GLU1 glutamate synthase 1 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: GLU1
Official Full Name: glutamate synthase 1
Primary source: TAIR:AT5G04140
Locus tag: AT5G04140
See related: Araport:AT5G04140
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: F21E1.60; F21E1_60; FD-GOGAT; FERREDOXIN-DEPENDENT GLUTAMATE SYNTHASE; FERREDOXIN-DEPENDENT GLUTAMATE SYNTHASE 1; GLS1; GLUS; GLUTAMATE SYNTHASE; glutamate synthase 1; GLUTAMATE SYNTHASE 1
Summary: Encodes a gene whose sequence is similar to ferredoxin dependent glutamate synthase (Fd-GOGAT). Expression in leaves is induced by light and sucrose. Proposed to be involved in photorespiration and nitrogen assimilation.
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Genomic context

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See GLU1 in Genome Data Viewer

Location:: chromosome: 5

Exon count:: 33

Sequence:: Chromosome: 5; NC_003076.8 (1129785..1138488)

Chromosome 5 - NC_003076.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

In a large-scale screen for mutants affected in leaf morphology, orbiculata1 (orb1) mutants were isolated. They exhibit a pale green phenotype and reduced growth. ORB1 encodes the GLUTAMATE SYNTHASE 1, also known as FERREDOXIN-DEPENDENT GLUTAMINE OXOGLUTARATE AMINOTRANSFERASE 1. RNA-seq profiling of orb1 mutant leaves show how nitrogen-related metabolic pathways are regulated during the vegetative phase.
Title: Deficient glutamate biosynthesis triggers a concerted upregulation of ribosomal protein genes in Arabidopsis.
glu1-2 mutant transcriptome was characterised by an extensive secondary response including the downregulation of photosynthesis-related pathways and the induction of genes and pathways involved in the plant response to a multitude of stresses.
Title: Transcriptional profiling of an Fd-GOGAT1/GLU1 mutant in Arabidopsis thaliana reveals a multiple stress response and extensive reprogramming of the transcriptome.
GLU1 interacts with SHMT1 and this interaction is necessary for photorespiratory serine hydroxymethyltransferase (SHMT) activity.
Title: Arabidopsis photorespiratory serine hydroxymethyltransferase activity requires the mitochondrial accumulation of ferredoxin-dependent glutamate synthase.
Expression of GLU1 in mesophyll cells implies that Fd-GOGAT assimilates photorespiratory and primary ammonium. GLU1 expression in vascular cells indicates that Fd-GOGAT provides amino acids for nitrogen translocation.
Title: Expression of a ferredoxin-dependent glutamate synthase gene in mesophyll and vascular cells and functions of the enzyme in ammonium assimilation in Nicotiana tabacum (L.).

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_120496.3

Supported by ESTs:

NM_180432.2

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables enzyme activator activity	IDA Inferred from Direct Assay more info	PubMed
enables glutamate synthase (ferredoxin) activity	IDA Inferred from Direct Assay more info	PubMed
enables glutamate synthase (ferredoxin) activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in L-glutamate biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within photorespiration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within photorespiration	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within response to light stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to light stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to sucrose	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in apoplast	HDA more info	PubMed
located_in chloroplast	HDA more info	PubMed
located_in chloroplast	IDA Inferred from Direct Assay more info	PubMed
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in chloroplast envelope	HDA more info	PubMed
located_in chloroplast stroma	HDA more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	HDA more info	PubMed

General protein information

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Preferred Names: glutamate synthase 1

NP_568134.1

glutamate synthase 1 (GLU1); FUNCTIONS IN: protein binding, glutamate synthase (ferredoxin) activity; INVOLVED IN: positive regulation of glycine hydroxymethyltransferase activity, response to light stimulus, photorespiration; LOCATED IN: in 6 components; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Glutamine amidotransferase, class-II (InterPro:IPR000583), Aldolase-type TIM barrel (InterPro:IPR013785), Glutamate synthase, alpha subunit, C-terminal (InterPro:IPR002489), Glutamate synthase, central-N (InterPro:IPR006982), Glutamate synthase, central-C (InterPro:IPR002932), Glutamine amidotransferase, type II (InterPro:IPR017932); BEST Arabidopsis thaliana protein match is: glutamate synthase 2 (TAIR:AT2G41220.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

NP_850763.1

glutamate synthase 1 (GLU1); FUNCTIONS IN: protein binding, glutamate synthase (ferredoxin) activity; INVOLVED IN: response to light stimulus, photorespiration; LOCATED IN: apoplast, chloroplast, membrane, chloroplast envelope; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Glutamine amidotransferase, class-II (InterPro:IPR000583), Aldolase-type TIM barrel (InterPro:IPR013785), Glutamate synthase, alpha subunit, C-terminal (InterPro:IPR002489), Glutamate synthase, central-N (InterPro:IPR006982), Glutamate synthase, central-C (InterPro:IPR002932), Glutamine amidotransferase, type II (InterPro:IPR017932); BEST Arabidopsis thaliana protein match is: glutamate synthase 2 (TAIR:AT2G41220.1); Has 17415 Blast hits to 17302 proteins in 2024 species: Archae - 267; Bacteria - 5686; Metazoa - 111; Fungi - 160; Plants - 179; Viruses - 0; Other Eukaryotes - 11012 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003076.8 Reference assembly

Range

1129785..1138488

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_120496.3 → NP_568134.1 glutamate synthase 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_568134.1

Status: REVIEWED

UniProtKB/Swiss-Prot

P93649, Q9ZNZ7

UniProtKB/TrEMBL

A0A178UNU3, A0A5S9Y233

Conserved Domains (1) summary

PRK11750
Location:102 → 1597

gltB; glutamate synthase subunit alpha; Provisional
NM_180432.2 → NP_850763.1 glutamate synthase 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_850763.1

Status: REVIEWED

UniProtKB/TrEMBL

Q540F4

Conserved Domains (5) summary

PRK11750
Location:128 → 1623

gltB; glutamate synthase subunit alpha; Provisional

cd00982
Location:1373 → 1623

gltB_C; gltb_C. This domain is found at the C-terminus of the large subunit (gltB) of glutamate synthase (GltS). GltS encodes a complex iron-sulfur flavoprotein that catalyzes the synthesis of L-glutamate from L-glutamine and 2-oxoglutarate. It requires the ...

cd02808
Location:935 → 1330

GltS_FMN; Glutamate synthase (GltS) FMN-binding domain. GltS is a complex iron-sulfur flavoprotein that catalyzes the reductive synthesis of L-glutamate from 2-oxoglutarate and L-glutamine via intramolecular channelling of ammonia, a reaction in the plant, yeast ...

pfam00310
Location:132 → 554

GATase_2; Glutamine amidotransferases class-II

pfam04898
Location:580 → 874

Glu_syn_central; Glutamate synthase central domain