Slc20a1 solute carrier family 20 member 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Slc20a1provided by RGD
Official Full Name: solute carrier family 20 member 1provided by RGD
Primary source: RGD:621079
See related: EnsemblRapid:ENSRNOG00000018567 AllianceGenome:RGD:621079
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: PiT-1
Summary: Enables high-affinity inorganic phosphate:sodium symporter activity. Involved in phosphate ion transport and sodium ion transport. Is integral component of membrane. Orthologous to human SLC20A1 (solute carrier family 20 member 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Brain (RPKM 286.2), Spleen (RPKM 255.3) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 3q36

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	3	NC_086021.1 (136880360..136894255)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	3	NC_051338.1 (116427095..116441049)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	3	NC_005102.4 (121725204..121739160)

Chromosome 3 - NC_086021.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

mechanism involving TRPM7 and Pit-1 underpins the magnesium-mediated reversal of high-phosphate-associated vascular calcification
Title: Magnesium prevents phosphate-induced vascular calcification via TRPM7 and Pit-1 in an aortic tissue culture model.
Apelin ameliorates VC by suppressing osteoblastic differentiation of VSMCs through downregulation of Pit-1.
Title: Apelin: A novel inhibitor of vascular calcification in chronic kidney disease.
NaPi-III mRNA expression is affected by hyperphosphatemia.
Title: Effect of hyperphosphatemia on gene expression of the Na-Pi cotransporter in rats.
Incisor enamel formation is impaired in transgenic rats overexpressing the type III NaPi transporter Slc20a1.
Title: Incisor enamel formation is impaired in transgenic rats overexpressing the type III NaPi transporter Slc20a1.
Dexamethasone and cyclic AMP regulate sodium phosphate cotransporter (NaPi-IIb and Pit-1) mRNA and phosphate uptake in rat alveolar type II epithelial cells.
Title: Dexamethasone and cyclic AMP regulate sodium phosphate cotransporter (NaPi-IIb and Pit-1) mRNA and phosphate uptake in rat alveolar type II epithelial cells.
phosphate uptake through the type III sodium-dependent phosphate cotransporter, Pit-1, leads to induction of apoptosis and subsequent calcification of VSMC.
Title: Mechanism of phosphate-induced calcification in rat aortic tissue culture: possible involvement of Pit-1 and apoptosis.
Pi transport across the plasma membrane does not correlate directly with calcification, but the expression of Pit-1 in the ER opens new possibilities for the study of the pathogenesis of vascular calcification.
Title: Vascular smooth muscle cell calcification and SLC20 inorganic phosphate transporters: effects of PDGF, TNF-alpha, and Pi.
Osteoblast autonomous Pi regulation via PiT-1 plays a role in bone mineralization.
Title: Osteoblast autonomous Pi regulation via Pit1 plays a role in bone mineralization.
Sodium-phosphate cotransporter (PiT-1) soleus and red gastrocnemius was similar and seven- to eightfold greater than PiT-1 expression in white gastrocnemius. PiT-1 expression did not match pattern of P(i) uptake across fiber types.
Title: Phosphate uptake and PiT-1 protein expression in rat skeletal muscle.
We have used RNA interference to demonstrate that Pit1 and Pit2 are the transporters responsible for Na-dependent Pi transport in VSMCs.
Title: Characterization of phosphate transport in rat vascular smooth muscle cells: implications for vascular calcification.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH138625 (e-PCR)
- UniSTS:220712

Slc10a1 (e-PCR), detects polymorphism
- UniSTS:528184

Slc10a1 (e-PCR), detects polymorphism
- UniSTS:528185

RH128315 (e-PCR)
- UniSTS:211623

AA963219 (e-PCR)
- UniSTS:248006

Slc20a1 (e-PCR), detects polymorphism
- UniSTS:547018

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables high-affinity phosphate:sodium symporter activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphate transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables sodium:phosphate symporter activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within biomineral tissue development	ISO Inferred from Sequence Orthology more info
involved_in cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in cell population proliferation	ISS Inferred from Sequence or Structural Similarity more info
involved_in phosphate ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphate ion transport	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info	PubMed
involved_in sodium ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in sodium ion transport	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: sodium-dependent phosphate transporter 1

Names: RPHO-1; phosphate transporter 1; solute carrier family 20 (phosphate transporter), member 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031148.3 → NP_112410.1 sodium-dependent phosphate transporter 1

See identical proteins and their annotated locations for NP_112410.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000003

UniProtKB/Swiss-Prot

Q9JJP0

UniProtKB/TrEMBL

A6HQ55

Conserved Domains (1) summary

pfam01384
Location:508 → 667

PHO4; Phosphate transporter family

RNA

NR_197219.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

JAXUCZ010000003
NR_197220.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

JAXUCZ010000003

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086021.1 Reference GRCr8

Range

136880360..136894255

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006234991.5 → XP_006235053.1 sodium-dependent phosphate transporter 1 isoform X1

Related

ENSRNOP00000025294.4, ENSRNOT00000025294.8

Conserved Domains (1) summary

pfam01384
Location:508 → 667

PHO4; Phosphate transporter family
XM_006234992.5 → XP_006235054.1 sodium-dependent phosphate transporter 1 isoform X4

Conserved Domains (2) summary

COG0306
Location:25 → 214

PitA; Phosphate/sulfate permease [Inorganic ion transport and metabolism]

pfam01384
Location:46 → 148

PHO4; Phosphate transporter family
XM_039105927.2 → XP_038961855.1 sodium-dependent phosphate transporter 1 isoform X2

Conserved Domains (1) summary

pfam01384
Location:291 → 450

PHO4; Phosphate transporter family
XM_039105928.1 → XP_038961856.1 sodium-dependent phosphate transporter 1 isoform X3

Conserved Domains (1) summary

pfam01384
Location:251 → 410

PHO4; Phosphate transporter family