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DUSP16 dual specificity phosphatase 16 [ Homo sapiens (human) ]

Gene ID: 80824, updated on 11-Apr-2024

Summary

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Official Symbol
DUSP16provided by HGNC
Official Full Name
dual specificity phosphatase 16provided by HGNC
Primary source
HGNC:HGNC:17909
See related
Ensembl:ENSG00000111266 MIM:607175; AllianceGenome:HGNC:17909
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
MKP7; MKP-7
Summary
This gene encodes a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. The encoded protein specifically regulates the c-Jun amino-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) pathways.[provided by RefSeq, May 2010]
Expression
Ubiquitous expression in adrenal (RPKM 19.6), liver (RPKM 13.0) and 25 other tissues See more
Orthologs
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Genomic context

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Location:
12p13.2
Exon count:
10
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 12 NC_000012.12 (12473282..12562863, complement)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 12 NC_060936.1 (12342454..12456119, complement)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 12 NC_000012.11 (12626216..12715797, complement)

Chromosome 12 - NC_000012.12Genomic Context describing neighboring genes Neighboring gene H3K27ac hESC enhancer GRCh37_chr12:12509797-12510475 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 6011 Neighboring gene loss of heterozygosity on chromosome 12, region 2 Neighboring gene BLOC-1 related complex subunit 5 Neighboring gene Sharpr-MPRA regulatory region 10643 Neighboring gene ReSE screen-validated silencer GRCh37_chr12:12560630-12560819 Neighboring gene Sharpr-MPRA regulatory region 7135 Neighboring gene MED14-independent group 3 enhancer GRCh37_chr12:12592110-12593309 Neighboring gene Neanderthal introgressed variant-containing enhancer experimental_25257 Neighboring gene adaptor related protein complex 3 subunit sigma 1 pseudogene Neighboring gene H3K4me1 hESC enhancer GRCh37_chr12:12610012-12610690 Neighboring gene H3K27ac hESC enhancer GRCh37_chr12:12686984-12687949 Neighboring gene H3K27ac hESC enhancer GRCh37_chr12:12688131-12688632 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 4254 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 6012 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 6013 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 4255 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 4256 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 4257 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 4258 Neighboring gene uncharacterized LOC107984486 Neighboring gene ribosomal protein L19 pseudogene 17 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 6014 Neighboring gene NANOG hESC enhancer GRCh37_chr12:12737991-12738492

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. A novel site on dual-specificity phosphatase MKP7/DUSP16 is required for catalysis and MAPK binding. Shillingford S, et al. J Biol Chem, 2022 Dec. PMID 36272649, Free PMC Article
  2. DUSP16 promotes cancer chemoresistance through regulation of mitochondria-mediated cell death. Low HB, et al. Nat Commun, 2021 Apr 16. PMID 33863904, Free PMC Article
  3. DUSP16 is a regulator of human hematopoietic stem and progenitor cells and promotes their expansion ex vivo. Wang X, et al. Leukemia, 2021 May. PMID 33077868, Free PMC Article
  4. DUSP16 ablation arrests the cell cycle and induces cellular senescence. Zhang H, et al. FEBS J, 2015 Dec. PMID 26381291
  5. MKP-7, a negative regulator of JNK, regulates VCAM-1 expression through IRF-1. Nizamutdinova IT, et al. Cell Signal, 2012 Apr. PMID 22182512

See all (53) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A novel site on dual-specificity phosphatase MKP7/DUSP16 is required for catalysis and MAPK binding.
    Title: A novel site on dual-specificity phosphatase MKP7/DUSP16 is required for catalysis and MAPK binding.
  2. CircDUSP16 Contributes to Cell Development in Esophageal Squamous Cell Carcinoma by Regulating miR-497-5p/TKTL1 Axis.
    Title: CircDUSP16 Contributes to Cell Development in Esophageal Squamous Cell Carcinoma by Regulating miR-497-5p/TKTL1 Axis.
  3. DUSP16 is a regulator of human hematopoietic stem and progenitor cells and promotes their expansion ex vivo.
    Title: DUSP16 is a regulator of human hematopoietic stem and progenitor cells and promotes their expansion ex vivo.
  4. DUSP16 promotes cancer chemoresistance through regulation of mitochondria-mediated cell death.
    Title: DUSP16 promotes cancer chemoresistance through regulation of mitochondria-mediated cell death.
  5. Chemotherapy increased DUSP9 expression and decreased DUSP16 expression in a HIF1-dependent manner.
    Title: Reciprocal Regulation of DUSP9 and DUSP16 Expression by HIF1 Controls ERK and p38 MAP Kinase Activity and Mediates Chemotherapy-Induced Breast Cancer Stem Cell Enrichment.
  6. The crystal structure of JNK1 bound to the catalytic domain of MKP7 at 2.4-A resolution, providing high-resolution structural insight into the FXF-docking interaction, is reported.
    Title: A conserved motif in JNK/p38-specific MAPK phosphatases as a determinant for JNK1 recognition and inactivation.
  7. The DUSP16 ablation leads to a G1/S transition arrest, reduced incorporation of 5-bromodeoxyuridine, enhanced senescence-associated beta-galactosidase activity, and formation of senescence-associated heterochromatic foci.
    Title: DUSP16 ablation arrests the cell cycle and induces cellular senescence.
  8. analysis of the interaction of the MAPK binding domain of DUSP16 with p38alpha
    Title: Structural basis for the regulation of the mitogen-activated protein (MAP) kinase p38α by the dual specificity phosphatase 16 MAP kinase binding domain in solution.
  9. PPARdelta-mediated messenger RNA stabilization of mitogen-activated protein kinase phosphatase (MKP)-7 was responsible for the GW501516-mediated inhibition of JNK signaling.
    Title: PPARδ inhibits UVB-induced secretion of MMP-1 through MKP-7-mediated suppression of JNK signaling.
  10. Data indicate that the activities of phosphoprotein phosphatases MKP5 and MKP7 were determined in the system.
    Title: A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts.
Submit: New GeneRIF Correction See all GeneRIFs (20)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Host-microbe interactions have shaped the genetic architecture of inflammatory bowel disease.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Replication interactions

Interaction Pubs
Knockdown of dual specificity phosphatase 16 (DUSP16) by siRNA inhibits the early stages of HIV-1 replication in 293T cells infected with VSV-G pseudotyped HIV-1 PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • FLJ36298, FLJ40991, KIAA1700, MGC129701, MGC129702

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables JUN kinase binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables MAP kinase tyrosine phosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables MAP kinase tyrosine/serine/threonine phosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables mitogen-activated protein kinase binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables mitogen-activated protein kinase p38 binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables myosin phosphatase activity IEA
Inferred from Electronic Annotation
more info
  
enables phosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables phosphoprotein phosphatase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein carrier chaperone IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein tyrosine/threonine phosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in dephosphorylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of MAPK cascade IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within negative regulation of MAPK cascade IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in peptidyl-tyrosine dephosphorylation IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasmic vesicle IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol TAS
Traceable Author Statement
more info
  
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
dual specificity protein phosphatase 16
Names
MAP kinase phosphatase 7
MAPK phosphatase-7
mitogen-activated protein kinase phosphatase 7
NP_085143.1
XP_006719218.1
XP_011519158.1
XP_047285524.1
XP_047285525.1
XP_047285526.1
XP_047285527.1
XP_054187675.1
XP_054187676.1
XP_054187677.1
XP_054187678.1
XP_054187679.1
XP_054187680.1
XP_054187681.1
XP_054187682.1
XP_054187683.1
XP_054229273.1
XP_054229274.1
XP_054229275.1
XP_054229276.1
XP_054229277.1
XP_054229278.1
XP_054229279.1
XP_054229280.1
XP_054229281.1
XP_054229282.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_021402.2 RefSeqGene

    Range
    4652..94233
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_030640.3 → NP_085143.1  dual specificity protein phosphatase 16

    See identical proteins and their annotated locations for NP_085143.1

    Status: REVIEWED

    Source sequence(s)
    AC007619, AC092824, AF506796, DB094627
    Consensus CDS
    CCDS8650.1
    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3
    UniProtKB/TrEMBL
    Q32MA0, Q96N49
    Related
    ENSP00000298573.5, ENST00000298573.9
    Conserved Domains (3) summary
    COG2453
    Location:207 → 300
    CDC14; Protein-tyrosine phosphatase [Signal transduction mechanisms]
    cd00127
    Location:158 → 295
    DSPc; Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Structurally similar to tyrosine-specific phosphatases but with a shallower active site cleft and a distinctive active site signature motif, HCxxGxxR. Characterized as VHR- or ...
    cd01446
    Location:10 → 136
    DSP_MapKP; N-terminal regulatory rhodanese domain of dual specificity phosphatases (DSP), such as Mapk Phosphatase. This domain is believed to determine substrate specificity by binding the substrate, such as ERK2, and activating the C-terminal catalytic domain by ...

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000012.12 Reference GRCh38.p14 Primary Assembly

    Range
    12473282..12562863 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_047429568.1 → XP_047285524.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  2. XM_047429570.1 → XP_047285526.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  3. XM_006719155.3 → XP_006719218.1  dual specificity protein phosphatase 16 isoform X1

    See identical proteins and their annotated locations for XP_006719218.1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3
    UniProtKB/TrEMBL
    Q32MA0, Q96N49
    Conserved Domains (3) summary
    COG2453
    Location:207 → 300
    CDC14; Protein-tyrosine phosphatase [Signal transduction mechanisms]
    cd00127
    Location:158 → 295
    DSPc; Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Structurally similar to tyrosine-specific phosphatases but with a shallower active site cleft and a distinctive active site signature motif, HCxxGxxR. Characterized as VHR- or ...
    cd01446
    Location:10 → 136
    DSP_MapKP; N-terminal regulatory rhodanese domain of dual specificity phosphatases (DSP), such as Mapk Phosphatase. This domain is believed to determine substrate specificity by binding the substrate, such as ERK2, and activating the C-terminal catalytic domain by ...

  4. XM_011520856.2 → XP_011519158.1  dual specificity protein phosphatase 16 isoform X1

    See identical proteins and their annotated locations for XP_011519158.1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3
    UniProtKB/TrEMBL
    Q32MA0, Q96N49
    Conserved Domains (3) summary
    COG2453
    Location:207 → 300
    CDC14; Protein-tyrosine phosphatase [Signal transduction mechanisms]
    cd00127
    Location:158 → 295
    DSPc; Dual specificity phosphatases (DSP); Ser/Thr and Tyr protein phosphatases. Structurally similar to tyrosine-specific phosphatases but with a shallower active site cleft and a distinctive active site signature motif, HCxxGxxR. Characterized as VHR- or ...
    cd01446
    Location:10 → 136
    DSP_MapKP; N-terminal regulatory rhodanese domain of dual specificity phosphatases (DSP), such as Mapk Phosphatase. This domain is believed to determine substrate specificity by binding the substrate, such as ERK2, and activating the C-terminal catalytic domain by ...

  5. XM_047429569.1 → XP_047285525.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  6. XM_047429571.1 → XP_047285527.1  dual specificity protein phosphatase 16 isoform X2

Reference GRCh38.p14 PATCHES

Genomic

  1. NW_011332696.1 Reference GRCh38.p14 PATCHES

    Range
    442285..531866 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054331700.1 → XP_054187675.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  2. XM_054331705.1 → XP_054187680.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  3. XM_054331702.1 → XP_054187677.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  4. XM_054331701.1 → XP_054187676.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  5. XM_054331703.1 → XP_054187678.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  6. XM_054331706.1 → XP_054187681.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  7. XM_054331704.1 → XP_054187679.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  8. XM_054331707.1 → XP_054187682.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  9. XM_054331708.1 → XP_054187683.1  dual specificity protein phosphatase 16 isoform X2

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060936.1 Alternate T2T-CHM13v2.0

    Range
    12342454..12456119 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054373306.1 → XP_054229281.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  2. XM_054373298.1 → XP_054229273.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  3. XM_054373303.1 → XP_054229278.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  4. XM_054373300.1 → XP_054229275.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  5. XM_054373299.1 → XP_054229274.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  6. XM_054373301.1 → XP_054229276.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  7. XM_054373304.1 → XP_054229279.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  8. XM_054373302.1 → XP_054229277.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  9. XM_054373305.1 → XP_054229280.1  dual specificity protein phosphatase 16 isoform X1

    UniProtKB/Swiss-Prot
    Q547C7, Q96QS2, Q9BY84, Q9C0G3

  10. XM_054373307.1 → XP_054229282.1  dual specificity protein phosphatase 16 isoform X2

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9BY84.1 GenPept UniProtKB/Swiss-Prot:Q9BY84

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