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TOC33 translocon at the outer envelope membrane of chloroplasts 33 [ Arabidopsis thaliana (thale cress) ]

Gene ID: 839248, updated on 18-Sep-2024

Summary

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Official Symbol
TOC33
Official Full Name
translocon at the outer envelope membrane of chloroplasts 33
Primary source
TAIR:AT1G02280
Locus tag
AT1G02280
See related
Araport:AT1G02280
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Arabidopsis thaliana (ecotype: Columbia)
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as
ATTOC33; PLASTID PROTEIN IMPORT 1; PPI1; T6A9.32; translocon at the outer envelope membrane of chloroplasts 33
Summary
Encodes a GTP-binding GTP-ase. Component of the chloroplast protein import machinery. Required for import of POR B into plastids. Toc33 phosphorylation may not play an important role in vivo.
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Genomic context

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See TOC33 in Genome Data Viewer
Location:
chromosome: 1
Exon count:
8
Sequence:
Chromosome: 1; NC_003070.9 (448423..450426, complement)

Chromosome 1 - NC_003070.9Genomic Context describing neighboring genes Neighboring gene Calcium-binding endonuclease/exonuclease/phosphatase family Neighboring gene miscRNA Neighboring gene uncharacterized protein Neighboring gene Cysteine proteinases superfamily protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Unusual nucleotide-binding properties of the chloroplast protein import receptor, atToc33. Aronsson H, et al. FEBS Lett, 2003 Jun 5. PMID 12782294
  2. Dimerization of Toc-GTPases at the chloroplast protein import machinery. Weibel P, et al. J Biol Chem, 2003 Sep 26. PMID 12869544
  3. Substrate binding disrupts dimerization and induces nucleotide exchange of the chloroplast GTPase Toc33. Oreb M, et al. Biochem J, 2011 Jun 1. PMID 21434866, Free PMC Article
  4. Nucleotide binding and dimerization at the chloroplast pre-protein import receptor, atToc33, are not essential in vivo but do increase import efficiency. Aronsson H, et al. Plant J, 2010 Jul. PMID 20444229
  5. In vivo assessment of the significance of phosphorylation of the Arabidopsis chloroplast protein import receptor, atToc33. Aronsson H, et al. FEBS Lett, 2006 Jan 23. PMID 16412428

See all (34) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structural and Functional Heat Stress Responses of Chloroplasts of Arabidopsis thaliana.
    Title: Structural and Functional Heat Stress Responses of Chloroplasts of Arabidopsis thaliana.
  2. Toc64 participates in chloroplast protein import and cooperates in part with Toc33.
    Title: Toc33 and Toc64-III cooperate in precursor protein import into the chloroplasts of Arabidopsis thaliana.
  3. [review] Homodimerization of Toc33 prevents nucleotide exchange, thereby locking the receptor in the guanosine diphosphate (GDP)-loaded state and preventing further activity.
    Title: Dimerization of TOC receptor GTPases and its implementation for the control of protein import into chloroplasts.
  4. Pre-protein binding induces a dissociation of the Toc33 homodimer and results in exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP).
    Title: Substrate binding disrupts dimerization and induces nucleotide exchange of the chloroplast GTPase Toc33.
  5. Neither nucleotide binding nor dimerization at atToc33 is essential for chloroplast import.
    Title: Nucleotide binding and dimerization at the chloroplast pre-protein import receptor, atToc33, are not essential in vivo but do increase import efficiency.
  6. Toc33-R130A mutation reduces homodimerization and affects the interaction of Toc33 with Toc159 during protein import into chloroplasts.
    Title: Toc receptor dimerization participates in the initiation of membrane translocation during protein import into chloroplasts.
  7. Toc33 and Toc159 form heterodimer in vivo.
    Title: In vivo interaction between atToc33 and atToc159 GTP-binding domains demonstrated in a plant split-ubiquitin system.
  8. Toc33 GTPase homodimerization and protein translocation into chloroplasts are pH sensitive.
    Title: pH sensitivity of the GTPase Toc33 as a regulatory circuit for protein translocation into chloroplasts.
  9. analysis of Arabidopsis Toc33, Toc34, and Toc159-GTPase homodimers
    Title: On the significance of Toc-GTPase homodimers.
  10. Dimerization is independent of nucleotide loading state, based on crystal structures of dimeric Pisum sativum Toc34 and monomeric Arabidopsis thaliana Toc33.
    Title: The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures.
Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

NM_001123743.2
NM_100108.5

Gene Ontology Provided by TAIR

Function Evidence Code Pubs
enables GTP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables GTPase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein-transporting ATPase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in intracellular protein transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within protein targeting to chloroplast TAS
Traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
located_in chloroplast HDA PubMed 
located_in chloroplast ISM
Inferred from Sequence Model
more info
  
located_in chloroplast envelope HDA PubMed 
located_in chloroplast outer membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in extracellular region HDA PubMed 
located_in intracellular anatomical structure TAS
Traceable Author Statement
more info
 PubMed 

General protein information

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Preferred Names
translocon at the outer envelope membrane of chloroplasts 33
NP_001117215.1
  • translocon at the outer envelope membrane of chloroplasts 33 (TOC33); FUNCTIONS IN: protein binding, GTP binding, GTPase activity, protein homodimerization activity; INVOLVED IN: protein targeting to chloroplast; LOCATED IN: chloroplast outer membrane, chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: AIG1 (InterPro:IPR006703), Chloroplast protein import component Toc34 (InterPro:IPR005688); BEST Arabidopsis thaliana protein match is: translocon at the outer envelope membrane of chloroplasts 34 (TAIR:AT5G05000.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
NP_171730.1
  • translocon at the outer envelope membrane of chloroplasts 33 (TOC33); FUNCTIONS IN: protein binding, GTP binding, GTPase activity, protein homodimerization activity; INVOLVED IN: protein targeting to chloroplast; LOCATED IN: chloroplast outer membrane, chloroplast, chloroplast envelope; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: AIG1 (InterPro:IPR006703), Chloroplast protein import component Toc34 (InterPro:IPR005688); BEST Arabidopsis thaliana protein match is: translocon at the outer envelope membrane of chloroplasts 34 (TAIR:AT5G05000.1); Has 1041 Blast hits to 1035 proteins in 244 species: Archae - 0; Bacteria - 399; Metazoa - 251; Fungi - 18; Plants - 325; Viruses - 0; Other Eukaryotes - 48 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003070.9 Reference assembly

    Range
    448423..450426 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_100108.5 → NP_171730.1  translocon at the outer envelope membrane of chloroplasts 33 [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_171730.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    O23680, Q94B42, Q9GDD3
    UniProtKB/TrEMBL
    A0A178W9C6, A0A5S9S5U9
    Conserved Domains (1) summary
    cl38936
    Location:1 → 296
    P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases

  2. NM_001123743.2 → NP_001117215.1  translocon at the outer envelope membrane of chloroplasts 33 [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_001117215.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    O23680, Q94B42, Q9GDD3
    UniProtKB/TrEMBL
    A0A178W9C6, A0A5S9S5U9
    Conserved Domains (1) summary
    cl38936
    Location:1 → 296
    P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O23680.1

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