F fusion protein [Henipavirus nipahense] - Gene

Summary

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Gene symbol: F
Gene description: fusion protein
Locus tag: Nvgp4
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Henipavirus nipahense
Lineage: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Orthoparamyxovirinae; Henipavirus
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Genomic context

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Sequence:: NC_002728.1 (6370..8706)

NC_002728.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_002728.1

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Using both coimmunoprecipitation and flow-cytometric strategies, we found a bidentate interaction between Nipah virus (NiV) G and F, where both the stalk and head regions of NiV G interact with F. This is a new structural-biological finding for the paramyxoviruses. Additionally, our studies disclosed regions of the NiV G and F glycoproteins dispensable for the G and F interactions.
Title: Multiple Strategies Reveal a Bidentate Interaction between the Nipah Virus Attachment and Fusion Glycoproteins.
Deletions of more than four residues in F protein did not affect F uptake or endosomal cleavage but downregulated the surface expression, likely by delaying the intracellular trafficking through endosomal-recycling compartments.
Title: Nipah virus fusion protein: Importance of the cytoplasmic tail for endosomal trafficking and bioactivity.
Data suggest that fusion of Nipah viruses with host cells is facilitated by two of viral membrane proteins, G and F proteins; G protein binds to human ephrins B2 and B3; this interaction triggers F protein to mediate virus-host fusion.
Title: Discerning intersecting fusion-activation pathways in the Nipah virus using machine learning.
These findings are consistent with a general mechanism of paramyxovirus fusion activation in which the stalk domain of the receptor binding protein is responsible for F activation.
Title: Identification of a region in the stalk domain of the nipah virus receptor binding protein that is critical for fusion activation.
These findings suggest that the G-F interaction is not mediated entirely by the stalk domain of G.
Title: Individual N-glycans added at intervals along the stalk of the Nipah virus G protein prevent fusion but do not block the interaction with the homologous F protein.
Activation of the Nipah virus fusion protein is mediated by cathepsin B within the endosome.
Title: Activation of the Nipah virus fusion protein in MDCK cells is mediated by cathepsin B within the endosome-recycling compartment.
The authors suggest that the continued engagement of the receptor binding protein is essential to F protein function until the onset of membrane merger.
Title: Spring-loaded model revisited: paramyxovirus fusion requires engagement of a receptor binding protein beyond initial triggering of the fusion protein.
The authors conclude that distribution of Nipah virus F and G glycoproteins is responsible for lateral virus spread in both, epithelial and endothelial cell monolayers.
Title: Nipah virus infection and glycoprotein targeting in endothelial cells.
galectin-1 reduces NiV-F mediated fusion of endothelial cells, and endogenous galectin-1 in endothelial cells is sufficient to inhibit syncytia formation
Title: Endothelial galectin-1 binds to specific glycans on nipah virus fusion protein and inhibits maturation, mobility, and function to block syncytia formation.
The results suggest that basolateral or bipolar F and G expression facilitates the spread of Nipah virus within epithelial cell monolayers, thereby contributing to efficient virus spread in mucosal surfaces in early and late phases of infection.
Title: Tyrosine residues in the cytoplasmic domains affect sorting and fusion activity of the Nipah virus glycoproteins in polarized epithelial cells.

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General gene information

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Homology

Orthologs from OrthoDB

General protein information

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Preferred Names: fusion protein

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_002728.1 Reference assembly

Range

6370..8706

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_112026.1 fusion protein [Henipavirus nipahense]

See identical proteins and their annotated locations for NP_112026.1

Status: PROVISIONAL

UniProtKB/Swiss-Prot

Q5K4D9, Q8QU00, Q9IH63

UniProtKB/TrEMBL

A0A1L7B8D7, E4Z9G2

Conserved Domains (1) summary

pfam00523
Location:29 → 495

Fusion_gly; Fusion glycoprotein F0