APX1 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: APX1
Primary source: TAIR:AT1G07890
Locus tag: AT1G07890
See related: Araport:AT1G07890
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ASCORBATE PEROXIDASE; ascorbate peroxidase 1; ATAPX01; ATAPX1; CS1; F24B9.2; F24B9_2; maternal effect embryo arrest 6; MEE6
Summary: Encodes a cytosolic ascorbate peroxidase APX1. Ascorbate peroxidases are enzymes that scavenge hydrogen peroxide in plant cells. Eight types of APX have been described for Arabidopsis: three cytosolic (APX1, APX2, APX6), two chloroplastic types (stromal sAPX, thylakoid tAPX), and three microsomal (APX3, APX4, APX5) isoforms. At least part of the induction of heat shock proteins during light stress in Arabidopsis is mediated by H2O2 that is scavenged by APX1. Expression of the gene is downregulated in the presence of paraquat, an inducer of photoxidative stress.
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Genomic context

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Location:: chromosome: 1

Exon count:: 9

Sequence:: Chromosome: 1; NC_003070.9 (2437281..2439665)

Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Loss-of-function of APX1 results in tolerance to Se stress.
Title: Loss-of-function mutations in the APX1 gene result in enhanced selenium tolerance in Arabidopsis thaliana.
our results suggest that APX1 gene knockout results in enhanced Pb tolerance mainly through activating the expression of the ATP-bind cassette (ABC)-type transporters and at least partially through GSH -dependent PC synthesis pathway by coordinated control of gene expression.
Title: A role for APX1 gene in lead tolerance in Arabidopsis thaliana.
S-nitrosylation of APX1 at cysteine (Cys)-32 enhances its enzymatic activity of scavenging hydrogen peroxide, leading to the increased resistance to oxidative stress.
Title: S-nitrosylation positively regulates ascorbate peroxidase activity during plant stress responses.
Studies indicate that in apx1/cat2 double-mutant plants, a DNA damage response is activated, suppressing growth via a WEE1 kinase-dependent cell-cycle checkpoint.
Title: Extranuclear protection of chromosomal DNA from oxidative stress.
cytosolic APX1 plays a key role in the acclimation of plants to a combination of drought and heat stress
Title: Ascorbate peroxidase 1 plays a key role in the response of Arabidopsis thaliana to stress combination.
Ascorbate peroxidase activity was higher in ascorbate-deficient mutants.
Title: Antioxidant status, peroxidase activity, and PR protein transcript levels in ascorbate-deficient Arabidopsis thaliana vtc mutants.
Data suggest that ascorbate peroxidase is particularly important for photoprotection during the early greening process in Arabidopsis.
Title: Diverse roles for chloroplast stromal and thylakoid-bound ascorbate peroxidases in plant stress responses.
Lack of a cytosolic hydrogen peroxide removal enzyme triggers a signal which results in stunted growth and enhanced sensitivity to oxidative stress.
Title: Double mutants deficient in cytosolic and thylakoid ascorbate peroxidase reveal a complex mode of interaction between reactive oxygen species, plant development, and response to abiotic stresses.
Our study points to a key role for the cytosol in protecting the chloroplast during light stress and provides evidence for cross-compartment protection of thylakoid and stromal/mitochondrial APXs by cytosolic APX1.
Title: Cytosolic ascorbate peroxidase 1 is a central component of the reactive oxygen gene network of Arabidopsis.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_001035914.2

Supported by ESTs:

NM_001084012.1

Supported by ESTs:

NM_001084013.1

Supported by ESTs:

NM_001123772.2

Supported by ESTs:

NM_001331739.1

Supported by ESTs:

NM_100663.4

Supported by ESTs:

NM_179276.2

Supported by ESTs:

NM_202057.2

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables L-ascorbate peroxidase activity	IEA Inferred from Electronic Annotation more info
enables L-ascorbate peroxidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables L-ascorbate peroxidase activity	TAS Traceable Author Statement more info	PubMed
enables heme binding	IEA Inferred from Electronic Annotation more info
enables p-coumarate 3-hydroxylase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to oxidative stress	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within embryo development ending in seed dormancy	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within hydrogen peroxide catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lignin biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within lignin biosynthetic process	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within lignin biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within lignin biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within phenylpropanoid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to heat	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within response to reactive oxygen species	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi apparatus	HDA more info	PubMed
located_in chloroplast	HDA more info	PubMed
located_in chloroplast stroma	HDA more info	PubMed
located_in cytoplasm	ISM Inferred from Sequence Model more info
located_in cytoplasmic stress granule	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	HDA more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info	PubMed
located_in extracellular region	HDA more info	PubMed
located_in plant-type cell wall	HDA more info	PubMed
located_in plasma membrane	HDA more info	PubMed
located_in plasmodesma	HDA more info	PubMed

General protein information

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Preferred Names: ascorbate peroxidase 1

NP_001030991.2

ascorbate peroxidase 1 (APX1); FUNCTIONS IN: L-ascorbate peroxidase activity; INVOLVED IN: in 6 processes; LOCATED IN: cytosol, cell wall, chloroplast, plasma membrane; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Haem peroxidase (InterPro:IPR010255), Plant ascorbate peroxidase (InterPro:IPR002207), Peroxidases heam-ligand binding site (InterPro:IPR019793), Peroxidase, active site (InterPro:IPR019794), Haem peroxidase, plant/fungal/bacterial (InterPro:IPR002016); BEST Arabidopsis thaliana protein match is: ascorbate peroxidase 2 (TAIR:AT3G09640.2); Has 8811 Blast hits to 8057 proteins in 1265 species: Archae - 75; Bacteria - 2912; Metazoa - 21; Fungi - 794; Plants - 3291; Viruses - 0; Other Eukaryotes - 1718 (source: NCBI BLink).

NP_001077481.1

ascorbate peroxidase 1 (APX1); FUNCTIONS IN: L-ascorbate peroxidase activity; INVOLVED IN: response to reactive oxygen species, response to salt stress, response to heat, hydrogen peroxide catabolic process, embryo development ending in seed dormancy; LOCATED IN: cytosol, chloroplast, plasma membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Haem peroxidase (InterPro:IPR010255), Plant ascorbate peroxidase (InterPro:IPR002207), Peroxidases heam-ligand binding site (InterPro:IPR019793), Peroxidase, active site (InterPro:IPR019794), Haem peroxidase, plant/fungal/bacterial (InterPro:IPR002016); BEST Arabidopsis thaliana protein match is: ascorbate peroxidase 2 (TAIR:AT3G09640.2); Has 8882 Blast hits to 8124 proteins in 1284 species: Archae - 75; Bacteria - 2940; Metazoa - 21; Fungi - 800; Plants - 3322; Viruses - 0; Other Eukaryotes - 1724 (source: NCBI BLink).

NP_001077482.1

ascorbate peroxidase 1 (APX1); FUNCTIONS IN: L-ascorbate peroxidase activity; INVOLVED IN: response to reactive oxygen species, response to salt stress, response to heat, hydrogen peroxide catabolic process, embryo development ending in seed dormancy; LOCATED IN: cytosol, chloroplast, plasma membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Haem peroxidase (InterPro:IPR010255), Plant ascorbate peroxidase (InterPro:IPR002207), Peroxidases heam-ligand binding site (InterPro:IPR019793), Haem peroxidase, plant/fungal/bacterial (InterPro:IPR002016), Peroxidase, active site (InterPro:IPR019794); BEST Arabidopsis thaliana protein match is: ascorbate peroxidase 2 (TAIR:AT3G09640.2); Has 8811 Blast hits to 8057 proteins in 1265 species: Archae - 75; Bacteria - 2912; Metazoa - 21; Fungi - 794; Plants - 3291; Viruses - 0; Other Eukaryotes - 1718 (source: NCBI BLink).

NP_001117244.1

ascorbate peroxidase 1 (APX1); FUNCTIONS IN: L-ascorbate peroxidase activity; INVOLVED IN: response to reactive oxygen species, response to salt stress, response to heat, hydrogen peroxide catabolic process, embryo development ending in seed dormancy; LOCATED IN: cytosol, chloroplast, plasma membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Haem peroxidase (InterPro:IPR010255), Plant ascorbate peroxidase (InterPro:IPR002207), Peroxidases heam-ligand binding site (InterPro:IPR019793), Haem peroxidase, plant/fungal/bacterial (InterPro:IPR002016), Peroxidase, active site (InterPro:IPR019794); BEST Arabidopsis thaliana protein match is: ascorbate peroxidase 2 (TAIR:AT3G09640.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

NP_001318949.1

ascorbate peroxidase 1 (APX1); FUNCTIONS IN: L-ascorbate peroxidase activity; INVOLVED IN: in 6 processes; LOCATED IN: cytosol, cell wall, chloroplast, plasma membrane; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Haem peroxidase (InterPro:IPR010255), Plant ascorbate peroxidase (InterPro:IPR002207), Peroxidases heam-ligand binding site (InterPro:IPR019793), Haem peroxidase, plant/fungal/bacterial (InterPro:IPR002016), Peroxidase, active site (InterPro:IPR019794); BEST Arabidopsis thaliana protein match is: ascorbate peroxidase 2 (TAIR:AT3G09640.2); Has 8811 Blast hits to 8057 proteins in 1265 species: Archae - 75; Bacteria - 2912; Metazoa - 21; Fungi - 794; Plants - 3291; Viruses - 0; Other Eukaryotes - 1718 (source: NCBI BLink).

NP_172267.1

ascorbate peroxidase 1 (APX1); FUNCTIONS IN: L-ascorbate peroxidase activity; INVOLVED IN: in 6 processes; LOCATED IN: cytosol, cell wall, chloroplast, plasma membrane, chloroplast stroma; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Haem peroxidase (InterPro:IPR010255), Plant ascorbate peroxidase (InterPro:IPR002207), Peroxidases heam-ligand binding site (InterPro:IPR019793), Peroxidase, active site (InterPro:IPR019794), Haem peroxidase, plant/fungal/bacterial (InterPro:IPR002016); BEST Arabidopsis thaliana protein match is: ascorbate peroxidase 2 (TAIR:AT3G09640.2); Has 8811 Blast hits to 8057 proteins in 1265 species: Archae - 75; Bacteria - 2912; Metazoa - 21; Fungi - 794; Plants - 3291; Viruses - 0; Other Eukaryotes - 1718 (source: NCBI BLink).

NP_849607.1

ascorbate peroxidase 1 (APX1); FUNCTIONS IN: L-ascorbate peroxidase activity; INVOLVED IN: in 6 processes; LOCATED IN: cytosol, cell wall, chloroplast, plasma membrane; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Haem peroxidase (InterPro:IPR010255), Plant ascorbate peroxidase (InterPro:IPR002207), Peroxidases heam-ligand binding site (InterPro:IPR019793), Peroxidase, active site (InterPro:IPR019794), Haem peroxidase, plant/fungal/bacterial (InterPro:IPR002016); BEST Arabidopsis thaliana protein match is: ascorbate peroxidase 2 (TAIR:AT3G09640.2); Has 8837 Blast hits to 8083 proteins in 1272 species: Archae - 75; Bacteria - 2918; Metazoa - 21; Fungi - 814; Plants - 3291; Viruses - 0; Other Eukaryotes - 1718 (source: NCBI BLink).

NP_973786.1

ascorbate peroxidase 1 (APX1); FUNCTIONS IN: L-ascorbate peroxidase activity; INVOLVED IN: in 6 processes; LOCATED IN: cytosol, cell wall, chloroplast, plasma membrane; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Haem peroxidase (InterPro:IPR010255), Plant ascorbate peroxidase (InterPro:IPR002207), Peroxidases heam-ligand binding site (InterPro:IPR019793), Peroxidase, active site (InterPro:IPR019794), Haem peroxidase, plant/fungal/bacterial (InterPro:IPR002016); BEST Arabidopsis thaliana protein match is: ascorbate peroxidase 2 (TAIR:AT3G09640.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).