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rnb RNase II [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945864, updated on 19-Sep-2024

Summary

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Official Symbol
rnb
Official Full Name
RNase II
Primary source
ECOCYC:EG11620
Locus tag
b1286
See related
ASAP:ABE-0004320
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK1281
Summary
Ribonuclease II (RNase II) is an exonuclease that cleaves RNA from the 3' end to produce ribonucleoside 5'-monophosphates. [More information is available at EcoCyc: EG11620].
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Genomic context

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Sequence:
NC_000913.3 (1346978..1348912, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein YmiD Neighboring gene cyclic di-GMP phosphodiesterase PdeR Neighboring gene putative protein YmiB Neighboring gene putative peroxidase YciW Neighboring gene enoyl-[acyl-carrier-protein] reductase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A single mutation in Escherichia coli ribonuclease II inactivates the enzyme without affecting RNA binding. Amblar M, et al. FEBS J, 2005 Jan. PMID 15654875
  2. Escherichia coli ribonuclease II. Cannistraro VJ, et al. Methods Enzymol, 2001. PMID 11586905
  3. Expression, purification, crystallization and preliminary diffraction data characterization of Escherichia coli ribonuclease II (RNase II). McVey CE, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2006 Jul 1. PMID 16820694, Free PMC Article
  4. Precise physical mapping of the Escherichia coli rnb gene, encoding ribonuclease II. Zilhão R, et al. Mol Gen Genet, 1995 Jul 28. PMID 7651348
  5. Construction and characterization of an absolute deletion mutant of Escherichia coli ribonuclease II. Piedade J, et al. FEMS Microbiol Lett, 1995 Apr 1. PMID 7758932

See all (84) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Reversible acetylation on Lys501 regulates the activity of RNase II.
    Title: Reversible acetylation on Lys501 regulates the activity of RNase II.
  2. We determined that the deletion of RNase II significantly affected 187 transcripts, while deletion of RNase R affects 202 transcripts and deletion of PNPase affected 226 transcripts
    Title: Next generation sequencing analysis reveals that the ribonucleases RNase II, RNase R and PNPase affect bacterial motility and biofilm formation in E. coli.
  3. Data show that RNase II is associated with all of the known degradosome proteins.
    Title: The Escherichia coli major exoribonuclease RNase II is a component of the RNA degradosome.
  4. Data indicate that membrane association and self-interactions are both required for the RNase II cellular organization.
    Title: Membrane association via an amino-terminal amphipathic helix is required for the cellular organization and function of RNase II.
  5. The authors propose that RNase II degrades mRNA to the downstream border of paused ribosomes, facilitating cleavage of the A-site codon by an unknown RNase.
    Title: RNase II is important for A-site mRNA cleavage during ribosome pausing.
  6. The crystal structure of E. coli RNase II reveals an architecture reminiscent of the RNA exosome.
    Title: Structural basis for processivity and single-strand specificity of RNase II.
  7. A functionl characterization of three different domains assigned to the Escherichia coli RNase II - two RNA-binding domains at each end of the protein (CSD and S1), and a central RNB catalytic domain - was performed
    Title: Characterization of the functional domains of Escherichia coli RNase II.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 3'-5' exonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables exonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables exoribonuclease II activity IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in RNA catabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in mRNA catabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within tRNA decay EXP
Inferred from Experiment
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
RNase II
NP_415802.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    1346978..1348912 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415802.1 RNase II [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415802.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5D8MRN2
    Conserved Domains (1) summary
    PRK05054
    Location:1 → 643
    PRK05054; exoribonuclease II; Provisional
Nucleotide Protein
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