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Epn2 epsin 2 [ Rattus norvegicus (Norway rat) ]

Gene ID: 60443, updated on 10-Oct-2024

Summary

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Official Symbol
Epn2provided by RGD
Official Full Name
epsin 2provided by RGD
Primary source
RGD:619773
See related
EnsemblRapid:ENSRNOG00000060550 AllianceGenome:RGD:619773
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary
The protein encoded by this gene is a member of the epsin protein family. Epsin proteins are endocytic adaptors that function in the formation of clatherin-coated vesicles. Epsins contain a highly conserved N-terminal homology domain that binds phosphatidylinositol 4,5-bisphosphate in the plasma membrane, two or three ubiquitin interacting motifs, two clathrin-binding motifs, a cluster of aspartate-proline-tryptophan/phenylalanine repeats, and two or three asparagine-proline-phenylalanine tripeptide repeats at the C-terminus. In mouse, simultaneous knockout of this gene and its paralog results in embryonic arrest due to disruption of Notch signaling, suggesting a role as a specialized endocytic adaptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
Expression
Biased expression in Brain (RPKM 238.6), Lung (RPKM 228.9) and 9 other tissues See more
Orthologs
human mouse all
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Genomic context

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See Epn2 in Genome Data Viewer
Location:
10q22
Exon count:
16
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 10 NC_086028.1 (46697238..46759128, complement)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 10 NC_051345.1 (46197785..46259673, complement)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 10 NC_005109.4 (47795496..47857373, complement)

Chromosome 10 - NC_086028.1Genomic Context describing neighboring genes Neighboring gene microfibril associated protein 4 Neighboring gene mitogen-activated protein kinase 7 Neighboring gene B9 domain containing 1 Neighboring gene transfer RNA valine (anticodon AAC) 10 Neighboring gene uncharacterized LOC134480802 Neighboring gene GRB2-related adaptor protein

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. The epsin protein family: coordinators of endocytosis and signaling. Sen A, et al. Biomol Concepts, 2012 Apr. PMID 22942912, Free PMC Article
  2. A mammalian mirtron miR-1224 promotes tube-formation of human primary endothelial cells by targeting anti-angiogenic factor epsin2. Sakai E, et al. Sci Rep, 2017 Jul 17. PMID 28717225, Free PMC Article
  3. A single motif responsible for ubiquitin recognition and monoubiquitination in endocytic proteins. Polo S, et al. Nature, 2002 Mar 28. PMID 11919637
  4. A role for epsin N-terminal homology/AP180 N-terminal homology (ENTH/ANTH) domains in tubulin binding. Hussain NK, et al. J Biol Chem, 2003 Aug 1. PMID 12750376
  5. The epsins define a family of proteins that interact with components of the clathrin coat and contain a new protein module. Rosenthal JA, et al. J Biol Chem, 1999 Nov 26. PMID 10567358

See all (10) citations in PubMed

GeneRIFs: Gene References Into Functions

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC105438

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables clathrin binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables lipid binding IEA
Inferred from Electronic Annotation
more info
  
enables phospholipid binding IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within Notch signaling pathway ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within embryonic organ development ISO
Inferred from Sequence Orthology
more info
  
involved_in endocytosis IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within in utero embryonic development ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of sprouting angiogenesis IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of sprouting angiogenesis ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of vascular endothelial growth factor receptor signaling pathway IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of vascular endothelial growth factor receptor signaling pathway ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of Notch signaling pathway IEA
Inferred from Electronic Annotation
more info
  
involved_in positive regulation of Notch signaling pathway ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of endocytosis IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of clathrin coat of endocytic vesicle IDA
Inferred from Direct Assay
more info
 PubMed 
part_of clathrin vesicle coat IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in endosome IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in intracellular membrane-bounded organelle IEA
Inferred from Electronic Annotation
more info
  
located_in intracellular membrane-bounded organelle ISO
Inferred from Sequence Orthology
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in postsynaptic endocytic zone IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in postsynaptic endocytic zone IMP
Inferred from Mutant Phenotype
more info
 PubMed 

General protein information

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Preferred Names
epsin-2
Names
EH domain binding protein epsin 2
EPS-15-interacting protein 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001033914.2 → NP_001029086.1  epsin-2 isoform b

    See identical proteins and their annotated locations for NP_001029086.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an alternate exon in the 5' UTR compared to variant 3. Variants 2 and 3 encode isoform b.
    Source sequence(s)
    BC094524, CB583573, JAXUCZ010000010
    UniProtKB/TrEMBL
    A6HF84, Q505I9
    Conserved Domains (1) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

  2. NM_001305628.1 → NP_001292557.1  epsin-2 isoform b

    See identical proteins and their annotated locations for NP_001292557.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) represents the longest transcript and encodes the longer isoform (b). Variants 2 and 3 encode the same isoform (b).
    Source sequence(s)
    BC094524, CB583573, JAXUCZ010000010
    UniProtKB/TrEMBL
    A6HF84, Q505I9
    Related
    ENSRNOP00000074461.1, ENSRNOT00000085703.3
    Conserved Domains (1) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

  3. NM_021852.3 → NP_068624.2  epsin-2 isoform a

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) differs in its 5' UTR and lacks an alternate in-frame exon in the 5' coding region compared to variant 3. It encodes isoform a, which is shorter than isoform b.
    Source sequence(s)
    BC094524, JAXUCZ010000010
    UniProtKB/TrEMBL
    A6HF84
    Conserved Domains (2) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
    cl00427
    Location:335 → 399
    TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086028.1 Reference GRCr8

    Range
    46697238..46759128 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_039086747.2 → XP_038942675.1  epsin-2 isoform X3

    UniProtKB/TrEMBL
    A6HF84, F1LQ45
    Related
    ENSRNOP00000106806.1, ENSRNOT00000155858.1
    Conserved Domains (2) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
    cl00427
    Location:347 → 411
    TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...

  2. XM_039086748.2 → XP_038942676.1  epsin-2 isoform X4

    UniProtKB/TrEMBL
    A6HF84
    Conserved Domains (2) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
    cl00427
    Location:335 → 399
    TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...

  3. XM_006246521.5 → XP_006246583.1  epsin-2 isoform X1

    UniProtKB/TrEMBL
    A6HF84
    Conserved Domains (2) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
    cl00427
    Location:404 → 468
    TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...

  4. XM_039086749.2 → XP_038942677.1  epsin-2 isoform X4

    UniProtKB/TrEMBL
    A6HF84
    Conserved Domains (2) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
    cl00427
    Location:335 → 399
    TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...

  5. XM_063269770.1 → XP_063125840.1  epsin-2 isoform X3

    UniProtKB/TrEMBL
    F1LQ45

  6. XM_017597507.3 → XP_017452996.1  epsin-2 isoform X1

    UniProtKB/TrEMBL
    A6HF84
    Related
    ENSRNOP00000073697.3, ENSRNOT00000091963.3
    Conserved Domains (2) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
    cl00427
    Location:404 → 468
    TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...

  7. XM_063269779.1 → XP_063125849.1  epsin-2 isoform X4

  8. XM_017597506.3 → XP_017452995.1  epsin-2 isoform X1

    UniProtKB/TrEMBL
    A6HF84
    Conserved Domains (2) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
    cl00427
    Location:404 → 468
    TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...

  9. XM_063269768.1 → XP_063125838.1  epsin-2 isoform X3

    UniProtKB/TrEMBL
    F1LQ45

  10. XM_063269767.1 → XP_063125837.1  epsin-2 isoform X2

    UniProtKB/TrEMBL
    Q505I9

  11. XM_017597511.2 → XP_017453000.1  epsin-2 isoform X1

    UniProtKB/TrEMBL
    A6HF84
    Conserved Domains (2) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
    cl00427
    Location:404 → 468
    TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...

  12. XM_063269775.1 → XP_063125845.1  epsin-2 isoform X3

    UniProtKB/TrEMBL
    F1LQ45

  13. XM_039086746.2 → XP_038942674.1  epsin-2 isoform X2

    UniProtKB/TrEMBL
    A6HF84, Q505I9
    Conserved Domains (1) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family

  14. XM_063269781.1 → XP_063125851.1  epsin-2 isoform X4

  15. XM_039086741.2 → XP_038942669.1  epsin-2 isoform X1

    UniProtKB/TrEMBL
    A6HF84
    Conserved Domains (2) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
    cl00427
    Location:404 → 468
    TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...

  16. XM_039086743.2 → XP_038942671.1  epsin-2 isoform X1

    UniProtKB/TrEMBL
    A6HF84
    Conserved Domains (2) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
    cl00427
    Location:404 → 468
    TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...

  17. XM_017597508.3 → XP_017452997.1  epsin-2 isoform X1

    UniProtKB/TrEMBL
    A6HF84
    Conserved Domains (2) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
    cl00427
    Location:404 → 468
    TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...

  18. XM_063269776.1 → XP_063125846.1  epsin-2 isoform X3

    UniProtKB/TrEMBL
    F1LQ45

  19. XM_063269772.1 → XP_063125842.1  epsin-2 isoform X3

    UniProtKB/TrEMBL
    F1LQ45

  20. XM_063269782.1 → XP_063125852.1  epsin-2 isoform X4

  21. XM_063269780.1 → XP_063125850.1  epsin-2 isoform X4

  22. XM_039086751.2 → XP_038942679.1  epsin-2 isoform X5

  23. XM_039086745.2 → XP_038942673.1  epsin-2 isoform X1

    UniProtKB/TrEMBL
    A6HF84
    Conserved Domains (2) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
    cl00427
    Location:404 → 468
    TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...

  24. XM_039086742.2 → XP_038942670.1  epsin-2 isoform X1

    UniProtKB/TrEMBL
    A6HF84
    Conserved Domains (2) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
    cl00427
    Location:404 → 468
    TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...

  25. XM_063269765.1 → XP_063125835.1  epsin-2 isoform X1

  26. XM_063269769.1 → XP_063125839.1  epsin-2 isoform X3

    UniProtKB/TrEMBL
    F1LQ45

  27. XM_039086740.2 → XP_038942668.1  epsin-2 isoform X1

    UniProtKB/TrEMBL
    A6HF84
    Conserved Domains (2) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
    cl00427
    Location:404 → 468
    TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...

  28. XM_063269766.1 → XP_063125836.1  epsin-2 isoform X1

  29. XM_063269764.1 → XP_063125834.1  epsin-2 isoform X1

  30. XM_039086744.2 → XP_038942672.1  epsin-2 isoform X1

    UniProtKB/TrEMBL
    A6HF84
    Conserved Domains (2) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
    cl00427
    Location:404 → 468
    TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...

  31. XM_017597510.3 → XP_017452999.1  epsin-2 isoform X1

    UniProtKB/TrEMBL
    A6HF84
    Conserved Domains (2) summary
    cd16990
    Location:19 → 141
    ENTH_Epsin; Epsin N-Terminal Homology (ENTH) domain of Epsin family
    cl00427
    Location:404 → 468
    TM_PBP2; Transmembrane subunit (TM) found in Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporters which generally bind type 2 PBPs. These types of transporters consist of a PBP, two TMs, and two cytoplasmic ABC ATPase subunits, and ...

  32. XM_063269778.1 → XP_063125848.1  epsin-2 isoform X3

    UniProtKB/TrEMBL
    F1LQ45

  33. XM_063269774.1 → XP_063125844.1  epsin-2 isoform X3

    UniProtKB/TrEMBL
    F1LQ45

  34. XM_063269777.1 → XP_063125847.1  epsin-2 isoform X3

    UniProtKB/TrEMBL
    F1LQ45

  35. XM_063269773.1 → XP_063125843.1  epsin-2 isoform X3

    UniProtKB/TrEMBL
    F1LQ45

  36. XM_063269783.1 → XP_063125853.1  epsin-2 isoform X4

  37. XM_063269771.1 → XP_063125841.1  epsin-2 isoform X3

    UniProtKB/TrEMBL
    F1LQ45
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9Z1Z3.1 GenPept UniProtKB/Swiss-Prot:Q9Z1Z3

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