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rhlR transcriptional regulator RhlR [ Pseudomonas aeruginosa PAO1 ]

Gene ID: 878968, updated on 6-Oct-2023

Summary

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Gene symbol
rhlR
Gene description
transcriptional regulator RhlR
Locus tag
PA3477
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Pseudomonas aeruginosa PAO1 (strain: PAO1)
Lineage
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
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Genomic context

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Sequence:
NC_002516.2 (3889925..3890650, complement)

NC_002516.2Genomic Context describing neighboring genes Neighboring gene cyclohexadienyl dehydratase Neighboring gene acyl-homoserine-lactone synthase Neighboring gene rhamnosyltransferase subunit B Neighboring gene rhamnosyltransferase subunit A

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_002516.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Identification of ligand binding activity and DNA recognition by RhlR protein from opportunistic pathogen Pseudomonas aeruginosa-a molecular dynamic simulation approach. Chowdhury N, et al. J Mol Recognit, 2018 Dec. PMID 29968257
  2. Refolding, purification, and preliminary structural characterization of the DNA-binding domain of the quorum sensing receptor RhlR from Pseudomonas aeruginosa. Lixa C, et al. Protein Expr Purif, 2016 May. PMID 26792557
  3. Inactivation of the quorum-sensing transcriptional regulators LasR or RhlR does not suppress the expression of virulence factors and the virulence of Pseudomonas aeruginosa PAO1. Soto-Aceves MP, et al. Microbiology (Reading), 2019 Apr. PMID 30707095
  4. Quorum sensing in Pseudomonas aeruginosa mediated by RhlR is regulated by a small RNA PhrD. Malgaonkar A, et al. Sci Rep, 2019 Jan 23. PMID 30674910, Free PMC Article
  5. The PqsE and RhlR proteins are an autoinducer synthase-receptor pair that control virulence and biofilm development in Pseudomonas aeruginosa. Mukherjee S, et al. Proc Natl Acad Sci U S A, 2018 Oct 2. PMID 30224496, Free PMC Article

See all (28) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The interaction of RhlR-5'UTR with PhrD in E. coli indicated that this regulation could function without the involvement of any Pseudomonas specific proteins.
    Title: Quorum sensing in Pseudomonas aeruginosa mediated by RhlR is regulated by a small RNA PhrD.
  2. Inactivation of RhlR protein (rhlR) of Pseudomonas aeruginosa does not block the quorum-sensing (QSR), nor impairs the virulence, and that even a LasR protein (lasR) rhlR double mutant presents residual virulence, even lacking the production of virulence factors. These results show that the inhibition of either lasR or rhlR is not a straightforward approach to blocking virulence, due to the great complexity of the QSR.
    Title: Inactivation of the quorum-sensing transcriptional regulators LasR or RhlR does not suppress the expression of virulence factors and the virulence of Pseudomonas aeruginosa PAO1.
  3. Study identifies the mechanism of the binding of the autoinducer BHL ligand to the dimeric RhlR protein, which would further help the dimeric RhlR protein to bind the cognate promoter DNA to initiate the transcription of virulence genes. Also, the key amino acid residues from the RhlR protein were identified to be involved in ligand binding, protein-protein interactions, and DNA binding.
    Title: Identification of ligand binding activity and DNA recognition by RhlR protein from opportunistic pathogen Pseudomonas aeruginosa-a molecular dynamic simulation approach.
  4. rhlI mutants are unexpectedly more virulent than rhlR mutants, both in fly and in nematode intestinal infection models, suggesting that RhlR has RhlI-independent functions
    Title: Quorum-sensing regulator RhlR but not its autoinducer RhlI enables Pseudomonas to evade opsonization.
  5. this work shows that PqsE and RhlR function as a QS-autoinducer synthase-receptor pair that drives group behaviors in P. aeruginosa.
    Title: The PqsE and RhlR proteins are an autoinducer synthase-receptor pair that control virulence and biofilm development in Pseudomonas aeruginosa.
  6. rhlR/lasR knockout increased the susceptibility of Pseudomonas aeruginosa towards polymyxin B-azithromycin combination via influencing quorum sensing.
    Title: Influence of rhlR and lasR on Polymyxin Pharmacodynamics in Pseudomonas aeruginosa and Implications for Quorum Sensing Inhibition with Azithromycin.
  7. This is the first time that a domain of the quorum sensing regulator RhlR was produced in sufficient amounts for structural studies, enabling the investigation of the molecular basis for RhlR specific interaction with DNA promoters.
    Title: Refolding, purification, and preliminary structural characterization of the DNA-binding domain of the quorum sensing receptor RhlR from Pseudomonas aeruginosa.
  8. Quorum sensing genes lasR and rhlR and activity of electric field sensitive enzyme, glycerol-3-phosphate dehydrogenase was also repressed by WED.
    Title: Silver-zinc redox-coupled electroceutical wound dressing disrupts bacterial biofilm.
  9. Subinhibitory concentrations of macrolides specifically upregulated expression of quorum sensing genes (rhlR, lasI), whereas sulfonamides and municipal wastewater, instead upregulated expression of specific resistant genes (sul1) and efflux pumps (mexD).
    Title: Sub-inhibitory concentrations of antibiotics and wastewater influencing biofilm formation and gene expression of multi-resistant Pseudomonas aeruginosa wastewater isolates.
  10. The rmlBDAC operon has three promoters, and that the expression of the P2 promoter is dependent on the stationary phase sigma factor sigmaS and on the quorum sensing regulator RhlR.
    Title: The Pseudomonas aeruginosa rmlBDAC operon, encoding dTDP-L-rhamnose biosynthetic enzymes, is regulated by the quorum-sensing transcriptional regulator RhlR and the alternative sigma factor σS.
Submit: New GeneRIF Correction See all GeneRIFs (17)

General gene information

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Homology

Gene Ontology Provided by PseudoCAP

Process Evidence Code Pubs
acts_upstream_of_or_within positive regulation of lipid biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within positive regulation of proteolysis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within positive regulation of secondary metabolite biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 

General protein information

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Preferred Names
transcriptional regulator RhlR
NP_252167.1
  • Product name confidence: class 1 (Function experimentally demonstrated in P. aeruginosa)

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_002516.2 Reference assembly

    Range
    3889925..3890650 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_252167.1 transcriptional regulator RhlR [Pseudomonas aeruginosa PAO1]

    See identical proteins and their annotated locations for NP_252167.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A411F6T9, B6E4Z5
    Conserved Domains (1) summary
    PRK10188
    Location:4 → 241
    PRK10188; transcriptional regulator SdiA
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P54292.1

Gene LinkOut

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Molecular Biology Databases
Research Materials