USO1 USO1 vesicle transport factor [Homo sapiens (human)] - Gene

Summary

Official Symbol: USO1provided by HGNC
Official Full Name: USO1 vesicle transport factorprovided by HGNC
Primary source: HGNC:HGNC:30904
See related: Ensembl:ENSG00000138768 MIM:603344; AllianceGenome:HGNC:30904
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: TAP; VDP; P115
Summary: The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Expression: Ubiquitous expression in thyroid (RPKM 38.1), testis (RPKM 30.9) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 4q21.1

Exon count:: 26

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	4	NC_000004.12 (75724577..75814286)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	4	NC_060928.1 (79064705..79154419)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	4	NC_000004.11 (76649761..76735439)

Chromosome 4 - NC_000004.12 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

USO1 isoforms differentially promote liver cancer progression by dysregulating the ER-Golgi network.
Title: USO1 isoforms differentially promote liver cancer progression by dysregulating the ER-Golgi network.
Focused CRISPR-Cas9 genetic screening reveals USO1 as a vulnerability in B-cell acute lymphoblastic leukemia.
Title: Focused CRISPR-Cas9 genetic screening reveals USO1 as a vulnerability in B-cell acute lymphoblastic leukemia.
USO1 gene may be a promising target for the therapy of human multiple myeloma and its diagnosis marker.
Title: USO1 promotes tumor progression via activating Erk pathway in multiple myeloma cells.
USO1 gene has a critical role in human colon cancer: USO1 is involved in cell proliferation and cell migration of colon cancer cells.
Title: Lentivirus-mediated silencing of USO1 inhibits cell proliferation and migration of human colon cancer cells.
P115 promotes proliferation of gastric cancer cells through an interaction with MIF.
Title: P115 promotes growth of gastric cancer through interaction with macrophage migration inhibitory factor.
Scyl1 interacts with 58K/formiminotransferase cyclodeaminase (FTCD) and golgin p115, and is required for the maintenance of Golgi morphology.
Title: Scyl1 regulates Golgi morphology.
p115 plays a critical role in mitosis progression, implicating it as the only known golgin to regulate both mitosis and apoptosis
Title: The Golgi protein p115 associates with gamma-tubulin and plays a role in Golgi structure and mitosis progression.
a conserved tethering function of the Golgi protein p115 CTF which promotes p53-ERK interaction for the amplification of the apoptotic signal.
Title: Tethering function of the caspase cleavage fragment of Golgi protein p115 promotes apoptosis via a p53-dependent pathway.
Data show that the number of tandem ARM repeats in an ARM fold ranges from 6 to 12.
Title: Armadillo motifs involved in vesicular transport.
p115 is a critical intermediary component in the regulated secretion of MIF from monocytes/macrophages
Title: The Golgi-associated protein p115 mediates the secretion of macrophage migration inhibitory factor.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Vpr	vpr	A stable-isotope labeling by amino acids in cell culture coupled with mass spectrometry-based proteomics identifies upregulation of vesicle transport factor p115 (USO1) expression by HIV-1 Vpr in Vpr transduced macrophages	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

REN93022 (e-PCR)
- UniSTS:417820

REN93023 (e-PCR)
- UniSTS:417821

RH46891 (e-PCR)
- UniSTS:2380

SHGC4-1677 (e-PCR)
- UniSTS:28495

G19677 (e-PCR)
- UniSTS:77230

A001V03 (e-PCR)
- UniSTS:77231

SHGC-36721 (e-PCR)
- UniSTS:2813

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables RNA binding	HDA more info	PubMed
enables cadherin binding	HDA more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Golgi vesicle docking	IBA Inferred from Biological aspect of Ancestor more info
involved_in endoplasmic reticulum to Golgi vesicle-mediated transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in endoplasmic reticulum to Golgi vesicle-mediated transport	ISS Inferred from Sequence or Structural Similarity more info
involved_in intracellular protein transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in membrane fusion	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of cellular response to insulin stimulus	IEA Inferred from Electronic Annotation more info
involved_in secretory granule localization	IEA Inferred from Electronic Annotation more info
involved_in small GTPase-mediated signal transduction	IEA Inferred from Electronic Annotation more info
involved_in transcytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in vesicle fusion with Golgi apparatus	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in ER to Golgi transport vesicle membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in ER to Golgi transport vesicle membrane	TAS Traceable Author Statement more info
located_in Golgi apparatus	IDA Inferred from Direct Assay more info
located_in Golgi membrane	TAS Traceable Author Statement more info
is_active_in Golgi stack	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	TAS Traceable Author Statement more info
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in fibrillar center	IDA Inferred from Direct Assay more info
located_in membrane	HDA more info	PubMed
located_in microtubule organizing center	TAS Traceable Author Statement more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
located_in transport vesicle	TAS Traceable Author Statement more info

General protein information

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Preferred Names: general vesicular transport factor p115

Names: USO1 vesicle docking protein homolog; transcytosis associated protein; vesicle docking protein p115

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001290049.2 → NP_001276978.1 general vesicular transport factor p115 isoform 1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC104828, AC110615, AEKP01043041

Consensus CDS

CCDS77929.1

UniProtKB/Swiss-Prot

O60763

Related

ENSP00000264904.7, ENST00000264904.8

Conserved Domains (5) summary

cd00020
Location:19 → 92

ARM; Armadillo/beta-catenin-like repeats. An approximately 40 amino acid long tandemly repeated sequence motif first identified in the Drosophila segment polarity gene armadillo; these repeats were also found in the mammalian armadillo homolog beta-catenin, ...

pfam04869
Location:351 → 639

Uso1_p115_head; Uso1 / p115 like vesicle tethering protein, head region

pfam04871
Location:837 → 947

Uso1_p115_C; Uso1 / p115 like vesicle tethering protein, C terminal region

pfam15619
Location:672 → 876

Lebercilin; Ciliary protein causing Leber congenital amaurosis disease

cl19219
Location:746 → 877

DUF342; Protein of unknown function (DUF342)
NM_003715.4 → NP_003706.2 general vesicular transport factor p115 isoform 2

See identical proteins and their annotated locations for NP_003706.2

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks two exons in the central coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.

Source sequence(s)

AC104828, AC110615, AEKP01043041

Consensus CDS

CCDS75144.1

UniProtKB/Swiss-Prot

B2RAQ0, O60763, Q6PK63, Q86TB8, Q8N592

Related

ENSP00000444850.2, ENST00000514213.7

Conserved Domains (5) summary

cd00020
Location:19 → 153

ARM; Armadillo/beta-catenin-like repeats. An approximately 40 amino acid long tandemly repeated sequence motif first identified in the Drosophila segment polarity gene armadillo; these repeats were also found in the mammalian armadillo homolog beta-catenin, ...

pfam04869
Location:347 → 628

Uso1_p115_head; Uso1 / p115 like vesicle tethering protein, head region

pfam04871
Location:826 → 936

Uso1_p115_C; Uso1 / p115 like vesicle tethering protein, C terminal region

pfam15619
Location:661 → 865

Lebercilin; Ciliary protein causing Leber congenital amaurosis disease

cl19219
Location:735 → 866

DUF342; Protein of unknown function (DUF342)

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000004.12 Reference GRCh38.p14 Primary Assembly

Range

75724577..75814286

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006714397.4 → XP_006714460.1 general vesicular transport factor p115 isoform X2

Conserved Domains (5) summary

cd00020
Location:19 → 92

ARM; Armadillo/beta-catenin-like repeats. An approximately 40 amino acid long tandemly repeated sequence motif first identified in the Drosophila segment polarity gene armadillo; these repeats were also found in the mammalian armadillo homolog beta-catenin, ...

pfam04869
Location:351 → 632

Uso1_p115_head; Uso1 / p115 like vesicle tethering protein, head region

pfam04871
Location:830 → 940

Uso1_p115_C; Uso1 / p115 like vesicle tethering protein, C terminal region

pfam15619
Location:665 → 869

Lebercilin; Ciliary protein causing Leber congenital amaurosis disease

cl19219
Location:739 → 870

DUF342; Protein of unknown function (DUF342)
XM_006714396.5 → XP_006714459.1 general vesicular transport factor p115 isoform X1

Conserved Domains (5) summary

cd00020
Location:19 → 153

ARM; Armadillo/beta-catenin-like repeats. An approximately 40 amino acid long tandemly repeated sequence motif first identified in the Drosophila segment polarity gene armadillo; these repeats were also found in the mammalian armadillo homolog beta-catenin, ...

pfam04869
Location:347 → 635

Uso1_p115_head; Uso1 / p115 like vesicle tethering protein, head region

pfam04871
Location:833 → 943

Uso1_p115_C; Uso1 / p115 like vesicle tethering protein, C terminal region

pfam15619
Location:668 → 872

Lebercilin; Ciliary protein causing Leber congenital amaurosis disease

cl19219
Location:742 → 873

DUF342; Protein of unknown function (DUF342)

Alternate T2T-CHM13v2.0

Genomic

NC_060928.1 Alternate T2T-CHM13v2.0

Range

79064705..79154419

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_054351146.1 → XP_054207121.1 general vesicular transport factor p115 isoform X2
XM_054351145.1 → XP_054207120.1 general vesicular transport factor p115 isoform X1