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pknG serine/threonine-protein kinase PknG [ Mycobacterium tuberculosis H37Rv ]

Gene ID: 886397, updated on 6-Aug-2024

Summary

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Gene symbol
pknG
Gene description
serine/threonine-protein kinase PknG
Locus tag
Rv0410c
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Mycobacterium tuberculosis H37Rv (strain: H37Rv, type-material: type strain of Mycobacterium tuberculosis)
Lineage
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex
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Genomic context

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Sequence:
NC_000962.3 (495062..497314, complement)

NC_000962.3Genomic Context describing neighboring genes Neighboring gene F420-dependent glucose-6-phosphate dehydrogenase Neighboring gene phosphate acetyltransferase Neighboring gene acetate kinase Neighboring gene glutamine-binding lipoprotein GlnH Neighboring gene membrane protein Neighboring gene 8-oxo-dGTP diphosphatase Neighboring gene thiamine-phosphate synthase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000962.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. New substrates and interactors of the mycobacterial Serine/Threonine protein kinase PknG identified by a tailored interactomic approach. Gil M, et al. J Proteomics, 2019 Feb 10. PMID 30267874
  2. PknG supports mycobacterial adaptation in acidic environment. Paroha R, et al. Mol Cell Biochem, 2018 Jun. PMID 29124568
  3. Sclerotiorin inhibits protein kinase G from Mycobacterium tuberculosis and impairs mycobacterial growth in macrophages. Chen D, et al. Tuberculosis (Edinb), 2017 Mar. PMID 28237032
  4. Molecular Basis of the Activity and the Regulation of the Eukaryotic-like S/T Protein Kinase PknG from Mycobacterium tuberculosis. Lisa MN, et al. Structure, 2015 Jun 2. PMID 25960409
  5. Inhibition of phagosome maturation by mycobacteria does not interfere with presentation of mycobacterial antigens by MHC molecules. Majlessi L, et al. J Immunol, 2007 Aug 1. PMID 17641049

See all (15) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. results show that PknG phosphorylates specific residues in both glutamine synthetase and FhaA in vitro, and suggest that these proteins are phosphorylated by PknG in living mycobacteria.
    Title: New substrates and interactors of the mycobacterial Serine/Threonine protein kinase PknG identified by a tailored interactomic approach.
  2. data strongly suggests that pknG regulates growth of mycobacteria in acidic environment and is differentially transcribed in MS and Mtb under these conditions.
    Title: PknG supports mycobacterial adaptation in acidic environment.
  3. Sclerotiorin has moderately strong PknG inhibitory activity, but no cytotoxicity, and it could substantially decrease the growth of mycobacterium tuberculosis inside macrophages.
    Title: Sclerotiorin inhibits protein kinase G from Mycobacterium tuberculosis and impairs mycobacterial growth in macrophages.
  4. Data suggest PknG is necessary in Mycobacterium tuberculosis for efficient metabolic adaptation during stress such as exposure to antibiotics and hypoxic environment; PknG-mediated GarA phosphorylation is important for maintenance of both mycobacterial physiology and redox poise. (PknG = protein kinase G; GarA = glycogen accumulation regulator)
    Title: Protein kinase G confers survival advantage to Mycobacterium tuberculosis during latency-like conditions.
  5. GarA is a cellular target of PknG and the metabolomics data demonstrate that the function of this signaling system is in metabolic regulation.
    Title: PknG senses amino acid availability to control metabolism and virulence of Mycobacterium tuberculosis.
  6. Different motifs and domains flanking the catalytic core regulate substrate selectivity without significantly affecting the intrinsic kinase activity.
    Title: Molecular Basis of the Activity and the Regulation of the Eukaryotic-like S/T Protein Kinase PknG from Mycobacterium tuberculosis.
  7. Thereby, the established preparation protocols pave the route for the NMR structural characterization of PknG regulation by its N-terminal regions, which is demonstrated by the recorded initial (1)H-(15)N-HSQC spectra.
    Title: Expression and purification of the natively disordered and redox sensitive metal binding regions of Mycobacterium tuberculosis protein kinase G.
  8. The results demonstrate that pathogenic mycobacteria recognize and then inhibit PKCalpha via the action of PknG to circumvent phagocytosis and the hostile environment of macrophages.
    Title: Downregulation of protein kinase C-alpha enhances intracellular survival of Mycobacteria: role of PknG.
  9. Autophosphorylation was crucial for the capacity of PknG to promote mycobacterial survival within macrophages.
    Title: Survival of pathogenic mycobacteria in macrophages is mediated through autophosphorylation of protein kinase G.
  10. Presentation of mycobacterial antigen PknG is independent of the site of its intracellular residence inside professional antigen presenting cells.
    Title: Inhibition of phagosome maturation by mycobacteria does not interfere with presentation of mycobacterial antigens by MHC molecules.
Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

General protein information

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Preferred Names
serine/threonine-protein kinase PknG

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000962.3 Reference assembly

    Range
    495062..497314 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_214924.1 serine/threonine-protein kinase PknG [Mycobacterium tuberculosis H37Rv]

    See identical proteins and their annotated locations for NP_214924.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A0U0RG23
    Conserved Domains (3) summary
    cd14014
    Location:150 → 394
    STKc_PknB_like; Catalytic domain of bacterial Serine/Threonine kinases, PknB and similar proteins
    pfam16918
    Location:409 → 748
    PknG_TPR; Protein kinase G tetratricopeptide repeat
    pfam16919
    Location:12 → 150
    PknG_rubred; Protein kinase G rubredoxin domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P9WI73.1

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