U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

BUD32 serine/threonine protein kinase BUD32 [ Saccharomyces cerevisiae S288C ]

Gene ID: 853178, updated on 29-Jun-2024

Summary

Go to the top of the page Help
Gene symbol
BUD32
Gene description
serine/threonine protein kinase BUD32
Primary source
FungiDB:YGR262C
Locus tag
YGR262C
See related
SGD:S000003494; AllianceGenome:SGD:S000003494; VEuPathDB:YGR262C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
LDB14
Summary
Enables protein serine/threonine kinase activity. Involved in nucleic acid metabolic process; peptidyl-serine phosphorylation; and positive regulation of transcription by RNA polymerase II. Located in nucleus. Part of EKC/KEOPS complex. Human ortholog(s) of this gene implicated in Galloway-Mowat syndrome 4. Orthologous to human TP53RK (TP53 regulating kinase). [provided by Alliance of Genome Resources, Apr 2022]
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
See BUD32 in Genome Data Viewer
Location:
chromosome: VII
Exon count:
1
Sequence:
Chromosome: VII; NC_001139.9 (1016974..1017759, complement)

Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene Tna1p Neighboring gene Apl6p Neighboring gene steryl deacetylase Neighboring gene methionine--tRNA ligase MES1

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Acidophilic character of yeast PID261/BUD32, a putative ancestor of eukaryotic protein kinases. Facchin S, et al. Biochem Biophys Res Commun, 2002 Sep 6. PMID 12207926
  2. Structure-function analysis of yeast piD261/Bud32, an atypical protein kinase essential for normal cell life. Facchin S, et al. Biochem J, 2002 Jun 1. PMID 12023889, Free PMC Article
  3. Biochemical evidence that Saccharomyces cerevisiae YGR262c gene, required for normal growth, encodes a novel Ser/Thr-specific protein kinase. Stocchetto S, et al. FEBS Lett, 1997 Sep 1. PMID 9305753
  4. Functional homology between yeast piD261/Bud32 and human PRPK: both phosphorylate p53 and PRPK partially complements piD261/Bud32 deficiency. Facchin S, et al. FEBS Lett, 2003 Aug 14. PMID 12914926
  5. Kae1 of Saccharomyces cerevisiae KEOPS complex possesses ADP/GDP nucleotidase activity. Li QX, et al. Biochem J, 2022 Dec 9. PMID 36416748

See all (59) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. the crystal structure of Bud32/Cgi121 in complex with ADP revealed that ADP is bound in the catalytic site of Bud32 in a canonical manner
    Title: Crystal structures of the Gon7/Pcc1 and Bud32/Cgi121 complexes provide a model for the complete yeast KEOPS complex.
  2. EKC/KEOPS complex is specifically involved in the regulation of Bud9p localization downstream of Rax1p/Rax2p.
    Title: Cell polarity in Saccharomyces cerevisiae depends on proper localization of the Bud9 landmark protein by the EKC/KEOPS complex.
  3. Sch9 phosphorylates, both in vitro and in vivo, Ser258 of Bud32. This modification does not affect the catalytic properties of Bud32, but promotes its ability to associate with its substrate Grx4p and, consequently, to phosphorylate it.
    Title: Phosphorylation of the Saccharomyces cerevisiae Grx4p glutaredoxin by the Bud32p kinase unveils a novel signaling pathway involving Sch9p, a yeast member of the Akt / PKB subfamily.
Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP hydrolysis activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables ATP hydrolysis activity ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein serine kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein serine/threonine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein serine/threonine kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein serine/threonine kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein serine/threonine kinase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in cellular bud site selection HMP PubMed 
involved_in positive regulation of transcription by RNA polymerase II IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in protein phosphorylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in tRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA threonylcarbamoyladenosine metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in tRNA threonylcarbamoyladenosine metabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in telomere maintenance IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in telomere maintenance IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in telomere maintenance via recombination IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in telomere maintenance via recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of EKC/KEOPS complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of EKC/KEOPS complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in chromosome IEA
Inferred from Electronic Annotation
more info
  
located_in chromosome, telomeric region IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasm HDA PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus HDA PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

Go to the top of the page Help
Preferred Names
serine/threonine protein kinase BUD32
NP_011778.1
  • Component of KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; provides the ATPase and GTPase activities of the complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; mutation is functionally complemented by human ortholog TP53RK

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001139.9 Reference assembly

    Range
    1016974..1017759 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001181391.1 → NP_011778.1  TPA: serine/threonine protein kinase BUD32 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_011778.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VV41, P53323
    UniProtKB/TrEMBL
    A0A6A5PWD5, A6ZUS0, B3LHY0, B5VJK4, C7GNH0, C8Z9F4, G2WEY7, N1P4G6
    Conserved Domains (1) summary
    COG3642
    Location:19 → 261
    Bud32; tRNA A-37 threonylcarbamoyl transferase component Bud32 [Translation, ribosomal structure and biogenesis]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P53323.1

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Other Literature Sources
Molecular Biology Databases
Research Materials
Tools