POL2 DNA polymerase epsilon catalytic subunit [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: POL2
Official Full Name: DNA polymerase epsilon catalytic subunit
Primary source: SGD:S000005206
Locus tag: YNL262W
See related: AllianceGenome:SGD:S000005206; FungiDB:YNL262W; VEuPathDB:YNL262W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DUN2
Summary: Enables SUMO binding activity; catalytic activity, acting on DNA; and single-stranded DNA binding activity. Contributes to double-stranded DNA binding activity. Involved in DNA metabolic process; mitotic DNA integrity checkpoint signaling; and mitotic sister chromatid cohesion. Located in replication fork. Part of epsilon DNA polymerase complex. Used to study cancer (multiple) and pleomorphic xanthoastrocytoma. Human ortholog(s) of this gene implicated in colorectal cancer (multiple); endometrial cancer (multiple); lung cancer (multiple); and mismatch repair cancer syndrome. Orthologous to human POLE (DNA polymerase epsilon, catalytic subunit). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See POL2 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (148212..154880)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A sensor complements the steric gate when DNA polymerase discriminates ribonucleotides.
Title: A sensor complements the steric gate when DNA polymerase ϵ discriminates ribonucleotides.
DNA polymerase epsilon leading strand signature mutations result from defects in its proofreading activity.
Title: DNA polymerase ε leading strand signature mutations result from defects in its proofreading activity.
The evolving tale of Pol2 function.
Title: The evolving tale of Pol2 function.
Enhanced polymerase activity permits efficient synthesis by cancer-associated DNA polymerase variants at low dNTP levels.
Title: Enhanced polymerase activity permits efficient synthesis by cancer-associated DNA polymerase ϵ variants at low dNTP levels.
Checkpoint-mediated DNA polymerase epsilon exonuclease activity curbing counteracts resection-driven fork collapse.
Title: Checkpoint-mediated DNA polymerase ε exonuclease activity curbing counteracts resection-driven fork collapse.
Study show that, in Saccharomyces cerevisiae, the mutation rate increases synergistically when a Polepsilon nucleotide selectivity defect is combined with a Poldelta proofreading defect, demonstrating extrinsic proofreading of Polepsilon errors by Poldelta. Combining Poldelta nucleotide selectivity and Polepsilon proofreading defects produces no synergy, indicating that Polepsilon cannot correct errors made by Poldelta.
Title: DNA polymerase δ proofreads errors made by DNA polymerase ε.
Here we show that Rad53 drives the hyper-SUMOylation of Pol2, the catalytic subunit of DNA polymerase epsilon, principally following replication forks stalling induced by nucleotide depletion
Title: The S phase checkpoint promotes the Smc5/6 complex dependent SUMOylation of Pol2, the catalytic subunit of DNA polymerase ε.
Our findings thus indicate a direct role for SUMO in Pol2-mediated DNA synthesis and a molecular basis for Smc5/6-mediated regulation of genome stability
Title: Sumoylation of the DNA polymerase ε by the Smc5/6 complex contributes to DNA replication.
DNA polymerase epsilon outcompetes double-stranded DNA for recruiting Ctf18-RFC to the replisome.
Title: Structural Basis for the Recruitment of Ctf18-RFC to the Replisome.
To dissect the Saccharomyces cerevisiae RNA polymerase II TL at individual-residue resolution, we quantitatively phenotyped nearly all TL single variants en masse.Our structural genetics approach incorporates structural and phenotypic data for high-resolution dissection of transcription mechanisms and their evolution, and is readily applicable to other essential yeast proteins.
Title: High-Resolution Phenotypic Landscape of the RNA Polymerase II Trigger Loop.

Submit: New GeneRIF Correction See all GeneRIFs (25)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 4 iron, 4 sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-directed DNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-directed DNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed DNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables DNA-directed DNA polymerase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables SUMO binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables mRNA binding	HDA more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA 3'-5' DNA exonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded DNA 3'-5' DNA exonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication proofreading	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA replication proofreading	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA-templated DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in base-excision repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in base-excision repair, gap-filling	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via nonhomologous end joining	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair via nonhomologous end joining	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in error-prone translesion synthesis	IDA Inferred from Direct Assay more info	PubMed
involved_in gene conversion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in leading strand elongation	IBA Inferred from Biological aspect of Ancestor more info
involved_in leading strand elongation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic DNA replication checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic intra-S DNA damage checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic intra-S DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic intra-S DNA damage checkpoint signaling	IPI Inferred from Physical Interaction more info	PubMed
involved_in mitotic sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleotide-excision repair, DNA gap filling	IBA Inferred from Biological aspect of Ancestor more info
involved_in nucleotide-excision repair, DNA gap filling	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of epsilon DNA polymerase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of epsilon DNA polymerase complex	IDA Inferred from Direct Assay more info	PubMed
part_of epsilon DNA polymerase complex	IEA Inferred from Electronic Annotation more info
located_in intracellular membrane-bounded organelle	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in replication fork	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DNA polymerase epsilon catalytic subunit

NP_014137.1

EC 2.7.7.7

Catalytic subunit of DNA polymerase (II) epsilon; a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; participates in leading-strand synthesis during DNA replication; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

148212..154880

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183100.1 → NP_014137.1 TPA: DNA polymerase epsilon catalytic subunit [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014137.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0T1, P21951

UniProtKB/TrEMBL

A6ZRI1

Conserved Domains (4) summary

cd05779
Location:283 → 486

DNA_polB_epsilon_exo; DEDDy 3'-5' exonuclease domain of eukaryotic DNA polymerase epsilon, a family-B DNA polymerase

cd05535
Location:545 → 1166

POLBc_epsilon; DNA polymerase type-B epsilon subfamily catalytic domain. Three DNA-dependent DNA polymerases type B (alpha, delta, and epsilon) have been identified as essential for nuclear DNA replication in eukaryotes. DNA polymerase (Pol) epsilon has been proposed ...

pfam03104
Location:121 → 442

DNA_pol_B_exo1; DNA polymerase family B, exonuclease domain

pfam08490
Location:1528 → 1920

DUF1744; Domain of unknown function (DUF1744)