IRE1A Endoribonuclease/protein kinase IRE1-like protein [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: IRE1A
Official Full Name: Endoribonuclease/protein kinase IRE1-like protein
Primary source: TAIR:AT2G17520
Locus tag: AT2G17520
See related: Araport:AT2G17520
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS THALIANA INOSITOL REQUIRING 1-2; ATIRE1-2; AtIRE1A; INOSITOL REQUIRING 1-2; IRE1-2; MJB20.8; MJB20_8; TRANSMEMBRANE PROTEIN KINASE/RIBONUCLEASE
Summary: Encodes a endoribonuclease/protein kinase IRE1-like protein that is predicted to form a type I transmembrane protein structure and contain kinase/endoribonuclease domains at their C-terminal halves. The transcript levels for several ER-stress responsive genes, including six protein disulfide isomerases (PDIs), BiP2, and AtbZIP60 are not affected in ire1-2 null mutants.
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Genomic context

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See IRE1A in Genome Data Viewer

Location:: chromosome: 2

Exon count:: 8

Sequence:: Chromosome: 2; NC_003071.7 (7617151..7621129)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The UPR regulator IRE1 promotes balanced organ development by restricting TOR-dependent control of cellular differentiation in Arabidopsis.
Title: The UPR regulator IRE1 promotes balanced organ development by restricting TOR-dependent control of cellular differentiation in Arabidopsis.
Arabidopsis GAAPs interacting with MAPR3 modulate the IRE1-dependent pathway upon endoplasmic reticulum stress.
Title: Arabidopsis GAAPs interacting with MAPR3 modulate the IRE1-dependent pathway upon endoplasmic reticulum stress.
Results found the triple mutant of IRE1A, IRE1B, and IRE1C is lethal. The expression of a mutant form of IRE1B that lacks the luminal sensor domain (DeltaLD) complemented a developmental defect in the male gametophyte in ire1a/b/c haplotype.
Title: Unfolded protein-independent IRE1 activation contributes to multifaceted developmental processes in Arabidopsis.
Results reveal the important role of IRE1A in plant growth and development, and BLI negatively controls IRE1A's function under normal growth condition in plants.
Title: BLISTER-regulated vegetative growth is dependent on the protein kinase domain of ER stress modulator IRE1A in Arabidopsis thaliana.
These data suggest that ER stress-activated pathways, led by IRE1 and BI-1, respond to invading potyvirus and potexviruses to restrict virus infection and enable physiological changes enabling plants to tolerate virus assault.
Title: The IRE1/bZIP60 Pathway and Bax Inhibitor 1 Suppress Systemic Accumulation of Potyviruses and Potexviruses in Arabidopsis and Nicotiana benthamiana Plants.
An Arabidopsis mutant of a conserved endoplasmic reticulum (ER) stress sensor IRE1 exhibits defects in the auxin response and levels, which supports that the plant IRE1 is required for auxin homeostasis.
Title: Inter-regulation of the unfolded protein response and auxin signaling.
Arabidopsis AGB1, a subunit of the ubiquitous heterotrimeric GTP-binding protein family, and AtIRE1A/AtIRE1B independently control two essential plant unfolded protein response pathways.
Title: AtIRE1A/AtIRE1B and AGB1 independently control two essential unfolded protein response pathways in Arabidopsis.
observed that the active form of bZIP60 protein detected in the wild-type was missing in ire1a/ire1b
Title: Arabidopsis IRE1 catalyses unconventional splicing of bZIP60 mRNA to produce the active transcription factor.
Defects in IRE1 enhance cell death and fail to degrade mRNAs encoding secretory pathway proteins in the Arabidopsis unfolded protein response.
Title: Defects in IRE1 enhance cell death and fail to degrade mRNAs encoding secretory pathway proteins in the Arabidopsis unfolded protein response.
the IRE1/bZIP60 branch of unfolded protein response is part of the plant response to pathogens for which two AT IRE1 isoforms play only partially overlapping roles; and IRE1 has both bZIP60-dependent and bZIP60-independent functions in plant immunity.
Title: IRE1/bZIP60-mediated unfolded protein response plays distinct roles in plant immunity and abiotic stress responses.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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NM_127306.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables RNA endonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within RNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in RNA splicing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in defense response to bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within endoplasmic reticulum unfolded protein response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein autophosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within response to endoplasmic reticulum stress	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to salicylic acid	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in secretory vesicle	HDA more info	PubMed

General protein information

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Preferred Names: Endoribonuclease/protein kinase IRE1-like protein

NP_565419.1

IRE1A; CONTAINS InterPro DOMAIN/s: Protein kinase, catalytic domain (InterPro:IPR000719), KEN domain, ribonuclease activator (InterPro:IPR010513), PUG domain (InterPro:IPR006567), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271); BEST Arabidopsis thaliana protein match is: inositol requiring 1-1 (TAIR:AT5G24360.1); Has 104790 Blast hits to 103944 proteins in 4127 species: Archae - 110; Bacteria - 12550; Metazoa - 37627; Fungi - 10369; Plants - 27162; Viruses - 328; Other Eukaryotes - 16644 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003071.7 Reference assembly

Range

7617151..7621129

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_127306.4 → NP_565419.1 Endoribonuclease/protein kinase IRE1-like protein [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_565419.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q0WPT1, Q56WN0, Q9C5S2, Q9SHL6

UniProtKB/TrEMBL

A0A178VYT2, A0A5S9WYR0

Conserved Domains (3) summary

cd10422
Location:708 → 836

RNase_Ire1; RNase domain (also known as the kinase extension nuclease domain) of Ire1

cd13982
Location:412 → 705

STKc_IRE1; Catalytic domain of the Serine/Threonine kinase, Inositol-requiring protein 1

cl14874
Location:62 → 240

Luminal_IRE1_like; The Luminal domain, a dimerization domain, of Inositol-requiring protein 1-like proteins