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EIF3J eukaryotic translation initiation factor 3 subunit J [ Homo sapiens (human) ]

Gene ID: 8669, updated on 5-Mar-2024

Summary

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Official Symbol
EIF3Jprovided by HGNC
Official Full Name
eukaryotic translation initiation factor 3 subunit Jprovided by HGNC
Primary source
HGNC:HGNC:3270
See related
Ensembl:ENSG00000104131 MIM:603910; AllianceGenome:HGNC:3270
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
EIF3S1; eIF3-p35; eIF3-alpha
Summary
This gene encodes a core subunit of the eukaryotic initiation factor 3 complex, which participates in the initiation of translation by aiding in the recruitment of protein and mRNA components to the 40S ribosome. There are pseudogenes for this gene on chromosomes 1, 3, and 9. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2013]
Expression
Ubiquitous expression in appendix (RPKM 31.0), brain (RPKM 29.6) and 25 other tissues See more
Orthologs
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Genomic context

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See EIF3J in Genome Data Viewer
Location:
15q21.1
Exon count:
8
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 15 NC_000015.10 (44537147..44562803)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 15 NC_060939.1 (42345115..42370769)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 15 NC_000015.9 (44829345..44855001)

Chromosome 15 - NC_000015.10Genomic Context describing neighboring genes Neighboring gene CTD small phosphatase like 2 Neighboring gene heterogeneous nuclear ribonucleoprotein M pseudogene 1 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr15:44828013-44828800 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6395 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6396 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6397 Neighboring gene EIF3J divergent transcript Neighboring gene ReSE screen-validated silencer GRCh37_chr15:44874604-44874775 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6398 Neighboring gene ReSE screen-validated silencer GRCh37_chr15:44879793-44880004 Neighboring gene SPG11 vesicle trafficking associated, spatacsin Neighboring gene ATAC-STARR-seq lymphoblastoid active region 9340 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 9341 Neighboring gene ReSE screen-validated silencer GRCh37_chr15:44936676-44936780 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 9342 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr15:44955204-44956160 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 9344 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 9345 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 9346 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 9347 Neighboring gene PAT1 homolog 2 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr15:45003536-45004198 Neighboring gene microRNA 10393 Neighboring gene beta-2-microglobulin

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. eIF3j facilitates loading of release factors into the ribosome. Egorova T, et al. Nucleic Acids Res, 2021 Nov 8. PMID 34591963, Free PMC Article
  2. Association between eIF3α polymorphism and severe toxicity caused by platinum-based chemotherapy in non-small cell lung cancer patients. Xu X, et al. Br J Clin Pharmacol, 2013 Feb. PMID 22804784, Free PMC Article
  3. All translation elongation factors and the e, f, and h subunits of translation initiation factor 3 are encoded by 5'-terminal oligopyrimidine (TOP) mRNAs. Iadevaia V, et al. RNA, 2008 Sep. PMID 18658124, Free PMC Article
  4. eIF3j is located in the decoding center of the human 40S ribosomal subunit. Fraser CS, et al. Mol Cell, 2007 Jun 22. PMID 17588516
  5. Protein kinase CK2 potentiates translation efficiency by phosphorylating eIF3j at Ser127. Borgo C, et al. Biochim Biophys Acta, 2015 Jul. PMID 25887626

See all (87) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. eIF3a sustains non-small cell lung cancer stem cell-like properties by promoting YY1-mediated transcriptional activation of beta-catenin.
    Title: eIF3a sustains non-small cell lung cancer stem cell-like properties by promoting YY1-mediated transcriptional activation of β-catenin.
  2. eIF3j facilitates loading of release factors into the ribosome.
    Title: eIF3j facilitates loading of release factors into the ribosome.
  3. CK2-phosphorylation of eIF3j at Ser127 promotes the assembly of the eIF3 complex, a crucial step in the activation of the translation initiation machinery.
    Title: Protein kinase CK2 potentiates translation efficiency by phosphorylating eIF3j at Ser127.
  4. eIF1, eIF1A, eIF3j, and the eIF2-GTP-Met-tRNAi ternary complex stably bind to the 43 S preinitiation complex
    Title: Human eukaryotic initiation factor 2 (eIF2)-GTP-Met-tRNAi ternary complex and eIF3 stabilize the 43 S preinitiation complex.
  5. whether eIF3alpha polymorphism is associated with severe platinum toxicity in patients with non-small cell lung cancer (NSCLC).
    Title: Association between eIF3α polymorphism and severe toxicity caused by platinum-based chemotherapy in non-small cell lung cancer patients.
  6. Beta-Arrestin 2 modulates binding of eIF-3-alpha to selected components of the extracellular signal-regulated kinase pathway.
    Title: Eukaryotic translation initiation factor 3, subunit a, regulates the extracellular signal-regulated kinase pathway.
  7. a conserved tryptophan residue in the human eIF3j N-terminal acidic motif (NTA) is held in the helix alpha1 and loop 5 hydrophobic pocket of the human eIF3b RNA recognition motif (RRM)
    Title: The indispensable N-terminal half of eIF3j/HCR1 cooperates with its structurally conserved binding partner eIF3b/PRT1-RRM and with eIF1A in stringent AUG selection.
  8. structural analysis of the eIF3b RNA recognition motif and its interaction with eIF3j
    Title: Structure of eIF3b RNA recognition motif and its interaction with eIF3j: structural insights into the recruitment of eIF3b to the 40 S ribosomal subunit.
Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
contributes_to translation initiation factor activity IC
Inferred by Curator
more info
 PubMed 
Process Evidence Code Pubs
involved_in formation of cytoplasmic translation initiation complex NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in translational initiation IC
Inferred by Curator
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
  
located_in cytosol TAS
Traceable Author Statement
more info
  
part_of eukaryotic 43S preinitiation complex IEA
Inferred from Electronic Annotation
more info
  
part_of eukaryotic 48S preinitiation complex IEA
Inferred from Electronic Annotation
more info
  
part_of eukaryotic translation initiation factor 3 complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of eukaryotic translation initiation factor 3 complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of eukaryotic translation initiation factor 3 complex IPI
Inferred from Physical Interaction
more info
 PubMed 

General protein information

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Preferred Names
eukaryotic translation initiation factor 3 subunit J
Names
eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD)

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001284335.2 → NP_001271264.1  eukaryotic translation initiation factor 3 subunit J isoform 2

    See identical proteins and their annotated locations for NP_001271264.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
    Source sequence(s)
    AC009996, AK300659, BC002719, BU621047
    Consensus CDS
    CCDS61612.1
    UniProtKB/Swiss-Prot
    O75822
    Related
    ENSP00000440221.1, ENST00000535391.5
    Conserved Domains (1) summary
    pfam08597
    Location:12 → 136
    eIF3_subunit; Translation initiation factor eIF3 subunit

  2. NM_001284336.2 → NP_001271265.1  eukaryotic translation initiation factor 3 subunit J isoform 3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) lacks two alternate in-frame exons, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.
    Source sequence(s)
    AC009996, AL536561, BC002719, BU621047
    Consensus CDS
    CCDS61613.1
    UniProtKB/Swiss-Prot
    O75822
    Related
    ENSP00000414548.3, ENST00000424492.7
    Conserved Domains (1) summary
    pfam08597
    Location:12 → 209
    eIF3_subunit; Translation initiation factor eIF3 subunit

  3. NM_003758.4 → NP_003749.2  eukaryotic translation initiation factor 3 subunit J isoform 1

    See identical proteins and their annotated locations for NP_003749.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
    Source sequence(s)
    AC009996, BC002719, BU621047
    Consensus CDS
    CCDS10111.1
    UniProtKB/Swiss-Prot
    B4DUI3, F5H425, O75822, Q9BUD2, Q9H8Q2, Q9UI65
    Related
    ENSP00000261868.5, ENST00000261868.10
    Conserved Domains (1) summary
    pfam08597
    Location:12 → 258
    eIF3_subunit; Translation initiation factor eIF3 subunit

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000015.10 Reference GRCh38.p14 Primary Assembly

    Range
    44537147..44562803
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060939.1 Alternate T2T-CHM13v2.0

    Range
    42345115..42370769
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O75822.2 GenPept UniProtKB/Swiss-Prot:O75822

Gene LinkOut

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