IRE1 bifunctional endoribonuclease/protein kinase IRE1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: IRE1
Official Full Name: bifunctional endoribonuclease/protein kinase IRE1
Primary source: SGD:S000001121
Locus tag: YHR079C
See related: AllianceGenome:SGD:S000001121; FungiDB:YHR079C; VEuPathDB:YHR079C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: ERN1
Summary: Enables RNA endonuclease activity; protein serine/threonine kinase activity; and unfolded protein binding activity. Involved in several processes, including endoplasmic reticulum unfolded protein response; fungal-type cell wall organization; and inositol metabolic process. Located in endoplasmic reticulum membrane and nucleus. Human ortholog(s) of this gene implicated in hepatocellular carcinoma. Orthologous to several human genes including ERN2 (endoplasmic reticulum to nucleus signaling 2). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See IRE1 in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (258244..261591, complement)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Role of Inositol-Requiring Enzyme 1 and Autophagy in the Pro-Fibrotic Mechanism Underlying Graves' Orbitopathy.
Title: Role of Inositol-Requiring Enzyme 1 and Autophagy in the Pro-Fibrotic Mechanism Underlying Graves' Orbitopathy.
The functional role of Ire1 in regulating autophagy and proteasomal degradation under prolonged proteotoxic stress.
Title: The functional role of Ire1 in regulating autophagy and proteasomal degradation under prolonged proteotoxic stress.
An ER-accumulated mutant of yeast Pma1 causes membrane-related stress to induce the unfolded protein response.
Title: An ER-accumulated mutant of yeast Pma1 causes membrane-related stress to induce the unfolded protein response.
Elevated constitutive expression of Hsp40 chaperone Sis1 reduces TDP-43 aggregation-induced oxidative stress in Ire1 pathway dependent-manner in yeast TDP-43 proteinopathy model of amyotrophic lateral sclerosis.
Title: Elevated constitutive expression of Hsp40 chaperone Sis1 reduces TDP-43 aggregation-induced oxidative stress in Ire1 pathway dependent-manner in yeast TDP-43 proteinopathy model of amyotrophic lateral sclerosis.
Cysteine cross-linking in native membranes establishes the transmembrane architecture of Ire1.
Title: Cysteine cross-linking in native membranes establishes the transmembrane architecture of Ire1.
Protomer alignment modulates specificity of RNA substrate recognition by Ire1.
Title: Protomer alignment modulates specificity of RNA substrate recognition by Ire1.
Vps34 and TOR Kinases Coordinate HAC1 mRNA Translation in the Presence or Absence of Ire1-Dependent Splicing.
Title: Vps34 and TOR Kinases Coordinate HAC1 mRNA Translation in the Presence or Absence of Ire1-Dependent Splicing.
Stress sensor Ire1 deploys a divergent transcriptional program in response to lipid bilayer stress.
Title: Stress sensor Ire1 deploys a divergent transcriptional program in response to lipid bilayer stress.
Unfolded protein response (UPR) is subject to inhibition by oxidative stress. Inhibition is mediated by a single evolutionarily conserved cysteine in Ire1, and affects both luminal and membrane pathways of Ire1 activation.
Title: Regulation of the unfolded protein response in yeast by oxidative stress.
Endoplasmic reticulum stress causes a diminished mRNA decay, thus resulting in the production of a population with an increased abundance of pre-HAC1 mRNA carrying an intact bipartite element, which facilitates its more efficient recruitment to Ire1p foci.
Title: Nuclear mRNA degradation tunes the gain of the unfolded protein response in Saccharomyces cerevisiae.

Submit: New GeneRIF Correction See all GeneRIFs (29)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables AMP-activated protein kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables DNA-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA endonuclease activity	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables eukaryotic translation initiation factor 2alpha kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS1 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AS121 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AT120 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2AXS139 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS14 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H2BS36 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S10 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S28 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3S57 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T3 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T45 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H4S1 kinase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	HDA more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables ribosomal protein S6 kinase activity	IEA Inferred from Electronic Annotation more info
enables unfolded protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables unfolded protein binding	IDA Inferred from Direct Assay more info	PubMed
enables unfolded protein binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in IRE1-mediated unfolded protein response	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in endoplasmic reticulum unfolded protein response	IEA Inferred from Electronic Annotation more info
involved_in endoplasmic reticulum unfolded protein response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fungal-type cell wall organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in fungal-type cell wall organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in inositol metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress	IBA Inferred from Biological aspect of Ancestor more info
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in protein localization to Golgi apparatus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to endoplasmic reticulum stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to unfolded protein	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of IRE1-TRAF2-ASK1 complex	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: bifunctional endoribonuclease/protein kinase IRE1

NP_011946.1

EC 2.7.11.1

Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; role in homeostatic adaptation to ER stress; Kar2p binds inactive Ire1p and releases from it upon ER stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

258244..261591 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179209.1 → NP_011946.1 TPA: bifunctional endoribonuclease/protein kinase IRE1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011946.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DL29, P32361

UniProtKB/TrEMBL

C7GWU1

Conserved Domains (3) summary

cd09769
Location:120 → 407

Luminal_IRE1; The Luminal domain, a dimerization domain, of the Serine/Threonine protein kinase, Inositol-requiring protein 1

cd10422
Location:984 → 1113

RNase_Ire1; RNase domain (also known as the kinase extension nuclease domain) of Ire1

cd13982
Location:672 → 981

STKc_IRE1; Catalytic domain of the Serine/Threonine kinase, Inositol-requiring protein 1