Cyb5b cytochrome b5 type B [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Cyb5bprovided by RGD
Official Full Name: cytochrome b5 type Bprovided by RGD
Primary source: RGD:621551
See related: EnsemblRapid:ENSRNOG00000011142 AllianceGenome:RGD:621551
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: omb5; Cyb5m
Summary: Enables enzyme activator activity. Predicted to be involved in nitric oxide biosynthetic process. Located in mitochondrial outer membrane. Orthologous to human CYB5B (cytochrome b5 type B). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Adrenal (RPKM 420.4), Kidney (RPKM 377.6) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 19q12

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	19	NC_086037.1 (51972611..52006477)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	19	NC_051354.1 (35062871..35096741)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	19	NC_005118.4 (39357814..39391680)

Chromosome 19 - NC_086037.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The presence of a novel reductive enzyme system of importance for lipid synthesis that is exclusively localized to the outer mitochondrial membrane and is composed of CYB5B, MOSC2, and a third unknown component (a CYB5B reductase).
Title: Amidoxime reductase system containing cytochrome b5 type B (CYB5B) and MOSC2 is of importance for lipid synthesis in adipocyte mitochondria.
comparison of X-ray crystal structures of human and rat CYB5B reveals a striking difference in packing involving the five-strand beta-sheet, which can be attributed to fully buried residue 21 in strand beta4
Title: Accommodating a nonconservative internal mutation by water-mediated hydrogen bonding between β-sheet strands: a comparison of human and rat type B (mitochondrial) cytochrome b5.
OMb, present abundantly in zona glomerulosa (zG), participates in aldosterone formation in zG of rat under angiotensin II-stimulation through regeneration of ascorbic acid.
Title: Possible participation of outer mitochondrial membrane cytonchrome B5 in steroidogenesis in zona glomerulosa of rat adrenal cortex.
Molecular dynamics simulation studies of this protein were made and some of its physical properties described.
Title: Molecular dynamics simulation studies of cytochrome b5 from outer mitochondrial and microsomal membrane.
outer mitochondrial membrane cytochrome b5 and not microsomal membrane cytochrome b5 functions as an activator for androgenesis in rat Leydig cells.
Title: Identification of outer mitochondrial membrane cytochrome b5 as a modulator for androgen synthesis in Leydig cells.
simulations provided qualitative microscopic explanations of many of the differences in physical properties between outer mitochondrial membrane CYB5 isoform and microsomal CYB5 and two mutants in terms of localized changes in structure and flexibility
Title: Influence of point mutations on the flexibility of cytochrome b5: molecular dynamics simulations of holoproteins.

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General gene information

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Markers

RH130021 (e-PCR)
- UniSTS:213309

RH140205 (e-PCR)
- UniSTS:222284

AI009319 (e-PCR)
- UniSTS:261711

RH137527 (e-PCR)
- UniSTS:219615

RH143182 (e-PCR)
- UniSTS:232429

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables enzyme activator activity	IDA Inferred from Direct Assay more info	PubMed
enables heme binding	IBA Inferred from Biological aspect of Ancestor more info
enables heme binding	IEA Inferred from Electronic Annotation more info
enables heme binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
contributes_to nitrite reductase (NO-forming) activity	ISO Inferred from Sequence Orthology more info
enables ubiquinol-cytochrome-c reductase activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in nitric oxide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in proton transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in intracellular membrane-bounded organelle	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in mitochondrial outer membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
part_of nitric-oxide synthase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: cytochrome b5 type B

Names: cytochrome b5 type B (outer mitochondrial membrane)

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_030586.2 → NP_085075.1 cytochrome b5 type B precursor

See identical proteins and their annotated locations for NP_085075.1

Status: PROVISIONAL

Source sequence(s)

BC072535

UniProtKB/Swiss-Prot

P04166, Q9QWG1

UniProtKB/TrEMBL

A0A8L2Q7E2, A0A8L2R080, A6IYZ3

Related

ENSRNOP00000093641.1, ENSRNOT00000099870.2

Conserved Domains (2) summary

pfam00173
Location:24 → 96

Cyt-b5; Cytochrome b5-like Heme/Steroid binding domain

cl00200
Location:109 → 139

MIP; Major intrinsic protein (MIP) superfamily. Members of the MIP superfamily function as membrane channels that selectively transport water, small neutral molecules, and ions out of and between cells. The channel proteins share a common fold: the N-terminal ...