RAD59 Rad59p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RAD59
Official Full Name: Rad59p
Primary source: SGD:S000002217
Locus tag: YDL059C
See related: AllianceGenome:SGD:S000002217; FungiDB:YDL059C; VEuPathDB:YDL059C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Involved in DNA metabolic process and regulation of DNA biosynthetic process. Located in mitochondrial matrix and nucleus. Orthologous to human RAD52 (RAD52 homolog, DNA repair protein). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See RAD59 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (344237..344953, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

propose that Rad59 promotes fork progression when Okazaki fragment processing is compromised and counteracts PCNA-K107 mediated cell cycle arrest
Title: Unligated Okazaki Fragments Induce PCNA Ubiquitination and a Requirement for Rad59-Dependent Replication Fork Progression.
SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination
Title: SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination.
We show that Rad59-facilitated Y' translocations on X-only telomeres delay the onset of senescence while preparing ground for type I survivor formation.
Title: Rad59-facilitated acquisition of Y' elements by short telomeres delays the onset of senescence.
Findings suggest a Rad52/Rad59-dependent, recombination pathway that could ultimately lead to the removal of faulty flaps in conjunction with cohesion establishment factors.
Title: Rad52/Rad59-dependent recombination as a means to rectify faulty Okazaki fragment processing.
These results suggest that Rad59 influences the rescue of replication lesions by multiple recombination factors.
Title: Alleles of the homologous recombination gene, RAD59, identify multiple responses to disrupted DNA replication in Saccharomyces cerevisiae.
Data show that RSC physically interacts with the recombination protein Rad59 and functions in homologous recombination.
Title: RSC facilitates Rad59-dependent homologous recombination between sister chromatids by promoting cohesin loading at DNA double-strand breaks.
at least one role of Rad59 in translocation formation by single-strand annealing is supporting the machinery required for cleavage of non-homologous tails
Title: RAD59 and RAD1 cooperate in translocation formation by single-strand annealing in Saccharomyces cerevisiae.
RAD59 was found to play a particularly significant role in recombination between the short substrates that was partially independent of that of Rad52 DNA Repair and Recombination Protein
Title: RAD59 is required for efficient repair of simultaneous double-strand breaks resulting in translocations in Saccharomyces cerevisiae.
examined the ssDNA annealing activity of Rad52 and Rad59 proteins and found significant differences in their biochemical properties.
Title: DNA annealing mediated by Rad52 and Rad59 proteins.
S. cerevisiae Rad59 protein and its eukaryotic counterparts function by binding to single-stranded DNA formed as intermediates of recombination rather than by binding to the unprocessed DNA double-strand break.
Title: The DNA binding preference of RAD52 and RAD59 proteins: implications for RAD52 and RAD59 protein function in homologous recombination.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA amplification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via homologous recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via single-strand annealing	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via single-strand annealing	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via single-strand annealing	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitochondrial DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of DNA biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomere maintenance via recombination	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in mitochondrial matrix	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IGI Inferred from Genetic Interaction more info	PubMed

General protein information

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Preferred Names: Rad59p

NP_010224.1

Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; regulates replication fork progression in DNA ligase I-deficient cells; paralog of Rad52p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

344237..344953 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180118.1 → NP_010224.1 TPA: Rad59p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010224.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VRT7, Q12223

UniProtKB/TrEMBL

A6ZXR6, B3LGV7, C8Z4L0, N1P481

Conserved Domains (1) summary

cl01936
Location:22 → 238

Rad52_Rad22; Rad52/22 family double-strand break repair protein