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STE5 Ste5p [ Saccharomyces cerevisiae S288C ]

Gene ID: 851680, updated on 18-Sep-2024

Summary

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Official Symbol
STE5
Official Full Name
Ste5p
Primary source
SGD:S000002510
Locus tag
YDR103W
See related
AllianceGenome:SGD:S000002510; FungiDB:YDR103W; VEuPathDB:YDR103W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
HMD3; NUL3
Summary
Enables G-protein beta-subunit binding activity; MAP-kinase scaffold activity; and phosphatidylinositol-4,5-bisphosphate binding activity. Involved in several processes, including invasive growth in response to glucose limitation; negative regulation of MAPK cascade; and pheromone-dependent signal transduction involved in conjugation with cellular fusion. Located in cytoplasm; mating projection tip membrane; and nucleus. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See STE5 in Genome Data Viewer
Location:
chromosome: IV
Exon count:
1
Sequence:
Chromosome: IV; NC_001136.10 (658350..661103)

Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene putative hydrolase Neighboring gene uncharacterized protein Neighboring gene Spo71p Neighboring gene Tms1p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Evolution of synthetic signaling scaffolds by recombination of modular protein domains. Lai A, et al. ACS Synth Biol, 2015 Jun 19. PMID 25587847
  2. Structural determinants of the specificity of a membrane binding domain of the scaffold protein Ste5 of budding yeast: implications in signaling by the scaffold protein in MAPK pathway. Bhunia A, et al. Biochim Biophys Acta, 2012 May. PMID 22285780
  3. Quantitative effect of scaffold abundance on signal propagation. Chapman SA, et al. Mol Syst Biol, 2009. PMID 19888208, Free PMC Article
  4. A mechanism for cell-cycle regulation of MAP kinase signaling in a yeast differentiation pathway. Strickfaden SC, et al. Cell, 2007 Feb 9. PMID 17289571, Free PMC Article
  5. Function of the MAPK scaffold protein, Ste5, requires a cryptic PH domain. Garrenton LS, et al. Genes Dev, 2006 Jul 15. PMID 16847350, Free PMC Article

See all (148) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Effects of HSP70 chaperones Ssa1 and Ssa2 on Ste5 scaffold and the mating mitogen-activated protein kinase (MAPK) pathway in Saccharomyces cerevisiae.
    Title: Effects of HSP70 chaperones Ssa1 and Ssa2 on Ste5 scaffold and the mating mitogen-activated protein kinase (MAPK) pathway in Saccharomyces cerevisiae.
  2. Pkc1 triggers dispersal of Ste5 from mating projections upon mechanically induced stress and during cell-cell fusion, leading to inhibition of the MAPK Fus3.
    Title: Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5.
  3. phosphorylation of the yeast Ggamma subunit (Ste18), together with Fus3 docking on Ste5, controls the rate and stability of Ste5/plasma membrane association.
    Title: Negative Feedback Phosphorylation of Gγ Subunit Ste18 and the Ste5 Scaffold Synergistically Regulates MAPK Activation in Yeast.
  4. CDK and MAPK modify shared sites, which are most extensively phosphorylated when both kinases are active and able to bind their docking sites on Ste5. synergistically regulating signaling dynamics.
    Title: CDK and MAPK Synergistically Regulate Signaling Dynamics via a Shared Multi-site Phosphorylation Region on the Scaffold Protein Ste5.
  5. defining regions of Ste5 that mediate nuclear export
    Title: Mapping regions in Ste5 that support Msn5-dependent and -independent nuclear export.
  6. The Ras-binding-domain-like region in the Ste11 protein that is required specifically for the kinase to function with Ste5 in the mating pathway
    Title: Structurally unique interaction of RBD-like and PH domains is crucial for yeast pheromone signaling.
  7. Structural determinants of the specificity of a membrane binding domain of the scaffold protein Ste5 of budding yeast: implications in signaling by the scaffold protein in MAPK pathway.
    Title: Structural determinants of the specificity of a membrane binding domain of the scaffold protein Ste5 of budding yeast: implications in signaling by the scaffold protein in MAPK pathway.
  8. findings show that the scaffold protein Ste5, which organizes the yeast mating mitogen-activated protein kinase pathway, does not use sequestration to prevent misactivation of the mating response; Ste5 appears to use a conformation mechanism
    Title: Conformational control of the Ste5 scaffold protein insulates against MAP kinase misactivation.
  9. The signaling proteins Ste5 and Ste20 have recognition sequences, or "docking" sites, for G1/S (interphase) cyclins. These docking sites bind preferentially to the cyclin Cln2, and strongly enhance Cln2-driven in vivo substrate phosphorylation.
    Title: Cyclin-specific docking motifs promote phosphorylation of yeast signaling proteins by G1/S Cdk complexes.
  10. a switch-like dissociation of Fus3 from Ste5 that is necessary to generate the switch-like mating response
    Title: The scaffold protein Ste5 directly controls a switch-like mating decision in yeast.
Submit: New GeneRIF Correction See all GeneRIFs (19)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables G-protein beta-subunit binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables MAP-kinase scaffold activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables MAP-kinase scaffold activity IPI
Inferred from Physical Interaction
more info
 PubMed 
enables kinase binding IEA
Inferred from Electronic Annotation
more info
  
enables phosphatidylinositol-4,5-bisphosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in MAPK cascade IEA
Inferred from Electronic Annotation
more info
  
involved_in invasive growth in response to glucose limitation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of MAPK cascade IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of protein phosphorylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in response to pheromone IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in mating projection tip IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mating projection tip membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IMP
Inferred from Mutant Phenotype
more info
 PubMed 

General protein information

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Preferred Names
Ste5p
NP_010388.1
  • Pheromone-responsive MAPK scaffold protein; couples activation of the G-protein-coupled pheromone receptor to MAPK activation; intramolecular interaction of PH and VWA domains blocks activation of assembled signaling cascade components (Ste11p, Ste7p and Fus3p) under basal conditions; Gbeta-gamma (Ste4p-Ste18p)-dependent docking at the plasma membrane and binding of PI(4,5)P2 by the PH domain relieves autoinhibition, resulting in pheromone-dependent pathway activation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001136.10 Reference assembly

    Range
    658350..661103
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001180411.1 → NP_010388.1  TPA: Ste5p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_010388.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VS89, P32917
    UniProtKB/TrEMBL
    B3LGH2, B5VG01, C8Z519
    Conserved Domains (3) summary
    pfam11610
    Location:287 → 316
    Ste5; Scaffold protein Ste5, Fus3-binding region
    pfam12194
    Location:594 → 775
    Ste5_C; Protein kinase Fus3-binding
    cl17238
    Location:176 → 207
    RING_Ubox; The superfamily of RING finger (Really Interesting New Gene) domain and U-box domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P32917.2

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