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purF amidophosphoribosyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 946794, updated on 10-Oct-2024

Summary

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Official Symbol
purF
Official Full Name
amidophosphoribosyltransferase
Primary source
ECOCYC:EG10794
Locus tag
b2312
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ade; ECK2306; purC
Summary
The glutamine amide transfer domain has been identified near the N-terminal end . purF is a component of a polycistronic mRNA . [More information is available at EcoCyc: EG10794].
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Genomic context

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Sequence:
NC_000913.3 (2428721..2430238, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene lysine/arginine/ornithine ABC transporter periplasmic binding protein Neighboring gene flavin prenyltransferase Neighboring gene colicin V production protein Neighboring gene cell division protein DedD Neighboring gene bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Coupled formation of an amidotransferase interdomain ammonia channel and a phosphoribosyltransferase active site. Krahn JM, et al. Biochemistry, 1997 Sep 16. PMID 9333323
  2. Glutamine phosphoribosylpyrophosphate amidotransferase from Escherichia coli. Purification and properties. Messenger LJ, et al. J Biol Chem, 1979 May 10. PMID 372191
  3. Amino-terminal deletions define a glutamine amide transfer domain in glutamine phosphoribosylpyrophosphate amidotransferase and other PurF-type amidotransferases. Mei BG, et al. J Bacteriol, 1990 Jun. PMID 2188964, Free PMC Article
  4. Nucleotide sequence of the Escherichia coli purF gene encoding amidophosphoribosyltransferase for de novo purine nucleotide synthesis. Sampei G, et al. Nucleic Acids Res, 1988 Sep 12. PMID 3047685, Free PMC Article
  5. Nucleotide sequence of Escherichia coli purF and deduced amino acid sequence of glutamine phosphoribosylpyrophosphate amidotransferase. Tso JY, et al. J Biol Chem, 1982 Apr 10. PMID 6277938

See all (28) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The ammonia channel in 5'-phosphoribosylpyrophosphate amidotransferase is regarded as a "pipe" through which ammonia travels in the absence of an external "driving" potential.
    Title: Computational studies of ammonia channel function in glutamine 5'-phosphoribosylpyrophosphate amidotransferase.
  2. phosphoribosylanthranilate isomerase
    Title: A study in molecular contingency: glutamine phosphoribosylpyrophosphate amidotransferase is a promiscuous and evolvable phosphoribosylanthranilate isomerase.
  3. N-terminus verified by Edman degradation on mature peptide
    Title: Glutamine phosphoribosylpyrophosphate amidotransferase from cloned Escherichia coli purF. NH2-terminal amino acid sequence, identification of the glutamine site, and trace metal analysis.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables amidophosphoribosyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables amidophosphoribosyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables amidophosphoribosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables glycosyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glycosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables guanosine tetraphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables magnesium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in 'de novo' IMP biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within glutamine metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in glutamine metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within glutamine metabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in purine nucleobase biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within purine nucleotide biosynthetic process EXP
Inferred from Experiment
more info
 PubMed 
involved_in purine nucleotide biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within purine nucleotide biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in purine nucleotide biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within purine nucleotide biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol HDA PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
amidophosphoribosyltransferase
NP_416815.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    2428721..2430238 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_416815.1 amidophosphoribosyltransferase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_416815.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AH83, A0AAN3GEX0
    Conserved Domains (1) summary
    PRK09246
    Location:1 → 505
    PRK09246; amidophosphoribosyltransferase; Provisional
Nucleotide Protein
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