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ANO2 anoctamin 2 [ Homo sapiens (human) ]

Gene ID: 57101, updated on 13-Apr-2024

Summary

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Official Symbol
ANO2provided by HGNC
Official Full Name
anoctamin 2provided by HGNC
Primary source
HGNC:HGNC:1183
See related
Ensembl:ENSG00000047617 MIM:610109; AllianceGenome:HGNC:1183
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
C12orf3; TMEM16B
Summary
ANO2 belongs to a family of calcium-activated chloride channels (CaCCs) (reviewed by Hartzell et al., 2009 [PubMed 19015192]).[supplied by OMIM, Jan 2011]
Expression
Low expression observed in reference dataset See more
Orthologs
mouse all
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Genomic context

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Location:
12p13.31
Exon count:
28
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 12 NC_000012.12 (5562655..5946232, complement)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 12 NC_060936.1 (5569523..5953779, complement)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 12 NC_000012.11 (5671821..6055398, complement)

Chromosome 12 - NC_000012.12Genomic Context describing neighboring genes Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr12:5542091-5542616 Neighboring gene uncharacterized LOC124902865 Neighboring gene neurotrophin 3 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr12:5618629-5619828 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr12:5626075-5626631 Neighboring gene BRD4-independent group 4 enhancer GRCh37_chr12:5629720-5630919 Neighboring gene OCT4 hESC enhancer GRCh37_chr12:5681388-5681889 Neighboring gene dynein light chain roadblock-type 1 pseudogene Neighboring gene meiotic recombination hotspot F Neighboring gene MPRA-validated peak1541 silencer Neighboring gene meiotic recombination hotspot 12D Neighboring gene P300/CBP strongly-dependent group 1 enhancer GRCh37_chr12:5916007-5917206 Neighboring gene H3K27ac hESC enhancer GRCh37_chr12:5921895-5922394 Neighboring gene NANOG hESC enhancer GRCh37_chr12:5927975-5928488 Neighboring gene NANOG-H3K4me1 hESC enhancer GRCh37_chr12:5936424-5937202 Neighboring gene uncharacterized LOC105369621 Neighboring gene MPRA-validated peak1543 silencer Neighboring gene H3K4me1 hESC enhancer GRCh37_chr12:6017201-6017701 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr12:6030281-6030782 Neighboring gene P300/CBP strongly-dependent group 1 enhancer GRCh37_chr12:6040249-6041448 Neighboring gene ReSE screen-validated silencer GRCh37_chr12:6075167-6075353 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr12:6076959-6077460 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr12:6078733-6079346 Neighboring gene Sharpr-MPRA regulatory region 15487 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr12:6127715-6128214 Neighboring gene von Willebrand factor Neighboring gene H3K4me1 hESC enhancer GRCh37_chr12:6167267-6167774 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr12:6167775-6168282 Neighboring gene Sharpr-MPRA regulatory region 3083 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 4156 Neighboring gene small nucleolar RNA, H/ACA box 120 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr12:6192008-6192754 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr12:6192755-6193499 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr12:6202408-6203004 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr12:6203005-6203600 Neighboring gene RNA, 7SL, cytoplasmic 69, pseudogene

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Permeation Mechanisms in the TMEM16B Calcium-Activated Chloride Channels. Pifferi S. PLoS One, 2017. PMID 28046119, Free PMC Article
  2. Anoctamin 2 identified as an autoimmune target in multiple sclerosis. Ayoglu B, et al. Proc Natl Acad Sci U S A, 2016 Feb 23. PMID 26862169, Free PMC Article
  3. Assessment of the olfactory function in Italian patients with type 3 von Willebrand disease caused by a homozygous 253 Kb deletion involving VWF and TMEM16B/ANO2. Cenedese V, et al. PLoS One, 2015. PMID 25635880, Free PMC Article
  4. Anoctamin 2-chloride channels reduce simple spike activity and mediate inhibition at elevated calcium concentration in cerebellar Purkinje cells. Auer F, et al. PLoS One, 2021. PMID 33651839, Free PMC Article
  5. Molecular mimicry between Anoctamin 2 and Epstein-Barr virus nuclear antigen 1 associates with multiple sclerosis risk. Tengvall K, et al. Proc Natl Acad Sci U S A, 2019 Aug 20. PMID 31375628, Free PMC Article

See all (29) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Anoctamin 2-chloride channels reduce simple spike activity and mediate inhibition at elevated calcium concentration in cerebellar Purkinje cells.
    Title: Anoctamin 2-chloride channels reduce simple spike activity and mediate inhibition at elevated calcium concentration in cerebellar Purkinje cells.
  2. a hypothesis where immune reactivity toward EBNA1 through molecular mimicry with ANO2 contributes to the etiopathogenesis of Multiple sclerosis.
    Title: Molecular mimicry between Anoctamin 2 and Epstein-Barr virus nuclear antigen 1 associates with multiple sclerosis risk.
  3. residues facing the putative channel pore are responsible both for controlling the ion selectivity and the gating of the channel, providing an initial understanding of molecular mechanism of ion permeation in TMEM16B
    Title: Permeation Mechanisms in the TMEM16B Calcium-Activated Chloride Channels.
  4. data revealed prominently increased autoantibody reactivity against the chloride-channel protein anoctamin 2 (ANO2) in multiple sclerosis cases compared with controls
    Title: Anoctamin 2 identified as an autoimmune target in multiple sclerosis.
  5. Olfactory function impairment in Italian patients with type 3 von Willebrand disease with partial deletion of TMEM16B.
    Title: Assessment of the olfactory function in Italian patients with type 3 von Willebrand disease caused by a homozygous 253 Kb deletion involving VWF and TMEM16B/ANO2.
  6. results reveal multidimensional regulation of TMEM16A/16B by preassociated apoCaM and introduce ChIMP as a versatile tool to probe the macromolecular complex and function of Ca(2+)-activated chloride channels
    Title: Preassociated apocalmodulin mediates Ca2+-dependent sensitization of activation and inactivation of TMEM16A/16B Ca2+-gated Cl- channels.
  7. ANO2 is present in human myometrium.
    Title: Calcium-activated chloride channels anoctamin 1 and 2 promote murine uterine smooth muscle contractility.
  8. The third intracellular loop of TMEM16B is the site involved in calcium ion sensitivity, whereas the C-terminal part affects the rate of transition between the open and the closed state of the channel.
    Title: TMEM16A-TMEM16B chimaeras to investigate the structure-function relationship of calcium-activated chloride channels.
  9. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
    Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
  10. Ano2 likely amplifies the odor-induced generator potential in olfactory sensory neuron cilia by sensing elevated calcium levels, permitting the outward flow of chloride ions from the cell.
    Title: ANO2 is the cilial calcium-activated chloride channel that may mediate olfactory amplification.
Submit: New GeneRIF Correction See all GeneRIFs (14)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide association study of panic disorder in the Japanese population.
EBI GWAS Catalog
Loci associated with N-glycosylation of human immunoglobulin G show pleiotropy with autoimmune diseases and haematological cancers.
EBI GWAS Catalog
Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • DKFZp434P102

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables intracellularly calcium-gated chloride channel activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables intracellularly calcium-gated chloride channel activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables intracellularly calcium-gated chloride channel activity TAS
Traceable Author Statement
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein dimerization activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in chloride transmembrane transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in monoatomic ion transmembrane transport TAS
Traceable Author Statement
more info
  
Component Evidence Code Pubs
part_of chloride channel complex IEA
Inferred from Electronic Annotation
more info
  
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
  
located_in plasma membrane ISS
Inferred from Sequence or Structural Similarity
more info
  
located_in plasma membrane TAS
Traceable Author Statement
more info
  

General protein information

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Preferred Names
anoctamin-2
Names
anoctamin 2, calcium activated chloride channel
transmembrane protein 16B (eight membrane-spanning domains)

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001278596.3 → NP_001265525.1  anoctamin-2 isoform 1

    See identical proteins and their annotated locations for NP_001265525.1

    Status: VALIDATED

    Source sequence(s)
    AB593159, AC006431, AK024010, BE645295, FJ384095
    Consensus CDS
    CCDS91643.1
    UniProtKB/Swiss-Prot
    C4N787, Q9H847, Q9NQ90
    Related
    ENSP00000498903.1, ENST00000650848.1
    Conserved Domains (2) summary
    pfam04547
    Location:352 → 943
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:91 → 349
    Anoct_dimer; Dimerization domain of Ca+-activated chloride-channel, anoctamin

  2. NM_001278597.3 → NP_001265526.1  anoctamin-2 isoform 2

    See identical proteins and their annotated locations for NP_001265526.1

    Status: VALIDATED

    Source sequence(s)
    AC006431, AC006560, AC137627
    Consensus CDS
    CCDS44807.2
    UniProtKB/Swiss-Prot
    Q9NQ90
    Related
    ENSP00000348453.5, ENST00000356134.9
    Conserved Domains (2) summary
    pfam04547
    Location:348 → 939
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:87 → 345
    Anoct_dimer; Dimerization domain of Ca+-activated chloride-channel, anoctamin

  3. NM_001364791.2 → NP_001351720.1  anoctamin-2 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AC006431, AC006560, AC137627
    Consensus CDS
    CCDS91642.1
    UniProtKB/TrEMBL
    A0A804HIY3
    Related
    ENSP00000507275.1, ENST00000682330.1
    Conserved Domains (3) summary
    COG3428
    Location:487 → 605
    YdbT; Uncharacterized membrane protein YdbT, contains bPH2 (bacterial pleckstrin homology) domain [Function unknown]
    pfam04547
    Location:351 → 937
    Anoctamin; Calcium-activated chloride channel
    pfam16178
    Location:165 → 348
    Anoct_dimer; Dimerisation domain of Ca+-activated chloride-channel, anoctamin

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000012.12 Reference GRCh38.p14 Primary Assembly

    Range
    5562655..5946232 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060936.1 Alternate T2T-CHM13v2.0

    Range
    5569523..5953779 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_020373.2: Suppressed sequence

    Description
    NM_020373.2: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9NQ90.2 GenPept UniProtKB/Swiss-Prot:Q9NQ90

Gene LinkOut

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