CARA carbamoyl phosphate synthetase A [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: CARA
Official Full Name: carbamoyl phosphate synthetase A
Primary source: TAIR:AT3G27740
Locus tag: AT3G27740
See related: Araport:AT3G27740
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: carbamoyl phosphate synthetase A; CARBAMOYL PHOSPHATE SYNTHETASE SMALL SUBUNIT; VEN6; VENOSA 6
Summary: Encodes carbamoyl phosphate synthetase (CPS) small subunit (carA), also named as VEN6. Heterologous expression of the Arabidopsis VEN3 and VEN6 genes in a CPS-deficient Escherichia coli strain fully restored bacterial growth in minimal medium, demonstrating the enzymatic activity of the VEN3 and VEN6 proteins.
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Genomic context

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See CARA in Genome Data Viewer

Location:: chromosome: 3

Exon count:: 10

Sequence:: Chromosome: 3; NC_003074.8 (10281230..10283935, complement)

Chromosome 3 - NC_003074.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003074.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The VEN3 and VEN6 genes encode subunits of Arabidopsis carbamoyl phosphate synthetase (CPS), which is assumed to be required for the conversion of ornithine into citrulline in arginine biosynthesis.
Title: Analysis of ven3 and ven6 reticulate mutants reveals the importance of arginine biosynthesis in Arabidopsis leaf development.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_001084749.1

Supported by ESTs:

NM_113690.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	IDA Inferred from Direct Assay more info	PubMed
enables carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in 'de novo' UMP biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in 'de novo' pyrimidine nucleobase biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in L-arginine biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cellular response to phosphate starvation	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
part_of carbamoyl-phosphate synthase complex	IDA Inferred from Direct Assay more info	PubMed
located_in chloroplast	HDA more info	PubMed
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in chloroplast stroma	HDA more info	PubMed
NOT located_in cytosol	RCA inferred from Reviewed Computational Analysis more info	PubMed

General protein information

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Preferred Names: carbamoyl phosphate synthetase A

NP_001078218.1

carbamoyl phosphate synthetase A (CARA); FUNCTIONS IN: carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity, catalytic activity; INVOLVED IN: cellular response to phosphate starvation; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glutamine amidotransferase class-I, C-terminal (InterPro:IPR000991), Glutamine amidotransferase superfamily (InterPro:IPR011702), Carbamoyl phosphate synthase, GATase domain (InterPro:IPR001317), Carbamoyl phosphate synthase, small subunit, N-terminal (InterPro:IPR002474), Glutamine amidotransferase type 1 (InterPro:IPR017926), Carbamoyl phosphate synthase, small subunit (InterPro:IPR006274), Anthranilate synthase component II/delta crystallin (InterPro:IPR006220); BEST Arabidopsis thaliana protein match is: anthranilate synthase beta subunit 1 (TAIR:AT1G25220.1); Has 28262 Blast hits to 23423 proteins in 4115 species: Archae - 591; Bacteria - 17320; Metazoa - 1741; Fungi - 716; Plants - 245; Viruses - 0; Other Eukaryotes - 7649 (source: NCBI BLink).

NP_566824.1

carbamoyl phosphate synthetase A (CARA); FUNCTIONS IN: carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity, catalytic activity; INVOLVED IN: cellular response to phosphate starvation; LOCATED IN: chloroplast, chloroplast stroma; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glutamine amidotransferase class-I, C-terminal (InterPro:IPR000991), Glutamine amidotransferase superfamily (InterPro:IPR011702), Carbamoyl phosphate synthase, GATase domain (InterPro:IPR001317), Carbamoyl phosphate synthase, small subunit, N-terminal (InterPro:IPR002474), Glutamine amidotransferase type 1 (InterPro:IPR017926), Carbamoyl phosphate synthase, small subunit (InterPro:IPR006274), Anthranilate synthase component II/delta crystallin (InterPro:IPR006220); BEST Arabidopsis thaliana protein match is: anthranilate synthase beta subunit 1 (TAIR:AT1G25220.1); Has 23531 Blast hits to 23511 proteins in 4128 species: Archae - 520; Bacteria - 13864; Metazoa - 1675; Fungi - 510; Plants - 198; Viruses - 0; Other Eukaryotes - 6764 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003074.8 Reference assembly

Range

10281230..10283935 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_113690.3 → NP_566824.1 carbamoyl phosphate synthetase A [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_566824.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O24447, Q9LVW7

UniProtKB/TrEMBL

A0A178VAM7, A0A7G2ENW7

Conserved Domains (1) summary

PLN02771
Location:1 → 416

PLN02771; carbamoyl-phosphate synthase (glutamine-hydrolyzing)
NM_001084749.1 → NP_001078218.1 carbamoyl phosphate synthetase A [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001078218.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A7G2ENW7, A8MRD8

Conserved Domains (3) summary

cd01744
Location:172 → 348

GATase1_CPSase; Small chain of the glutamine-dependent form of carbamoyl phosphate synthase, CPSase II

PLN02771
Location:1 → 344

PLN02771; carbamoyl-phosphate synthase (glutamine-hydrolyzing)

pfam00988
Location:58 → 122

CPSase_sm_chain; Carbamoyl-phosphate synthase small chain, CPSase domain