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RNF185 ring finger protein 185 [ Homo sapiens (human) ]

Gene ID: 91445, updated on 5-Mar-2024

Summary

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Official Symbol
RNF185provided by HGNC
Official Full Name
ring finger protein 185provided by HGNC
Primary source
HGNC:HGNC:26783
See related
Ensembl:ENSG00000138942 MIM:620096; AllianceGenome:HGNC:26783
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Summary
Enables ubiquitin binding activity; ubiquitin protein ligase activity; and ubiquitin-like protein conjugating enzyme binding activity. Involved in positive regulation of ERAD pathway; protein autoubiquitination; and ubiquitin-dependent ERAD pathway. Located in endoplasmic reticulum. [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in thyroid (RPKM 37.5), prostate (RPKM 19.7) and 25 other tissues See more
Orthologs
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Genomic context

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See RNF185 in Genome Data Viewer
Location:
22q12.2
Exon count:
8
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 22 NC_000022.11 (31160182..31207019)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 22 NC_060946.1 (31623670..31670571)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 22 NC_000022.10 (31556168..31603005)

Chromosome 22 - NC_000022.11Genomic Context describing neighboring genes Neighboring gene Sharpr-MPRA regulatory region 9733 Neighboring gene SNRPN pseudogene 2 Neighboring gene microRNA 3928 Neighboring gene NANOG-H3K27ac hESC enhancer GRCh37_chr22:31579617-31580130 Neighboring gene H3K27ac hESC enhancer GRCh37_chr22:31580131-31580643 Neighboring gene origin of replication in RNF185 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18849 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 13624 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:31608967-31609892 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18850 Neighboring gene LIM domain kinase 2 Neighboring gene RNA, U6 small nuclear 1128, pseudogene Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18851 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:31643479-31644369 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:31644370-31645259 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:31672194-31672774 Neighboring gene protein phosphatase 1 regulatory inhibitor subunit 14B pseudogene 1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. CRISPR screening of E3 ubiquitin ligases reveals Ring Finger Protein 185 as a novel tumor suppressor in glioblastoma repressed by promoter hypermethylation and miR-587. Lin K, et al. J Transl Med, 2022 Feb 19. PMID 35183197, Free PMC Article
  2. RNF185 modulates JWA ubiquitination and promotes gastric cancer metastasis. Qiu D, et al. Biochim Biophys Acta Mol Basis Dis, 2018 May. PMID 29481911
  3. The E3 ligase RNF185 negatively regulates osteogenic differentiation by targeting Dvl2 for degradation. Zhou Y, et al. Biochem Biophys Res Commun, 2014 May 9. PMID 24727453
  4. The E3 ubiquitin ligase RNF185 facilitates the cGAS-mediated innate immune response. Wang Q, et al. PLoS Pathog, 2017 Mar. PMID 28273161, Free PMC Article
  5. RNF185, a novel mitochondrial ubiquitin E3 ligase, regulates autophagy through interaction with BNIP1. Tang F, et al. PLoS One, 2011. PMID 21931693, Free PMC Article

See all (38) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. CRISPR screening of E3 ubiquitin ligases reveals Ring Finger Protein 185 as a novel tumor suppressor in glioblastoma repressed by promoter hypermethylation and miR-587.
    Title: CRISPR screening of E3 ubiquitin ligases reveals Ring Finger Protein 185 as a novel tumor suppressor in glioblastoma repressed by promoter hypermethylation and miR-587.
  2. increased RNF185 expression facilitated GC cell migration in vitro and promoted GC metastasis in vivo by downregulating JWA expression.
    Title: RNF185 modulates JWA ubiquitination and promotes gastric cancer metastasis.
  3. this study uncovers RNF185 as the first E3 ubiquitin ligase of cyclic GMP-AMP synthase (cGAS), shedding light on the regulation of cGAS activity in innate immune responses
    Title: The E3 ubiquitin ligase RNF185 facilitates the cGAS-mediated innate immune response.
  4. these results indicate that RNF185 negatively regulates osteogenesis through the degradation of Dvl2 and down-regulation of canonical Wnt signaling pathway and suggest a possible therapeutic target in osteoporosis.
    Title: The E3 ligase RNF185 negatively regulates osteogenic differentiation by targeting Dvl2 for degradation.
  5. data thus identify RNF185 and RNF5 as a novel E3 ligase module that is central to the control of CFTR degradation.
    Title: RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR).
  6. Human BNIP1 colocalizes with RNF185 at mitochondria and is polyubiquitinated by RNF185 through K63-based ubiquitin linkage in vivo.
    Title: RNF185, a novel mitochondrial ubiquitin E3 ligase, regulates autophagy through interaction with BNIP1.
Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Protein interactions

Protein Gene Interaction Pubs
Tat tat HIV-1 Tat downregulates the expression of ring finger protein 185 (RNF185) in human primary T cells PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • FLJ38628

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein-containing complex binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ubiquitin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ubiquitin protein ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ubiquitin protein ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ubiquitin protein ligase activity TAS
Traceable Author Statement
more info
  
enables ubiquitin-like protein conjugating enzyme binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ubiquitin-like protein conjugating enzyme binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in ERAD pathway IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in ERAD pathway IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in autophagy IEA
Inferred from Electronic Annotation
more info
  
involved_in defense response to virus IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in innate immune response IEA
Inferred from Electronic Annotation
more info
  
involved_in positive regulation of ERAD pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of innate immune response IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of type I interferon-mediated signaling pathway IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein K27-linked ubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein autoubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in transmembrane transport TAS
Traceable Author Statement
more info
  
involved_in ubiquitin-dependent protein catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in ubiquitin-dependent protein catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in endoplasmic reticulum IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endoplasmic reticulum membrane NAS
Non-traceable Author Statement
more info
 PubMed 
located_in endoplasmic reticulum membrane TAS
Traceable Author Statement
more info
  
located_in mitochondrial outer membrane IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
E3 ubiquitin-protein ligase RNF185
Names
BSK65-MONO1
BSK65-MONO2
BSK65-PANC1
BSK65-PANC2
BSK65-TEST1
BSK65-TEST2
BSK65-TEST3
RING-type E3 ubiquitin transferase RNF185
NP_001129297.1
NP_689480.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001135825.2 → NP_001129297.1  E3 ubiquitin-protein ligase RNF185 isoform 2

    See identical proteins and their annotated locations for NP_001129297.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) is lacking two in-frame coding exons compared to transcript variant 1, resulting in a shorter isoform (2) missing a 56 aa protein segment compared to isoform 1.
    Source sequence(s)
    AA258379, DB134817, DQ296562
    Consensus CDS
    CCDS46689.1
    Related
    ENSP00000266252.7, ENST00000266252.8
    Conserved Domains (1) summary
    cl17238
    Location:37 → 64
    RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain

  2. NM_152267.4 → NP_689480.2  E3 ubiquitin-protein ligase RNF185 isoform 1

    See identical proteins and their annotated locations for NP_689480.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (1).
    Source sequence(s)
    AA258379, BC033166, DB134817
    Consensus CDS
    CCDS13890.1
    UniProtKB/Swiss-Prot
    A8K5C1, A9X3T8, Q8N900, Q96GF1
    Related
    ENSP00000320508.5, ENST00000326132.11
    Conserved Domains (1) summary
    cd16744
    Location:38 → 80
    RING-HC_RNF185; RING finger, HC subclass, found in RING finger protein 185 (RNF185) and similar proteins

RNA

  1. NR_024209.2 RNA Sequence

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) is missing an internal coding exon and contains an additional exon compared to transcript variant 1. This results in a frame-shift and premature translation termination, rendering the transcript susceptible to nonsense mediated mRNA decay (NMD). This transcript is sufficiently abundant to represent as a RefSeq record, however, the predicted protein is not represented because it is significantly truncated.
    Source sequence(s)
    AA258379, BC033166, BX648019, DB134817

  2. NR_024210.2 RNA Sequence

    Status: VALIDATED

    Description
    Transcript Variant: This variant (5) uses an alternate donor splice site at one of the coding exons compared to transcript variant 1. This results in a frame-shift and premature translation termination, rendering the transcript susceptible to nonsense mediated mRNA decay (NMD). This transcript is sufficiently abundant to represent as a RefSeq record, however, the predicted protein is not represented because it is truncated.
    Source sequence(s)
    AA258379, BC033166, DA498404, DB134817

  3. NR_024211.2 RNA Sequence

    Status: VALIDATED

    Description
    Transcript Variant: This variant (6) is missing an internal coding exon compared to transcript variant 1. This results in a frame-shift and premature translation termination, rendering the transcript susceptible to nonsense mediated mRNA decay (NMD). This transcript is sufficiently abundant to represent as a RefSeq record, however, the predicted protein is not represented because it is significantly truncated.
    Source sequence(s)
    AA258379, BC033166, BX648019, DB134817

  4. NR_024212.2 RNA Sequence

    Status: VALIDATED

    Description
    Transcript Variant: This variant (7) is missing a coding exon (containing the translation start site) compared to transcript variant 1. This results in the use of a downstream AUG in a different frame, and a predicted protein that is severely truncated with no similarity to this gene product. However, this transcript is sufficiently abundant to represent as a RefSeq record.
    Source sequence(s)
    AA258379, BC033166, DB134817
    Related
    ENST00000471384.5

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000022.11 Reference GRCh38.p14 Primary Assembly

    Range
    31160182..31207019
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060946.1 Alternate T2T-CHM13v2.0

    Range
    31623670..31670571
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_001135824.1: Suppressed sequence

    Description
    NM_001135824.1: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript.
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q96GF1.1 GenPept UniProtKB/Swiss-Prot:Q96GF1

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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