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PHO2 phosphate 2 [ Arabidopsis thaliana (thale cress) ]

Gene ID: 817943, updated on 18-Sep-2024

Summary

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Official Symbol
PHO2
Official Full Name
phosphate 2
Primary source
TAIR:AT2G33770
Locus tag
AT2G33770
See related
Araport:AT2G33770
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Arabidopsis thaliana (ecotype: Columbia)
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as
ATUBC24; phosphate 2; T1B8.8; T1B8_8; UBC24; UBIQUITIN-CONJUGATING ENZYME 24
Summary
Encodes a ubiquitin-conjugating E2 enzyme. UBC24 mRNA accumulation is suppressed by miR399f, miR399b and miR399c. Involved in phosphate starvation response and mediates degradation of PHO1 and PHT1s at endomembrane.
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Genomic context

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See PHO2 in Genome Data Viewer
Location:
chromosome: 2
Exon count:
9
Sequence:
Chromosome: 2; NC_003071.7 (14277558..14283113, complement)

Chromosome 2 - NC_003071.7Genomic Context describing neighboring genes Neighboring gene Nitrogen regulatory PII-like, alpha/beta Neighboring gene purine permease 2 Neighboring gene Pentatricopeptide repeat (PPR) superfamily protein Neighboring gene miscRNA Neighboring gene ncRNA Neighboring gene ralf-like 19

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Regulatory network of microRNA399 and PHO2 by systemic signaling. Lin SI, et al. Plant Physiol, 2008 Jun. PMID 18390805, Free PMC Article
  2. pho2, a phosphate overaccumulator, is caused by a nonsense mutation in a microRNA399 target gene. Aung K, et al. Plant Physiol, 2006 Jul. PMID 16679417, Free PMC Article
  3. Uptake and translocation of phosphate by pho2 mutant and wild-type seedlings of Arabidopsis thaliana. Dong B, et al. Planta, 1998 Jun. PMID 9637070
  4. Regulation of stomatal development by stomatal lineage miRNAs. Zhu J, et al. Proc Natl Acad Sci U S A, 2020 Mar 17. PMID 32123075, Free PMC Article
  5. NITROGEN LIMITATION ADAPTATION recruits PHOSPHATE2 to target the phosphate transporter PT2 for degradation during the regulation of Arabidopsis phosphate homeostasis. Park BS, et al. Plant Cell, 2014 Jan. PMID 24474629, Free PMC Article

See all (14) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Study shows that miR399-mediated PHO2 regulation, involved in phosphate homeostasis, contributes to the control of stomatal development in Arabidopsis.
    Title: Regulation of stomatal development by stomatal lineage miRNAs.
  2. PHR1 is a key factor for metabolic reprogramming during phosphorus (P) limitation. The effects of pho2 or microRNA399 overexpression were comparatively minor.
    Title: Identification of primary and secondary metabolites with phosphorus status-dependent abundance in Arabidopsis, and of the transcription factor PHR1 as a major regulator of metabolic changes during phosphorus limitation.
  3. NLA is an E3 ligase that specifically requires UBC24 for polyubiquitination in Arabidopsis thaliana.
    Title: NITROGEN LIMITATION ADAPTATION recruits PHOSPHATE2 to target the phosphate transporter PT2 for degradation during the regulation of Arabidopsis phosphate homeostasis.
  4. PHO2 negatively regulates the protein abundance of PHF1 and PHT1 proteins under Pi-sufficient conditions
    Title: Identification of downstream components of ubiquitin-conjugating enzyme PHOSPHATE2 by quantitative membrane proteomics in Arabidopsis roots.
  5. The phosphate-accumulating mutants siz1 and pho2 reduce the uptake and accumulation of Na(+), leading to enhanced salt tolerance, and that, genetically, SIZ1 and PHO2 are likely independent of SOS3-dependent salt signaling.
    Title: Increased tolerance to salt stress in the phosphate-accumulating Arabidopsis mutants siz1 and pho2.
  6. PHO2-dependent degradation of PHO1 modulates phosphate homeostasis in Arabidopsis.
    Title: PHO2-dependent degradation of PHO1 modulates phosphate homeostasis in Arabidopsis.
  7. Expression of TSF was increased in miR399b-overexpressing plants and pho2 mutants at 23 degrees C, suggesting that their early flowering phenotype is associated with TSF upregulation.
    Title: The role of the miR399-PHO2 module in the regulation of flowering time in response to different ambient temperatures in Arabidopsis thaliana.
  8. IPS1 overexpression results in increased accumulation of the miR-399 target PHO2 mRNA and, concomitantly, in reduced shoot Pi content; engineering of IPS1 to be cleavable abolishes its inhibitory activity on miR-399.
    Title: Target mimicry provides a new mechanism for regulation of microRNA activity.
  9. Map-based cloning identified PHO2 as At2g33770. miR399 and PHO2 are in a branch of the Pi-signaling network downstream of PHR1.
    Title: PHO2, microRNA399, and PHR1 define a phosphate-signaling pathway in plants.
  10. A previously identified Pi overaccumulator, pho2, is caused by a single nucleotide mutation resulting in early termination within the UBC24 gene.
    Title: pho2, a phosphate overaccumulator, is caused by a nonsense mutation in a microRNA399 target gene.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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NM_179887.3

Gene Ontology Provided by TAIR

Function Evidence Code Pubs
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ubiquitin conjugating enzyme activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin-protein transferase activity ISS
Inferred from Sequence or Structural Similarity
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within cellular response to phosphate starvation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within phosphate ion homeostasis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within phosphate ion transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein ubiquitination IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within regulation of phosphate transmembrane transport IGI
Inferred from Genetic Interaction
more info
 PubMed 
Component Evidence Code Pubs
located_in Golgi apparatus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endoplasmic reticulum IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus ISM
Inferred from Sequence Model
more info
  

General protein information

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Preferred Names
phosphate 2
NP_850218.1
  • phosphate 2 (PHO2); CONTAINS InterPro DOMAIN/s: Ubiquitin-conjugating enzyme/RWD-like (InterPro:IPR016135), Ubiquitin-conjugating enzyme, E2 (InterPro:IPR000608); BEST Arabidopsis thaliana protein match is: ubiquitin-conjugating enzyme 23 (TAIR:AT2G16920.1); Has 6405 Blast hits to 6302 proteins in 368 species: Archae - 0; Bacteria - 0; Metazoa - 2716; Fungi - 1263; Plants - 1493; Viruses - 17; Other Eukaryotes - 916 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003071.7 Reference assembly

    Range
    14277558..14283113 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_179887.3 → NP_850218.1  phosphate 2 [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_850218.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    P93012, Q0WLN6, Q0WM96, Q8VY10
    UniProtKB/TrEMBL
    A0A5S9X3R7, A0A654EZY1
    Conserved Domains (1) summary
    cd00195
    Location:664 → 817
    UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q8VY10.1

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