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lasA protease LasA [ Pseudomonas aeruginosa PAO1 ]

Gene ID: 878260, updated on 11-Sep-2024

Summary

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Gene symbol
lasA
Gene description
protease LasA
Locus tag
PA1871
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Pseudomonas aeruginosa PAO1 (strain: PAO1)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas
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Genomic context

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Sequence:
NC_002516.2 (2032695..2033951)

NC_002516.2Genomic Context describing neighboring genes Neighboring gene acyl carrier protein Neighboring gene hypothetical protein Neighboring gene hypothetical protein Neighboring gene hypothetical protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_002516.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Crystal structure of the LasA virulence factor from Pseudomonas aeruginosa: substrate specificity and mechanism of M23 metallopeptidases. Spencer J, et al. J Mol Biol, 2010 Mar 5. PMID 20026068
  2. Identification of critical residues in the propeptide of LasA protease of Pseudomonas aeruginosa involved in the formation of a stable mature protease. Grande KK, et al. J Bacteriol, 2007 Jun. PMID 17351039, Free PMC Article
  3. Quorum sensing-dependent post-secretional activation of extracellular proteases in Pseudomonas aeruginosa. Li XH, et al. J Biol Chem, 2019 Dec 20. PMID 31727738, Free PMC Article
  4. Clearance of Pseudomonas aeruginosa from a healthy ocular surface involves surfactant protein D and is compromised by bacterial elastase in a murine null-infection model. Mun JJ, et al. Infect Immun, 2009 Jun. PMID 19349424, Free PMC Article
  5. Pseudomonas Genome Database: facilitating user-friendly, comprehensive comparisons of microbial genomes. Winsor GL, et al. Nucleic Acids Res, 2009 Jan. PMID 18978025, Free PMC Article

See all (14) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. LasB, PIV, and LasA are activated postsecretionally in a cascading manner in which the initial activation of LasB was controlled tightly by quorum sensing (QS) at the protein level in addition to the well-known transcriptional control of these proteases by QS
    Title: Quorum sensing-dependent post-secretional activation of extracellular proteases in Pseudomonas aeruginosa.
  2. crystal structures of active LasA as a complex with tartrate and in the uncomplexed form. While the overall fold resembles that of the other M23 family members, the LasA active site is less constricted and utilizes a different set of metal ligands.
    Title: Crystal structure of the LasA virulence factor from Pseudomonas aeruginosa: substrate specificity and mechanism of M23 metallopeptidases.
  3. These data show that SP-D can contribute to the clearance of P. aeruginosa from the healthy ocular surface and that proteases can compromise that clearance.
    Title: Clearance of Pseudomonas aeruginosa from a healthy ocular surface involves surfactant protein D and is compromised by bacterial elastase in a murine null-infection model.
  4. Data suggest that these clustered residues in the propeptide may play an important role in promoting the correct protein conformation of the mature LasA protease domain.
    Title: Identification of critical residues in the propeptide of LasA protease of Pseudomonas aeruginosa involved in the formation of a stable mature protease.
Submit: New GeneRIF Correction

General gene information

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Homology

Gene Ontology Provided by PseudoCAP

Function Evidence Code Pubs
enables endopeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within protein secretion by the type II secretion system IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within protein transport by the Sec complex IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within proteolysis IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in extracellular space IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
protease LasA
NP_250562.1
  • Product name confidence: class 1 (Function experimentally demonstrated in P. aeruginosa)

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_002516.2 Reference assembly

    Range
    2032695..2033951
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_250562.1 protease LasA [Pseudomonas aeruginosa PAO1]

    See identical proteins and their annotated locations for NP_250562.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A367M654
    Conserved Domains (2) summary
    COG0739
    Location:187 → 369
    NlpD; Murein DD-endopeptidase MepM and murein hydrolase activator NlpD, contain LysM domain [Cell wall/membrane/envelope biogenesis]
    cd12797
    Location:287 → 361
    M23_peptidase; M23 family metallopeptidase, also known as beta-lytic metallopeptidase, and similar proteins
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P14789.3

Gene LinkOut

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Molecular Biology Databases
Research Materials