Dgkz diacylglycerol kinase zeta [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Dgkzprovided by RGD
Official Full Name: diacylglycerol kinase zetaprovided by RGD
Primary source: RGD:70929
See related: EnsemblRapid:ENSRNOG00000017737 AllianceGenome:RGD:70929
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables diacylglycerol kinase activity. Predicted to be involved in several processes, including glycerolipid metabolic process; lipid phosphorylation; and maintenance of postsynaptic density structure. Predicted to act upstream of or within negative regulation of Ras protein signal transduction. Located in nucleus. Is active in glutamatergic synapse and postsynaptic density. Orthologous to human DGKZ (diacylglycerol kinase zeta). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Thymus (RPKM 437.4), Brain (RPKM 234.6) and 8 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 3q24

Exon count:: 35

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	3	NC_086021.1 (98359804..98401847, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	3	NC_051338.1 (77904149..77946114, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	3	NC_005102.4 (80844002..80886487, complement)

Chromosome 3 - NC_086021.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The expression of diacylglycerol kinase isoforms alpha and zeta correlates with the progression of experimental autoimmune encephalomyelitis in rats.
Title: The expression of diacylglycerol kinase isoforms α and ζ correlates with the progression of experimental autoimmune encephalomyelitis in rats.
The results suggest that DGKzeta cytoplasmic translocation in neurons under hypoxic stress is regulated by some mechanism which differs from that mediated by NAP1-like proteins.
Title: Cellular expression and localization of DGKζ-interacting NAP1-like proteins in the brain and functional implications under hypoxic stress.
The DGKzeta and DGKalpha localize predominantly to the nucleus of ganglion cells.
Title: Distinct expression and localization of diacylglycerol kinase isozymes in rat retina.
The results show that DGKzeta cytoplasmic translocation is triggered by activation of NMDAR with subsequent extracellular Ca(2+) influx.
Title: NMDA receptor-mediated Ca(2+) influx triggers nucleocytoplasmic translocation of diacylglycerol kinase ζ under oxygen-glucose deprivation conditions, an in vitro model of ischemia, in rat hippocampal slices.
Results suggest that DGKzeta is involved in the acute phase of cellular response to ischemic stress in cardiomyocytes
Title: Nuclear relocation of DGKζ in cardiomyocytes under conditions of ischemia/reperfusion.
within the cardiomyocyte, there exists a PPAR-DGK signalling axis that underpins the ability of PPAR ligands to inhibit ET1-dependent hypertrophy
Title: Suppression of endothelin-1-induced cardiac myocyte hypertrophy by PPAR agonists: role of diacylglycerol kinase zeta.
Cytoplasmic translocation of DGKzeta might be involved in early events of the apoptotic cell death pathway in hippocampal neurons under stressed conditions
Title: Excitotoxicity by kainate-induced seizure causes diacylglycerol kinase ζ to shuttle from the nucleus to the cytoplasm in hippocampal neurons.
DGKzeta is induced in activated microglia in brain trauma, suggesting the functional significance of DGKzeta in this process.
Title: Brain trauma induces expression of diacylglycerol kinase zeta in microglia.
Results show that diacylglycerol kinase zeta is targeted to excitatory synapses through its direct interaction with the postsynaptic PDZ scaffold PSD-95.
Title: Synaptic removal of diacylglycerol by DGKzeta and PSD-95 regulates dendritic spine maintenance.
DGK zeta is primarily a nuclear protein in neurons, it can also be cytoplasmic in some conditions, and the subcellular location depends not only on the cell type but also on the developmental state or growth conditions of the cell.
Title: Nuclear localization of diacylglycerol kinase zeta in neurons.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP-dependent diacylglycerol kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP-dependent diacylglycerol kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent diacylglycerol kinase activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent diacylglycerol kinase activity	ISO Inferred from Sequence Orthology more info
enables alkylglycerol kinase activity	IEA Inferred from Electronic Annotation more info
enables enzyme inhibitor activity	ISO Inferred from Sequence Orthology more info
enables kinase activity	ISO Inferred from Sequence Orthology more info
enables lipid kinase activity	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of Ras protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in diacylglycerol metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in diacylglycerol metabolic process	IEA Inferred from Electronic Annotation more info
involved_in diacylglycerol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in diacylglycerol metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in glycerolipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in glycerolipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in lipid phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in lipid phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in maintenance of postsynaptic density structure	ISO Inferred from Sequence Orthology more info
involved_in mitotic G1 DNA damage checkpoint signaling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of Ras protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of T cell receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of T cell receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in phosphatidic acid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidic acid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidic acid biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidic acid biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in phosphorylation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in Schaffer collateral - CA1 synapse	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
is_active_in glutamatergic synapse	IBA Inferred from Biological aspect of Ancestor more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	IEA Inferred from Electronic Annotation more info
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
is_active_in postsynaptic density	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: diacylglycerol kinase zeta

Names: 104 kDa diacylglycerol kinase; DAG kinase zeta; DGK-IV; DGK-zeta; diglyceride kinase zeta

NP_001402081.1

EC 2.7.1.107

EC 2.7.1.93

NP_112405.1

EC 2.7.1.107

EC 2.7.1.93

XP_006234656.1

EC 2.7.1.107

EC 2.7.1.93

XP_017447551.1

EC 2.7.1.107

EC 2.7.1.93

XP_038961852.1

EC 2.7.1.107

EC 2.7.1.93

XP_038961854.1

EC 2.7.1.107

EC 2.7.1.93

XP_063140712.1

EC 2.7.1.107

EC 2.7.1.93

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001415152.1 → NP_001402081.1 diacylglycerol kinase zeta isoform 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000003

UniProtKB/TrEMBL

A0A0G2K707

Related

ENSRNOP00000074012.2, ENSRNOT00000091265.3
NM_001429512.1 → NP_001416441.1 diacylglycerol kinase zeta isoform 3

Status: VALIDATED

Source sequence(s)

JAXUCZ010000003

UniProtKB/TrEMBL

A0A8I6A444, A6HNF2
NM_001429513.1 → NP_001416442.1 diacylglycerol kinase zeta isoform 4

Status: VALIDATED

Source sequence(s)

JAXUCZ010000003

UniProtKB/TrEMBL

A0A8I6GM44
NM_031143.3 → NP_112405.1 diacylglycerol kinase zeta isoform 2

See identical proteins and their annotated locations for NP_112405.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000003

UniProtKB/Swiss-Prot

O08560

UniProtKB/TrEMBL

A0A8I6AMI5, A6HNF1

Related

ENSRNOP00000024280.2, ENSRNOT00000024280.6

Conserved Domains (7) summary

smart00045
Location:447 → 604

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:298 → 419

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG1597
Location:294 → 617

LCB5; Diacylglycerol kinase family enzyme [Lipid transport and metabolism, General function prediction only]

cd00204
Location:795 → 911

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam12796
Location:795 → 889

Ank_2; Ankyrin repeats (3 copies)

sd00045
Location:795 → 821

ANK; ANK repeat [structural motif]

cl00040
Location:173 → 232

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086021.1 Reference GRCr8

Range

98359804..98401847 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017592062.3 → XP_017447551.1 diacylglycerol kinase zeta isoform X3

Related

ENSRNOP00000097565.1, ENSRNOT00000117598.2
XM_039105926.2 → XP_038961854.1 diacylglycerol kinase zeta isoform X4

UniProtKB/TrEMBL

A0A8I6AMI5

Conserved Domains (6) summary

smart00045
Location:327 → 484

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:178 → 299

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

sd00045
Location:675 → 701

ANK; ANK repeat [structural motif]

pfam12796
Location:675 → 769

Ank_2; Ankyrin repeats (3 copies)

cd20895
Location:51 → 125

C1_DGKzeta_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase zeta (DAG kinase zeta) and similar proteins

cl00040
Location:1 → 41

C1; protein kinase C conserved region 1 (C1 domain) superfamily
XM_006234594.5 → XP_006234656.1 diacylglycerol kinase zeta isoform X1

Related

ENSRNOP00000087400.1, ENSRNOT00000095998.2

Conserved Domains (7) summary

smart00045
Location:639 → 796

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:490 → 611

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

COG1597
Location:486 → 809

LCB5; Diacylglycerol kinase family enzyme [Lipid transport and metabolism, General function prediction only]

cd00204
Location:987 → 1103

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam12796
Location:987 → 1081

Ank_2; Ankyrin repeats (3 copies)

sd00045
Location:987 → 1013

ANK; ANK repeat [structural motif]

cl00040
Location:365 → 424

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...
XM_063284642.1 → XP_063140712.1 diacylglycerol kinase zeta isoform X1

UniProtKB/TrEMBL

A0A8I6A444, A6HNF2
XM_039105924.2 → XP_038961852.1 diacylglycerol kinase zeta isoform X2

Conserved Domains (4) summary

smart00045
Location:639 → 796

DAGKa; Diacylglycerol kinase accessory domain (presumed)

smart00046
Location:490 → 611

DAGKc; Diacylglycerol kinase catalytic domain (presumed)

cd20849
Location:280 → 353

C1_DGKzeta_rpt1; first protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase zeta (DAG kinase zeta) and similar proteins

cd20895
Location:363 → 437

C1_DGKzeta_rpt2; second protein kinase C conserved region 1 (C1 domain) found in diacylglycerol kinase zeta (DAG kinase zeta) and similar proteins