nfsA [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: nfsA
Primary source: ECOCYC:EG11261
Locus tag: b0851
See related: ASAP:ABE-0002900
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK0842; mda18; mdaA; ybjB
Summary: NfsA overexpression results in resistance to cytotoxic drugs includingAdriamycin and the tumoricidal agent DMP 840. nfsA is co-transcribed with ybjC. nfsA, nfsB, rutE, and ydjA are paralogs. [More information is available at EcoGene: EG11261]. The nfsA gene encodes the major oxygen-insensitive nitroreductase present in Escherichia coli. [More information is available at EcoCyc: EG11261].
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Genomic context

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Sequence:: NC_000913.3 (891184..891906)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Alterations in chromosomal genes nfsA, nfsB, and ribE are associated with nitrofurantoin resistance in Escherichia coli from the United Kingdom.
Title: Alterations in chromosomal genes nfsA, nfsB, and ribE are associated with nitrofurantoin resistance in Escherichia coli from the United Kingdom.
The structures of E. coli NfsA bound to the antibiotic nitrofurantoin; to 1,4-benzoquinone and to FMN.
Title: The structures of E. coli NfsA bound to the antibiotic nitrofurantoin; to 1,4-benzoquinone and to FMN.
Mutations in nfsA and nfsB were the main mechanisms leading to nitrofurantoin resistance, and overexpression of the oqxAB gene might help to further increase the minimum inhibitory concentrations of nitrofurantoin.
Title: Unravelling mechanisms of nitrofurantoin resistance and epidemiological characteristics among Escherichia coli clinical isolates.
reduction of quinones by NfsA is most consistent with a single-step (H(-)) hydride transfer mechanism
Title: Reduction of quinones and nitroaromatic compounds by Escherichia coli nitroreductase A (NfsA): Characterization of kinetics and substrate specificity.
Findings revealed that Phe42 is important for maintaining NfsA activity and structure.
Title: Residue Phe42 is critical for the catalytic activity of Escherichia coli major nitroreductase NfsA.
NfsA and NfsB, but not NemA, are responsible for the conversion of ethyl 2-(2'-nitrophenoxy)acetate.
Title: Study of the role played by NfsA, NfsB nitroreductase and NemA flavin reductase from Escherichia coli in the conversion of ethyl 2-(2'-nitrophenoxy)acetate to 4-hydroxy-(2H)-1,4-benzoxazin-3(4H)-one (D-DIBOA), a benzohydroxamic acid with interesting biological properties.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables FMN binding	IDA Inferred from Direct Assay more info	PubMed
enables NAD(P)H dehydrogenase (quinone) activity	IDA Inferred from Direct Assay more info	PubMed
enables chromate reductase activity	IDA Inferred from Direct Assay more info	PubMed
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: NADPH-dependent nitro/quinone reductase NfsA

NP_415372.1

EC 1.6.5.10

NCBI Reference Sequences (RefSeq)

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Genome Annotation

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Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

891184..891906

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_415372.1 NADPH-dependent nitro/quinone reductase NfsA [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_415372.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A7A7AXF1

Conserved Domains (1) summary

PRK10765
Location:1 → 240

PRK10765; oxygen-insensitive NADPH nitroreductase