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Akap8 A kinase anchor protein 8 [ Mus musculus (house mouse) ]

Gene ID: 56399, updated on 2-Nov-2024

Summary

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Official Symbol
Akap8provided by MGI
Official Full Name
A kinase anchor protein 8provided by MGI
Primary source
MGI:MGI:1928488
See related
Ensembl:ENSMUSG00000024045 AllianceGenome:MGI:1928488
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
AKAP-8; AKAP95; 1200016A02Rik
Summary
This gene encodes a member of the A-kinase anchoring protein (AKAP) family. These proteins are characterized by their ability to bind to the R subunit of protein kinase A (PKA) and anchor the protein at different subcellular locations. This protein has been shown to regulate apoptosis and to be involved in palatogenesis. Knockdown of this gene has been associated with altered histone modifications and reduced expression of developmental genes in mouse embryonic stem cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
Expression
Ubiquitous expression in CNS E11.5 (RPKM 24.6), CNS E14 (RPKM 20.6) and 28 other tissues See more
Orthologs
human all
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Genomic context

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See Akap8 in Genome Data Viewer
Location:
17 B1; 17 17.48 cM
Exon count:
16
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 17 NC_000083.7 (32522646..32540212, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 17 NC_000083.6 (32303672..32321238, complement)

Chromosome 17 - NC_000083.7Genomic Context describing neighboring genes Neighboring gene epoxide hydrolase 3 Neighboring gene bromodomain containing 4 Neighboring gene predicted gene 4432 Neighboring gene STARR-positive B cell enhancer ABC_E11795 Neighboring gene STARR-positive B cell enhancer ABC_E5590 Neighboring gene STARR-positive B cell enhancer ABC_E955 Neighboring gene A kinase anchor protein 8-like Neighboring gene STARR-positive B cell enhancer ABC_E1502 Neighboring gene STARR-positive B cell enhancer mm9_chr17:32506256-32506557 Neighboring gene STARR-positive B cell enhancer mm9_chr17:32508595-32508896 Neighboring gene widely-interspaced zinc finger motifs Neighboring gene STARR-positive B cell enhancer ABC_E4205 Neighboring gene predicted gene, 53995

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Biophysical properties of AKAP95 protein condensates regulate splicing and tumorigenesis. Li W, et al. Nat Cell Biol, 2020 Aug. PMID 32719551, Free PMC Article
  2. DNA methylation, histone modifications and behaviour of AKAP95 during mouse oocyte growth and upon nuclear transfer of foreign chromatin into fully grown prophase oocytes. Hoffmann S, et al. Folia Biol (Krakow), 2012. PMID 23342911
  3. A-kinase-anchoring protein 95 functions as a potential carrier for the nuclear translocation of active caspase 3 through an enzyme-substrate-like association. Kamada S, et al. Mol Cell Biol, 2005 Nov. PMID 16227597, Free PMC Article
  4. Cloning and characterization of AKAP 95, a nuclear protein that associates with the regulatory subunit of type II cAMP-dependent protein kinase. Coghlan VM, et al. J Biol Chem, 1994 Mar 11. PMID 8125992
  5. Suppression of LPS-induced TNF-alpha production in macrophages by cAMP is mediated by PKA-AKAP95-p105. Wall EA, et al. Sci Signal, 2009 Jun 16. PMID 19531803, Free PMC Article

See all (50) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Biophysical properties of AKAP95 protein condensates regulate splicing and tumorigenesis.
    Title: Biophysical properties of AKAP95 protein condensates regulate splicing and tumorigenesis.
  2. This study showed an increase in the level of global DNA methylation starting in oocytes with diameters around 60 microm, increases in histone h4, and decreases in AKAP95.
    Title: DNA methylation, histone modifications and behaviour of AKAP95 during mouse oocyte growth and upon nuclear transfer of foreign chromatin into fully grown prophase oocytes.
  3. a specific role for A kinase-anchoring protein 95 in suppressing the expression of the gene encoding tumor necrosis factor-alpha, which involved phosphorylation of p105 (also known as Nfkb1) by PKA
    Title: Suppression of LPS-induced TNF-alpha production in macrophages by cAMP is mediated by PKA-AKAP95-p105.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (3) 
  • Gene trapped (1)  1 citation
  • Targeted (1) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables DNA binding ISO
Inferred from Sequence Orthology
more info
  
enables NF-kappaB binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables chromatin binding ISO
Inferred from Sequence Orthology
more info
  
enables double-stranded DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables double-stranded DNA binding ISO
Inferred from Sequence Orthology
more info
  
enables histone deacetylase binding IEA
Inferred from Electronic Annotation
more info
  
enables histone deacetylase binding ISO
Inferred from Sequence Orthology
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein kinase A regulatory subunit binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein kinase A regulatory subunit binding IEA
Inferred from Electronic Annotation
more info
  
enables protein kinase A regulatory subunit binding ISO
Inferred from Sequence Orthology
more info
  
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in cell cycle G2/M phase transition IEA
Inferred from Electronic Annotation
more info
  
involved_in cell cycle G2/M phase transition ISO
Inferred from Sequence Orthology
more info
  
involved_in cellular response to lipopolysaccharide IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cellular response to prostaglandin E stimulus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in innate immune response IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within mitotic chromosome condensation IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within mitotic chromosome condensation ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of tumor necrosis factor production IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in chromatin ISO
Inferred from Sequence Orthology
more info
  
located_in condensed chromosome IDA
Inferred from Direct Assay
more info
 PubMed 
located_in condensed chromosome ISO
Inferred from Sequence Orthology
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in female pronucleus IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nuclear matrix IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nuclear matrix IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nuclear matrix ISO
Inferred from Sequence Orthology
more info
  
located_in nucleolus IEA
Inferred from Electronic Annotation
more info
  
located_in nucleolus ISO
Inferred from Sequence Orthology
more info
  
located_in nucleoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
A-kinase anchor protein 8
Names
A kinase (PRKA) anchor protein 8
A-kinase anchor protein 95 kDa

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001357760.1 → NP_001344689.1  A-kinase anchor protein 8 isoform 2

    Status: REVIEWED

    Source sequence(s)
    BC060170, CT033751
    Conserved Domains (1) summary
    pfam04988
    Location:273 → 433
    AKAP95; A-kinase anchoring protein 95 (AKAP95)

  2. NM_019774.5 → NP_062748.2  A-kinase anchor protein 8 isoform 1

    See identical proteins and their annotated locations for NP_062748.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the shortest transcript and encodes the protein.
    Source sequence(s)
    AK004801, BC060170, BC060209
    Consensus CDS
    CCDS37555.1
    UniProtKB/Swiss-Prot
    Q9DBR0, Q9R0L8
    UniProtKB/TrEMBL
    Q059U9
    Related
    ENSMUSP00000002699.6, ENSMUST00000002699.7
    Conserved Domains (1) summary
    pfam04988
    Location:387 → 547
    AKAP95; A-kinase anchoring protein 95 (AKAP95)

RNA

  1. NR_131218.1 RNA Sequence

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses an alternate splice site in an internal exon compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    BC060170, BC060209
    Related
    ENSMUST00000237381.2

  2. NR_131219.1 RNA Sequence

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) lacks two alternate internal exons and uses an alternate splice site in an internal exon compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    BC054433, BC060170, BC060209

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000083.7 Reference GRCm39 C57BL/6J

    Range
    32522646..32540212 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006524665.5 → XP_006524728.1  A-kinase anchor protein 8 isoform X2

    See identical proteins and their annotated locations for XP_006524728.1

    Conserved Domains (1) summary
    pfam04988
    Location:273 → 433
    AKAP95; A-kinase anchoring protein 95 (AKAP95)

  2. XM_006524666.5 → XP_006524729.1  A-kinase anchor protein 8 isoform X2

    See identical proteins and their annotated locations for XP_006524729.1

    Conserved Domains (1) summary
    pfam04988
    Location:273 → 433
    AKAP95; A-kinase anchoring protein 95 (AKAP95)

  3. XM_006524663.5 → XP_006524726.1  A-kinase anchor protein 8 isoform X1

    Conserved Domains (1) summary
    pfam04988
    Location:362 → 522
    AKAP95; A-kinase anchoring protein 95 (AKAP95)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9DBR0.1 GenPept UniProtKB/Swiss-Prot:Q9DBR0

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