RAD30 DNA-directed DNA polymerase eta [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RAD30
Official Full Name: DNA-directed DNA polymerase eta
Primary source: SGD:S000002827
Locus tag: YDR419W
See related: AllianceGenome:SGD:S000002827; FungiDB:YDR419W; VEuPathDB:YDR419W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DBH1
Summary: Enables DNA-directed DNA polymerase activity. Involved in chromosome segregation; mitotic sister chromatid cohesion; and translesion synthesis. Located in mitochondrion; nucleus; and replication fork. Used to study xeroderma pigmentosum. Human ortholog(s) of this gene implicated in female breast cancer; xeroderma pigmentosum; and xeroderma pigmentosum variant type. Orthologous to human POLH (DNA polymerase eta). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See RAD30 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1303174..1305072)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Post-translational Regulation of DNA Polymerase eta, a Connection to Damage-Induced Cohesion in Saccharomyces cerevisiae.
Title: Post-translational Regulation of DNA Polymerase η, a Connection to Damage-Induced Cohesion in Saccharomyces cerevisiae.
The interaction between ubiquitin and yeast polymerase eta C terminus does not require the UBZ domain.
Title: The interaction between ubiquitin and yeast polymerase η C terminus does not require the UBZ domain.
Bypass of DNA interstrand crosslinks by a Rev1-DNA polymerase zeta complex.
Title: Bypass of DNA interstrand crosslinks by a Rev1-DNA polymerase ζ complex.
Here we show that the translesion synthesis DNA polymerase eta (Pol eta) plays a role in DNA replication under low deoxyribonucleotides condition triggered by hydroxyurea. In particular, the catalytic reaction performed by Pol eta is detrimental for RNase H deficient cells, causing DNA damage checkpoint activation and G2/M arrest
Title: RNase H activities counteract a toxic effect of Polymerase η in cells replicating with depleted dNTP pools.
Lsm12 is a novel regulator mediating Poleta deubiquitination that regulates its recruitment in response to DNA damage induced by oxidative stress.
Title: Lsm12 Mediates Deubiquitination of DNA Polymerase η To Help Saccharomyces cerevisiae Resist Oxidative Stress.
these findings provide insight into the physiological role of DNA synthesis by pol eta and have implications for our understanding of how our genome is replicated to avoid mutagenesis, genome instability and cancer
Title: DNA polymerase η contributes to genome-wide lagging strand synthesis.
Profiles of base-substitutions created by Polzeta & Poleta of Saccharomyces cerevisiae were analyzed. Poleta created deletions at the lesion in more than 50% of the TLS products, but the formation of the deletions was suppressed by the presence of Polzeta. Polzeta & Poleta cooperatively facilitated the TLS reaction over an abasic site in vitro, suggesting that these two polymerases can cooperate in efficient and high f...
Title: NGS-based analysis of base-substitution signatures created by yeast DNA polymerase eta and zeta on undamaged and abasic DNA templates in vitro.
This study provides further understanding of the mutation mechanism of alkylated lesion for yeast DNA polymerase eta.
Title: Kinetic analysis of bypass of O(6)- methylguanine by the catalytic core of yeast DNA polymerase eta.
These data indicate that under certain conditions, pol eta can compete with the cell's replicases and gain access to undamaged genomic DNA.
Title: Unlocking the steric gate of DNA polymerase η leads to increased genomic instability in Saccharomyces cerevisiae.
The stability of Pol eta, but not its mRNA level, is cell cycle stage dependent. The half-life of the polymerase is more than fourfold shorter in G1-arrested cells than in those at G2/M.
Title: The steady-state level and stability of TLS polymerase eta are cell cycle dependent in the yeast S. cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-directed DNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-directed DNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed DNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables damaged DNA binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in error-free translesion synthesis	IDA Inferred from Direct Assay more info	PubMed
involved_in error-free translesion synthesis	IEA Inferred from Electronic Annotation more info
involved_in error-prone translesion synthesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in error-prone translesion synthesis	IDA Inferred from Direct Assay more info	PubMed
involved_in error-prone translesion synthesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to radiation	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in intracellular membrane-bounded organelle	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
is_active_in replication fork	IBA Inferred from Biological aspect of Ancestor more info
located_in replication fork	IPI Inferred from Physical Interaction more info	PubMed
is_active_in site of double-strand break	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: DNA-directed DNA polymerase eta

NP_010707.3

EC 2.7.7.7

DNA polymerase eta (pol eta); involved in translesion synthesis during post-replication repair; catalyzes synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; involved in formation of post-replicative damage-induced genome-wide cohesion; may also have role in protection against mitochondrial mutagenesis; may possibly be involved in meiosis; mutations in human pol eta are responsible for XPV

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1303174..1305072

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180727.3 → NP_010707.3 TPA: DNA-directed DNA polymerase eta [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010707.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VT49, Q04049

UniProtKB/TrEMBL

A6ZZ16, B3LFN7, B5VGU9, C8Z5Y1, N1P5J8

Conserved Domains (1) summary

cd01702
Location:27 → 505

PolY_Pol_eta; DNA Polymerase eta