U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

ybfF esterase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945288, updated on 19-Sep-2024

Summary

Go to the top of the page Help
Official Symbol
ybfF
Official Full Name
esterase
Primary source
ECOCYC:EG11776
Locus tag
b0686
See related
ASAP:ABE-0002337
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0674
Summary
Thioesterase activity of YbfF with large substrates such as palmitoyl-CoA and malonyl-CoA was discovered in a high-throughput screen of purified proteins . [More information is available at EcoCyc: EG11776].
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Sequence:
NC_000913.3 (712038..712802, complement)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene flavodoxin 1 Neighboring gene ribbon-helix-helix domain-containing protein YbfE Neighboring gene negative modulator of initiation of replication Neighboring gene phosphoglucomutase

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Discovery of an Escherichia coli esterase with high activity and enantioselectivity toward 1,2-O-isopropylideneglycerol esters. Godinho LF, et al. Appl Environ Microbiol, 2011 Sep. PMID 21764964, Free PMC Article
  2. Rapid functional identification of putative genes based on the combined in vitro protein synthesis with mass spectrometry: a tool for functional genomics. Kim JH, et al. Anal Biochem, 2008 Apr 1. PMID 18258174
  3. High-resolution structure of ybfF from Escherichia coli K12: a unique substrate-binding crevice generated by domain arrangement. Park SY, et al. J Mol Biol, 2008 Mar 7. PMID 18215690
  4. Crystallization and preliminary X-ray diffraction analysis of ybfF, a new esterase from Escherichia coli K12. Park SY, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2007 Dec 1. PMID 18084091, Free PMC Article
  5. Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography. Fountoulakis M, et al. Electrophoresis, 1999 Aug. PMID 10493123

See all (14) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. This approach led to the identification of esterase YbfF as the major Escherichia coli enzyme responsible for the hydrolytic activity toward 1,2-O-isopropylideneglycerol esters.
    Title: Discovery of an Escherichia coli esterase with high activity and enantioselectivity toward 1,2-O-isopropylideneglycerol esters.
  2. The high-resolution structure of ybfF shows that the inserted helical bundle structure is critical for creating a large inner space, thereby providing the broad substrate spectrum toward large biomolecules.
    Title: High-resolution structure of ybfF from Escherichia coli K12: a unique substrate-binding crevice generated by domain arrangement.
  3. With two Ec_ybfF molecules in the asymmetric unit, the crystal volume per unit protein weight is 2.17 A(3) Da(-1), corresponding to a solvent content of 42%
    Title: Crystallization and preliminary X-ray diffraction analysis of ybfF, a new esterase from Escherichia coli K12.
Submit: New GeneRIF Correction

General gene information

Go to the top of the page Help

Homology

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables carboxylesterase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables carboxylic ester hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables thiolester hydrolase activity IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
esterase

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    712038..712802 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415212.1 esterase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415212.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A8S7RTH0
    Conserved Domains (1) summary
    PRK10673
    Location:1 → 254
    PRK10673; esterase
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.