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TPST2 tyrosylprotein sulfotransferase 2 [ Homo sapiens (human) ]

Gene ID: 8459, updated on 5-Mar-2024

Summary

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Official Symbol
TPST2provided by HGNC
Official Full Name
tyrosylprotein sulfotransferase 2provided by HGNC
Primary source
HGNC:HGNC:12021
See related
Ensembl:ENSG00000128294 MIM:603126; AllianceGenome:HGNC:12021
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
TPST-2; TANGO13B
Summary
The protein encoded by this gene catalyzes the O-sulfation of tyrosine residues within acidic regions of proteins. The encoded protein is a type II integral membrane protein found in the Golgi body. Alternative splicing produces multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018]
Expression
Ubiquitous expression in pancreas (RPKM 24.6), placenta (RPKM 15.2) and 24 other tissues See more
Orthologs
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Genomic context

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See TPST2 in Genome Data Viewer
Location:
22q12.1
Exon count:
10
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 22 NC_000022.11 (26521996..26590132, complement)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 22 NC_060946.1 (26984404..27052464, complement)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 22 NC_000022.10 (26917962..26986096, complement)

Chromosome 22 - NC_000022.11Genomic Context describing neighboring genes Neighboring gene MED14-independent group 3 enhancer GRCh37_chr22:26889254-26890453 Neighboring gene SRR1 domain containing Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 13570 Neighboring gene tuftelin interacting protein 11 Neighboring gene BRD4-independent group 4 enhancer GRCh37_chr22:26902046-26903245 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18792 Neighboring gene H3K27ac hESC enhancer GRCh37_chr22:26908724-26909261 Neighboring gene TFIP11 divergent transcript Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:26937311-26937812 Neighboring gene Sharpr-MPRA regulatory region 3066 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:26951753-26952722 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:26959537-26960060 Neighboring gene microRNA 548j Neighboring gene hESC enhancers GRCh37_chr22:26963727-26964250 and GRCh37_chr22:26964251-26964774 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:26964775-26965298 Neighboring gene high mobility group box 1 pseudogene 10 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18793 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancers GRCh37_chr22:26975739-26976656 and GRCh37_chr22:26976657-26977573 Neighboring gene H3K27ac hESC enhancer GRCh37_chr22:26985294-26985794 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 13573 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18794 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18795 Neighboring gene crystallin beta A4 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr22:27003362-27004004 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 18796 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 13574 Neighboring gene crystallin beta B1 Neighboring gene OCT4-NANOG-H3K27ac hESC enhancer GRCh37_chr22:27009656-27010334 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:27013045-27013722 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:27014401-27015076 Neighboring gene CRISPRi-validated cis-regulatory element chr22.1058 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:27037805-27038669 Neighboring gene Sharpr-MPRA regulatory region 8650 Neighboring gene Sharpr-MPRA regulatory region 12592 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr22:27041123-27041688 Neighboring gene iron-sulfur cluster assembly 2 pseudogene 1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Computational Evidence for the Catalytic Mechanism of Tyrosylprotein Sulfotransferases: A Density Functional Theory Investigation. Marforio TD, et al. Biochemistry, 2015 Jul 21. PMID 26108987
  2. Sequence analysis of the human tyrosylprotein sulfotransferase-2 gene in subjects with chronic pancreatitis. Rosendahl J, et al. Pancreatology, 2010. PMID 20460947, Free PMC Article
  3. Sulfation of a FLAG tag mediated by SLC35B2 and TPST2 affects antibody recognition. Guo XY, et al. PLoS One, 2021. PMID 33951064, Free PMC Article
  4. Genetic variations in the host dependency factors ALCAM and TPST2 impact HIV-1 disease progression. Kruize Z, et al. AIDS, 2020 Jul 15. PMID 32287057
  5. Crystal structure of human tyrosylprotein sulfotransferase-2 reveals the mechanism of protein tyrosine sulfation reaction. Teramoto T, et al. Nat Commun, 2013. PMID 23481380, Free PMC Article

See all (33) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Sulfation of a FLAG tag mediated by SLC35B2 and TPST2 affects antibody recognition.
    Title: Sulfation of a FLAG tag mediated by SLC35B2 and TPST2 affects antibody recognition.
  2. Genetic variations in the host dependency factors ALCAM and TPST2 impact HIV-1 disease progression.
    Title: Genetic variations in the host dependency factors ALCAM and TPST2 impact HIV-1 disease progression.
  3. Using a quantum mechanics protocol of alanine scanning, the study identified unequivocally the role of the amino acids involved in the TPST-2 catalytic mechanism.
    Title: Computational Evidence for the Catalytic Mechanism of Tyrosylprotein Sulfotransferases: A Density Functional Theory Investigation.
  4. the first crystal structure of the human tyrosylprotein sulfotransferase isoform 2 complexed with a substrate peptide (C4P5Y3) derived from complement C4 and 3'-phosphoadenosine-5'-phosphate at 1.9 A resolution, is reported.
    Title: Crystal structure of human tyrosylprotein sulfotransferase-2 reveals the mechanism of protein tyrosine sulfation reaction.
  5. The p.R153H variant was found in a family with hereditary pancreatitis; however, it did not segregate with the disease.
    Title: Sequence analysis of the human tyrosylprotein sulfotransferase-2 gene in subjects with chronic pancreatitis.
  6. Observational study of gene-disease association. (HuGE Navigator)
    Title: Sequence analysis of the human tyrosylprotein sulfotransferase-2 gene in subjects with chronic pancreatitis.
  7. The kinetic parameters of tyrosylprotein sulfotransferase-1 and -2, catalyzing tyrosine sulfation of CCR8 peptides, were determined using liquid chromatography electrospray ionisation mass spectrometry.
    Title: Mass spectrometric kinetic analysis of human tyrosylprotein sulfotransferase-1 and -2.
  8. Tyrosine sulfation of CCR5 N-terminal peptide follows a discrete pattern and temporal sequence
    Title: Tyrosine sulfation of CCR5 N-terminal peptide by tyrosylprotein sulfotransferases 1 and 2 follows a discrete pattern and temporal sequence.
  9. Shear stress-dependent upregulation of TPST2 in human endothelium is mediated by a tyrosine kinase-dependent pathway
    Title: Shear stress mediates tyrosylprotein sulfotransferase isoform shift in human endothelial cells.
Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 gp120 is sulfated at positions Y173 and Y177 in the V2 domain by tyrosyl sulfotransferase 2 (TPST2), leading to modulate gp120 epitope exposure and neutralization sensitivity PubMed
env Sulfation of CCR5 by tyrosylprotein sulfotranferase-2 is crucial for mediating interaction with HIV-1 gp120. The pattern and the rate of sulfation for CCR5 depend on the number of amino acids N-terminal of Tyr-3 PubMed
env The chemokine receptor CCR5 is posttranslationally modified by sulfation of its N-terminal tyrosines; sulfated tyrosines contribute to the binding of CCR5 to MIP-1 alpha, MIP-1 beta, and HIV-1 gp120/CD4 complexes and to the ability of HIV-1 to enter cells PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables protein homodimerization activity IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein-tyrosine sulfotransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein-tyrosine sulfotransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein-tyrosine sulfotransferase activity TAS
Traceable Author Statement
more info
  
Process Evidence Code Pubs
involved_in 3'-phosphoadenosine 5'-phosphosulfate metabolic process TAS
Traceable Author Statement
more info
  
involved_in peptidyl-tyrosine sulfation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in peptidyl-tyrosine sulfation IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
is_active_in Golgi apparatus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in Golgi membrane TAS
Traceable Author Statement
more info
  
located_in endoplasmic reticulum IDA
Inferred from Direct Assay
more info
  

General protein information

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Preferred Names
protein-tyrosine sulfotransferase 2
Names
transport and golgi organization 13 homolog B
tyrosylprotein phosphotransferase 2
NP_001008566.1
NP_001349851.1
NP_001349852.1
NP_003586.3
XP_024308062.1
XP_047297505.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001008566.3 → NP_001008566.1  protein-tyrosine sulfotransferase 2 isoform 1

    See identical proteins and their annotated locations for NP_001008566.1

    Status: REVIEWED

    Source sequence(s)
    Z95115
    Consensus CDS
    CCDS13839.1
    UniProtKB/Swiss-Prot
    B3KQA7, O60704, Q6FI98, Q9H0V4
    UniProtKB/TrEMBL
    B7Z976
    Related
    ENSP00000381180.2, ENST00000398110.6
    Conserved Domains (1) summary
    pfam13469
    Location:71 → 262
    Sulfotransfer_3; Sulfotransferase family

  2. NM_001362922.2 → NP_001349851.1  protein-tyrosine sulfotransferase 2 isoform 1

    Status: REVIEWED

    Source sequence(s)
    Z95115
    Consensus CDS
    CCDS13839.1
    UniProtKB/Swiss-Prot
    B3KQA7, O60704, Q6FI98, Q9H0V4
    UniProtKB/TrEMBL
    B7Z976
    Conserved Domains (1) summary
    pfam13469
    Location:71 → 262
    Sulfotransfer_3; Sulfotransferase family

  3. NM_001362923.2 → NP_001349852.1  protein-tyrosine sulfotransferase 2 isoform 2

    Status: REVIEWED

    Source sequence(s)
    Z95115
    Conserved Domains (1) summary
    pfam13469
    Location:125 → 316
    Sulfotransfer_3; Sulfotransferase family

  4. NM_003595.5 → NP_003586.3  protein-tyrosine sulfotransferase 2 isoform 1

    See identical proteins and their annotated locations for NP_003586.3

    Status: REVIEWED

    Source sequence(s)
    Z95115
    Consensus CDS
    CCDS13839.1
    UniProtKB/Swiss-Prot
    B3KQA7, O60704, Q6FI98, Q9H0V4
    UniProtKB/TrEMBL
    B7Z976
    Related
    ENSP00000339813.4, ENST00000338754.9
    Conserved Domains (1) summary
    pfam13469
    Location:71 → 262
    Sulfotransfer_3; Sulfotransferase family

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000022.11 Reference GRCh38.p14 Primary Assembly

    Range
    26521996..26590132 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_047441549.1 → XP_047297505.1  protein-tyrosine sulfotransferase 2 isoform X2

  2. XM_024452294.2 → XP_024308062.1  protein-tyrosine sulfotransferase 2 isoform X1

    Conserved Domains (1) summary
    pfam13469
    Location:141 → 332
    Sulfotransfer_3; Sulfotransferase family

RNA

  1. XR_007067981.1 RNA Sequence

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060946.1 Alternate T2T-CHM13v2.0

    Range
    26984404..27052464 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O60704.1 GenPept UniProtKB/Swiss-Prot:O60704

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