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ygjK glycoside hydrolase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 947596, updated on 19-Sep-2024

Summary

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Official Symbol
ygjK
Official Full Name
glycoside hydrolase
Primary source
ECOCYC:G7599
Locus tag
b3080
See related
ASAP:ABE-0010118
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK3070
Summary
The first 22 aa are predicted to be a type I signal peptide. [More information is available at EcoGene: EG12722]. YgjK belongs to the glycoside hydrolase family 63 and was shown to possess -1,3-glucosyl hydrolase activity. [More information is available at EcoCyc: G7599].
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Genomic context

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Sequence:
NC_000913.3 (3228888..3231239)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene putative transporter YgjI Neighboring gene protein YgjJ Neighboring gene 2,4-dienoyl-CoA reductase Neighboring gene antitoxin/DNA-binding transcriptional repressor HigA Neighboring gene ribosome-dependent mRNA interferase toxin HigB

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Crystal structure of the enzyme-product complex reveals sugar ring distortion during catalysis by family 63 inverting α-glycosidase. Miyazaki T, et al. J Struct Biol, 2016 Dec. PMID 27688023
  2. Structural insights into the substrate specificity and function of Escherichia coli K12 YgjK, a glucosidase belonging to the glycoside hydrolase family 63. Kurakata Y, et al. J Mol Biol, 2008 Aug 1. PMID 18586271
  3. Crystallization and preliminary X-ray analysis of Escherichia coli K12 YgjK protein, a member of glycosyl hydrolase family 63. Tonozuka T, et al. Acta Crystallogr D Biol Crystallogr, 2004 Jul. PMID 15213393
  4. Glycosyltransferases, glycoside hydrolases: surprise, surprise! Henrissat B, et al. Curr Opin Struct Biol, 2008 Oct. PMID 18822375
  5. Over 1000 genes are involved in the DNA damage response of Escherichia coli. Khil PP, et al. Mol Microbiol, 2002 Apr. PMID 11967071

See all (9) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Comparison between these structures and the previously determined enzyme-substrate complex structure reveals that the glucose pyranose ring might be distorted immediately after nucleophilic attack by a water molecule. These structures represent the first enzyme-product complex for the GH63 family, as well as the structurally-related glycosidases, and it may provide insight into the catalytic mechanism of these enzymes
    Title: Crystal structure of the enzyme-product complex reveals sugar ring distortion during catalysis by family 63 inverting α-glycosidase.
  2. Escherichia coli K12 YgjK protein, a member of GH family 63, was overexpressed, purified and crystallized using the vapour-diffusion method and diffraction data were collected to 1.8 A resolution
    Title: Crystallization and preliminary X-ray analysis of Escherichia coli K12 YgjK protein, a member of glycosyl hydrolase family 63.
Submit: New GeneRIF Correction

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables alpha,alpha-trehalase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables alpha,alpha-trehalase activity IEA
Inferred from Electronic Annotation
more info
  
enables glucosidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables hydrolase activity, acting on glycosyl bonds IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within DNA damage response IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in oligosaccharide catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in trehalose catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in trehalose metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of glucosidase complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
glycoside hydrolase

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    3228888..3231239
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_417551.1 glycoside hydrolase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_417551.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6N8NBF4
    Conserved Domains (1) summary
    PRK10137
    Location:1 → 782
    PRK10137; alpha-glucosidase; Provisional
Nucleotide Protein
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