subunit protein S12 [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947845, updated on 19-Sep-2024

Summary

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Official Symbol
rpsL
Official Full Name
30S ribosomal subunit protein S12
Primary source
ECOCYC:EG10911
Locus tag
b3342
See related
ASAP:ABE-0010919
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
asuB; ECK3329; strA
Summary
Mutations in regions 41-45 (except when lys42 is replaced with arg), 87-93 and 53 result in streptomycin resistance. [More information is available at EcoGene: EG10911]. The S12 protein is a component of the 30S subunit of the ribosome and plays a role in translational accuracy. [More information is available at EcoCyc: EG10911].
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Genomic context

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Sequence:
NC_000913.3 (3474178..3474552, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene elongation factor G Neighboring gene 30S ribosomal subunit protein S7 Neighboring gene sulfurtransferase complex subunit TusB Neighboring gene sulfurtransferase complex subunit TusC Neighboring gene sulfurtransferase complex subunit TusD

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A mutation in the ribosomal protein uS12 reveals novel functions of its universally conserved PNSA loop. Datta M, et al. Mol Microbiol, 2021 Jun. PMID 33368752
  2. Ribosomal mutations affecting the translation of genes that use non-optimal codons. Pelchovich G, et al. FEBS J, 2014 Aug. PMID 24966114
  3. The N-terminal extension of S12 influences small ribosomal subunit assembly in Escherichia coli. Calidas D, et al. RNA, 2014 Mar. PMID 24442609, Free PMC Article
  4. Structures of the bacterial ribosome in classical and hybrid states of tRNA binding. Dunkle JA, et al. Science, 2011 May 20. PMID 21596992, Free PMC Article
  5. Synthesis of beta-(S-methyl)thioaspartic acid and derivatives. Heredia-Moya J, et al. Bioorg Med Chem, 2008 Jun 1. PMID 18468905, Free PMC Article

See all (91) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A mutation in the ribosomal protein uS12 reveals novel functions of its universally conserved PNSA loop.
    Title: A mutation in the ribosomal protein uS12 reveals novel functions of its universally conserved PNSA loop.
  2. The semi error-restrictive mutation (K87R), within the rpsL gene, causes an increase in the abundance and activity of proteins encoded by exogenous genes containing non-optimal AT-rich codons in E. coli.
    Title: Ribosomal mutations affecting the translation of genes that use non-optimal codons.
  3. The authors provide evidence that mutant IF2 specifically interacts with rpsL31 in cold, leading to a bacteriostatic effect on host cells.
    Title: A novel mutation spatially remote from the G-domain in IF2 affects the cold stress adaptation of Escherichia coli.
  4. K42A and R53A variants of RpsL, were substantially resistant to the miscoding effects of paromomycin.
    Title: Mutational analysis of S12 protein and implications for the accuracy of decoding by the ribosome.
  5. N-terminus verified by Edman degradation on mature peptide
    Title: Primary stucture of protein S12 from the small Escherichia coli ribosomal subunit.
  6. combination of S12 & otherwise benign mutations at positions C1409-G1491 in 16S rRNA severely compromises cell growth while level & range of aminoglycoside resistances conferred by the G1491U/C substitutions is markedly increased by a mutant S12 protein.
    Title: Modulation of 16S rRNA function by ribosomal protein S12.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables misfolded RNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables rRNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables rRNA binding IEA
Inferred from Electronic Annotation
more info
  
enables structural constituent of ribosome IBA
Inferred from Biological aspect of Ancestor
more info
  
enables structural constituent of ribosome IDA
Inferred from Direct Assay
more info
 PubMed 
enables structural constituent of ribosome IEA
Inferred from Electronic Annotation
more info
  
enables tRNA binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within Group I intron splicing IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within RNA folding IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cytoplasmic translation NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in maintenance of translational fidelity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within positive regulation of RNA splicing IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in response to antibiotic IEA
Inferred from Electronic Annotation
more info
  
involved_in translation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in translation IEA
Inferred from Electronic Annotation
more info
  
involved_in translation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
part_of cytosolic small ribosomal subunit IDA
Inferred from Direct Assay
more info
 PubMed 
part_of cytosolic small ribosomal subunit IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of ribonucleoprotein complex IEA
Inferred from Electronic Annotation
more info
  
is_active_in ribosome IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in ribosome IEA
Inferred from Electronic Annotation
more info
  
part_of small ribosomal subunit IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
30S ribosomal subunit protein S12

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3474178..3474552 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417801.1 30S ribosomal subunit protein S12 [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417801.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6C9QFZ1
    Conserved Domains (1) summary
    PRK05163
    Location:1 → 124
    rpsL; 30S ribosomal protein S12; Validated
Nucleotide Protein
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Other Literature Sources
Molecular Biology Databases
Research Materials