ROT2 glucan 1,3-alpha-glucosidase ROT2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ROT2
Official Full Name: glucan 1,3-alpha-glucosidase ROT2
Primary source: SGD:S000000433
Locus tag: YBR229C
See related: AllianceGenome:SGD:S000000433; FungiDB:YBR229C; VEuPathDB:YBR229C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: GLS2
Summary: Enables Glc2Man9GlcNAc2 oligosaccharide glucosidase activity and glucan 1,3-alpha-glucosidase activity. Involved in N-glycan processing and fungal-type cell wall beta-glucan biosynthetic process. Located in endoplasmic reticulum lumen. Part of glucosidase II complex. Human ortholog(s) of this gene implicated in polycystic kidney disease; polycystic kidney disease 3; and polycystic liver disease. Orthologous to human GANAB (glucosidase II alpha subunit) and GANC (glucosidase alpha, neutral C). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See ROT2 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (676357..679221, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables Glc2Man9GlcNAc2 oligosaccharide glucosidase activity	IEA Inferred from Electronic Annotation more info
enables Glc2Man9GlcNAc2 oligosaccharide glucosidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables alpha-glucosidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables carbohydrate binding	IEA Inferred from Electronic Annotation more info
enables glucan 1,3-alpha-glucosidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables hydrolase activity, acting on glycosyl bonds	IEA Inferred from Electronic Annotation more info
enables hydrolase activity, hydrolyzing O-glycosyl compounds	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in N-glycan processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in N-glycan processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in carbohydrate metabolic process	IEA Inferred from Electronic Annotation more info
involved_in fungal-type cell wall beta-glucan biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum lumen	IDA Inferred from Direct Assay more info	PubMed
part_of glucosidase II complex	IBA Inferred from Biological aspect of Ancestor more info
part_of glucosidase II complex	IPI Inferred from Physical Interaction more info	PubMed
located_in mitochondrion	HDA more info	PubMed

General protein information

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Preferred Names: glucan 1,3-alpha-glucosidase ROT2

NP_009788.3

EC 3.2.1.207

Glucosidase II catalytic subunit; required to trim the final glucose in N-linked glycans; required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

676357..679221 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178577.3 → NP_009788.3 TPA: glucan 1,3-alpha-glucosidase ROT2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009788.3

Status: REVIEWED

UniProtKB/Swiss-Prot

P38138

UniProtKB/TrEMBL

A6ZLG7

Conserved Domains (2) summary

cd06603
Location:380 → 850

GH31_GANC_GANAB_alpha; neutral alpha-glucosidase C, neutral alpha-glucosidase AB

cd14752
Location:259 → 380

GH31_N; N-terminal domain of glycosyl hydrolase family 31 (GH31)