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entH proofreading thioesterase in enterobactin biosynthesis [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 945215, updated on 25-Jan-2024

Summary

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Gene symbol
entH
Gene description
proofreading thioesterase in enterobactin biosynthesis
Primary source
ASAP:ABE-0002057
Locus tag
b0597
See related
ECOCYC:EG11105
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0590; ybdB
Summary
EntH is a member of the hotdog superfamily. [More information is available at EcoGene: EG11105]. EntH is a thioesterase that is involved in the biosynthesis of enterobactin . [More information is available at EcoCyc: EG11105].
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Genomic context

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Sequence:
NC_000913.3 (629300..629713)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 2,3-dihydroxybenzoate-AMP ligase Neighboring gene enterobactin synthase component B Neighboring gene 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase Neighboring gene pyruvate transporter CstA Neighboring gene PF04328 family protein YbdD

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Preferential hydrolysis of aberrant intermediates by the type II thioesterase in Escherichia coli nonribosomal enterobactin synthesis: substrate specificities and mutagenic studies on the active-site residues. Guo ZF, et al. Biochemistry, 2009 Mar 3. PMID 19193103
  2. In vitro kinetic analysis of substrate specificity in enterobactin biosynthetic lower pathway enzymes provides insight into the biochemical function of the hot dog-fold thioesterase EntH. Chen D, et al. Biochemistry, 2009 Jan 27. PMID 19119850, Free PMC Article
  3. Nucleotide sequence and transcriptional organization of the Escherichia coli enterobactin biosynthesis cistrons entB and entA. Nahlik MS, et al. J Bacteriol, 1989 Feb. PMID 2521621, Free PMC Article
  4. Reconstitution and characterization of the Escherichia coli enterobactin synthetase from EntB, EntE, and EntF. Gehring AM, et al. Biochemistry, 1998 Feb 24. PMID 9485415
  5. Cluster of genes controlling synthesis and activation of 2,3-dihydroxybenzoic acid in production of enterobactin in Escherichia coli. Nahlik MS, et al. J Bacteriol, 1987 Sep. PMID 3040680, Free PMC Article

See all (20) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Results show YbdB thioesterase activity could be required for cleaving wrongly charged molecules on the carrier protein EntB.
    Title: The hotdog thioesterase EntH (YbdB) plays a role in vivo in optimal enterobactin biosynthesis by interacting with the ArCP domain of EntB.
Submit: New GeneRIF Correction

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 1,4-dihydroxy-2-naphthoyl-CoA thioesterase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables acyl-CoA hydrolase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables acyl-CoA hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, acting on ester bonds IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables thiolester hydrolase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables thiolester hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in enterobactin biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within enterobactin biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
proofreading thioesterase in enterobactin biosynthesis

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    629300..629713
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_415129.1 proofreading thioesterase in enterobactin biosynthesis [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_415129.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A3L9GKK5
    Conserved Domains (1) summary
    PRK10254
    Location:1 → 137
    PRK10254; proofreading thioesterase EntH
Nucleotide Protein
Heading Accession and Version

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