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waaL O-antigen ligase WaaL [ Pseudomonas aeruginosa PAO1 ]

Gene ID: 881661, updated on 24-Aug-2024

Summary

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Gene symbol
waaL
Gene description
O-antigen ligase WaaL
Locus tag
PA4999
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Pseudomonas aeruginosa PAO1 (strain: PAO1)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
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Genomic context

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Sequence:
NC_002516.2 (5616310..5617515)

NC_002516.2Genomic Context describing neighboring genes Neighboring gene transporter MsbA Neighboring gene hypothetical protein Neighboring gene alpha-1,3-rhamnosyltransferase WapR Neighboring gene hypothetical protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_002516.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Both E. coli and P. aeruginosa WaaL proteins share similar membrane topology. P. aeruginosa WaaL shares similar functional properties with E. coli WaaL and E. coli WaaL may adopt a functional dimer conformation.
    Title: Escherichia coli and Pseudomonas aeruginosa lipopolysaccharide O-antigen ligases share similar membrane topology and biochemical properties.
  2. use of the waaLmotABmotCD mutant revealed that loss of motility confers resistance to phagocytosis regardless of the smooth or rough LPS phenotype. These findings reveal how the O-antigen of P. aeruginosa can influence bacterial clearance during infection and expand our current knowledge about the impact of bacterial phenotypic changes during chronic infection.
    Title: The effect of loss of O-antigen ligase on phagocytic susceptibility of motile and non-motile Pseudomonas aeruginosa.
  3. The WaaL O-antigen lipopolysaccharide ligase has features in common with metal ion-independent inverting glycosyltransferases.
    Title: The WaaL O-antigen lipopolysaccharide ligase has features in common with metal ion-independent inverting glycosyltransferases.
  4. WaaL is an integral inner membrane protein with 12 transmembrane domains.
    Title: Membrane topology mapping of the O-antigen flippase (Wzx), polymerase (Wzy), and ligase (WaaL) from Pseudomonas aeruginosa PAO1 reveals novel domain architectures.
  5. Data show that WaaL of Pseudomonas aeruginosa utilizes ATP in in vitro ligation of O antigen onto lipid A-core.
    Title: WaaL of Pseudomonas aeruginosa utilizes ATP in in vitro ligation of O antigen onto lipid A-core.
  6. These results demonstrate that waaL in P. aeruginosa encodes a functional O-antigen ligase that is important for cell wall integrity and motility of the bacteria.
    Title: Functional characterization of WaaL, a ligase associated with linking O-antigen polysaccharide to the core of Pseudomonas aeruginosa lipopolysaccharide.
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General gene information

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Homology

Gene Ontology Provided by PseudoCAP

Function Evidence Code Pubs
enables O antigen ligase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables glycosyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within O antigen biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within bacterial-type flagellum assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within bacterial-type flagellum-dependent swimming motility IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within pilus assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within type IV pilus-dependent motility IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in periplasmic side of plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
O-antigen ligase WaaL
NP_253686.1
  • Product name confidence: class 1 (Function experimentally demonstrated in P. aeruginosa)

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_002516.2 Reference assembly

    Range
    5616310..5617515
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_253686.1 O-antigen ligase WaaL [Pseudomonas aeruginosa PAO1]

    See identical proteins and their annotated locations for NP_253686.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A367MHL7
    Conserved Domains (1) summary
    COG3307
    Location:1 → 401
    RfaL; O-antigen ligase [Cell wall/membrane/envelope biogenesis]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9HUG6.1

Gene LinkOut

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Molecular Biology Databases