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NOX5 NADPH oxidase 5 [ Homo sapiens (human) ]

Gene ID: 79400, updated on 25-Mar-2024

Summary

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Official Symbol
NOX5provided by HGNC
Official Full Name
NADPH oxidase 5provided by HGNC
Primary source
HGNC:HGNC:14874
See related
Ensembl:ENSG00000290203 MIM:606572; AllianceGenome:HGNC:14874
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Summary
Enables proton channel activity and superoxide-generating NAD(P)H oxidase activity. Involved in several processes, including endothelial cell proliferation; positive regulation of reactive oxygen species metabolic process; and superoxide anion generation. Located in endoplasmic reticulum. [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in spleen (RPKM 7.9), testis (RPKM 4.2) and 3 other tissues See more
Orthologs
all
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Genomic context

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Location:
15q23
Exon count:
16
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 15 NC_000015.10 (68930525..69062762)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 15 NC_060939.1 (66753043..66885315)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 15 NC_000015.9 (69222864..69355102)

Chromosome 15 - NC_000015.10Genomic Context describing neighboring genes Neighboring gene acidic nuclear phosphoprotein 32 family member A Neighboring gene H3K4me1 hESC enhancer GRCh37_chr15:69103956-69104456 Neighboring gene ANP32A intronic transcript 1 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 9651 Neighboring gene H3K27ac hESC enhancer GRCh37_chr15:69109604-69110186 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6590 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6591 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr15:69111351-69111932 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr15:69111933-69112514 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6593 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6594 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 6595 Neighboring gene OCT4-NANOG hESC enhancer GRCh37_chr15:69115796-69116596 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 9652 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 9653 Neighboring gene cytokine induced apoptosis inhibitor 1 pseudogene Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr15:69167471-69168078 Neighboring gene MPRA-validated peak2374 silencer Neighboring gene sperm equatorial segment protein 1 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr15:69308141-69308642 Neighboring gene ATP binding cassette subfamily G member 2 (Junior blood group) pseudogene Neighboring gene H3K27ac hESC enhancer GRCh37_chr15:69318396-69318896 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr15:69328421-69329620 Neighboring gene ReSE screen-validated silencer GRCh37_chr15:69341256-69341437 Neighboring gene NANOG-H3K4me1 hESC enhancer GRCh37_chr15:69352321-69352820 Neighboring gene uncharacterized LOC124903515 Neighboring gene OCT4-NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr15:69378684-69379278 Neighboring gene H3K27ac hESC enhancer GRCh37_chr15:69382031-69382532 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr15:69385773-69386273 Neighboring gene Ewing sarcoma associated transcript 1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Structure, regulation, and physiological functions of NADPH oxidase 5 (NOX5). García JG, et al. J Physiol Biochem, 2023 May. PMID 36905456, Free PMC Article
  2. Ca(2+) -binding-region-dependent cell surface localization of NADPH oxidase Nox5. Miyano K, et al. FEBS Lett, 2023 Mar. PMID 36653838
  3. Impact of calcium and reactive oxygen species on human sperm function: Role of NOX5. Keshtgar S, et al. Andrologia, 2022 Sep. PMID 35679508
  4. Independent of Renox, NOX5 Promotes Renal Inflammation and Fibrosis in Diabetes by Activating ROS-Sensitive Pathways. Jha JC, et al. Diabetes, 2022 Jun 1. PMID 35275988
  5. NOX5 mediates the crosstalk between tumor cells and cancer-associated fibroblasts via regulating cytokine network. Chen J, et al. Clin Transl Med, 2021 Aug. PMID 34459125, Free PMC Article

See all (104) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Structure, regulation, and physiological functions of NADPH oxidase 5 (NOX5).
    Title: Structure, regulation, and physiological functions of NADPH oxidase 5 (NOX5).
  2. Ca[2+] -binding-region-dependent cell surface localization of NADPH oxidase Nox5.
    Title: Ca(2+) -binding-region-dependent cell surface localization of NADPH oxidase Nox5.
  3. NOX2 and NOX5 are increased in cardiac microvascular endothelium of deceased COVID-19 patients.
    Title: NOX2 and NOX5 are increased in cardiac microvascular endothelium of deceased COVID-19 patients.
  4. Impact of calcium and reactive oxygen species on human sperm function: Role of NOX5.
    Title: Impact of calcium and reactive oxygen species on human sperm function: Role of NOX5.
  5. Nicotine promotes vascular calcification via intracellular Ca2+-mediated, Nox5-induced oxidative stress, and extracellular vesicle release in vascular smooth muscle cells.
    Title: Nicotine promotes vascular calcification via intracellular Ca2+-mediated, Nox5-induced oxidative stress, and extracellular vesicle release in vascular smooth muscle cells.
  6. Independent of Renox, NOX5 Promotes Renal Inflammation and Fibrosis in Diabetes by Activating ROS-Sensitive Pathways.
    Title: Independent of Renox, NOX5 Promotes Renal Inflammation and Fibrosis in Diabetes by Activating ROS-Sensitive Pathways.
  7. NOX5 mediates the crosstalk between tumor cells and cancer-associated fibroblasts via regulating cytokine network.
    Title: NOX5 mediates the crosstalk between tumor cells and cancer-associated fibroblasts via regulating cytokine network.
  8. NADPH oxidase 5 has a crucial role in cellular motility of colon cancer cells.
    Title: NADPH oxidase 5 has a crucial role in cellular motility of colon cancer cells.
  9. Membranous NOX5-derived ROS oxidizes and activates local Src to promote malignancy of tumor cells.
    Title: Membranous NOX5-derived ROS oxidizes and activates local Src to promote malignancy of tumor cells.
  10. Implications of NADPH oxidase 5 in vascular diseases.
    Title: Implications of NADPH oxidase 5 in vascular diseases.
Submit: New GeneRIF Correction See all GeneRIFs (86)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide association study evaluating lipoprotein-associated phospholipase A2 mass and activity at baseline and after rosuvastatin therapy.
EBI GWAS Catalog
Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env HIV-1 gp120 binding to CXCR4 induces NADPH oxidase-mediated production of superoxide radicals in neurons, which is involved in the activation of neutral sphingomyelinase PubMed
Envelope surface glycoprotein gp160, precursor env HIV-1 gp160 inhibits the cellular immune response and enhances oxidative stress mediated by NADPH oxidase PubMed
Tat tat PDGF-BB inhibits NADPH oxidase-mediated reactive oxygen species production induced by HIV-1 Tat and morphine treatment in neuroblastoma cells PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Readthrough SPESP1-NOX5

Readthrough gene: SPESP1-NOX5, Included gene: SPESP1

Homology

Clone Names

  • MGC149776, MGC149777

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables NADP binding NAS
Non-traceable Author Statement
more info
 PubMed 
enables calcium ion binding IEA
Inferred from Electronic Annotation
more info
  
enables flavin adenine dinucleotide binding NAS
Non-traceable Author Statement
more info
 PubMed 
enables heme binding NAS
Non-traceable Author Statement
more info
 PubMed 
enables proton channel activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables superoxide-generating NAD(P)H oxidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables superoxide-generating NAD(P)H oxidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables superoxide-generating NADPH oxidase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in angiogenesis IEA
Inferred from Electronic Annotation
more info
  
involved_in apoptotic process NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in cytoskeleton-dependent cytokinesis NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in defense response IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in endothelial cell proliferation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of cytokine production NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in proton transmembrane transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of fusion of sperm to egg plasma membrane NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in superoxide anion generation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in superoxide anion generation IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of NADPH oxidase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum membrane TAS
Traceable Author Statement
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
NADPH oxidase 5
Names
NADPH oxidase, EF-hand calcium binding domain 5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_030464.2 RefSeqGene

    Range
    89172..137239
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001184779.2 → NP_001171708.1  NADPH oxidase 5 isoform 2

    See identical proteins and their annotated locations for NP_001171708.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2, alternately referred to as NOX5 alpha and v1) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.
    Source sequence(s)
    AC027088, AF353088, AI091544, DA868876, DQ413001
    Consensus CDS
    CCDS53954.1
    UniProtKB/Swiss-Prot
    Q96PH1
    UniProtKB/TrEMBL
    A3QRJ0
    Conserved Domains (4) summary
    cd06186
    Location:420 → 737
    NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...
    cd00051
    Location:97 → 164
    EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...
    pfam01794
    Location:268 → 374
    Ferric_reduct; Ferric reductase like transmembrane component
    pfam13499
    Location:98 → 162
    EF-hand_7; EF-hand domain pair

  2. NM_024505.4 → NP_078781.3  NADPH oxidase 5 isoform 1

    See identical proteins and their annotated locations for NP_078781.3

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1, alternately referred to as NOX5 gamma and v3) encodes the longest isoform (1).
    Source sequence(s)
    AC027088, AF353089, AI091544, DA868876, DQ413001
    Consensus CDS
    CCDS32276.2
    UniProtKB/Swiss-Prot
    B2RBJ4, Q08AN2, Q08AN3, Q8TEQ1, Q8TER4, Q96PH1, Q96PH2, Q96PJ8, Q96PJ9, Q9H6E0, Q9HAM8
    Conserved Domains (4) summary
    cd06186
    Location:448 → 765
    NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...
    cd00051
    Location:97 → 192
    EFh; EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to ...
    pfam01794
    Location:296 → 402
    Ferric_reduct; Ferric reductase like transmembrane component
    pfam13499
    Location:98 → 190
    EF-hand_7; EF-hand domain pair

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000015.10 Reference GRCh38.p14 Primary Assembly

    Range
    68930525..69062762
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060939.1 Alternate T2T-CHM13v2.0

    Range
    66753043..66885315
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q96PH1.1 GenPept UniProtKB/Swiss-Prot:Q96PH1

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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