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MARCHF2 membrane associated ring-CH-type finger 2 [ Homo sapiens (human) ]

Gene ID: 51257, updated on 10-Apr-2024

Summary

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Official Symbol
MARCHF2provided by HGNC
Official Full Name
membrane associated ring-CH-type finger 2provided by HGNC
Primary source
HGNC:HGNC:28038
See related
Ensembl:ENSG00000099785 MIM:613332; AllianceGenome:HGNC:28038
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
MARCH2; RNF172; HSPC240; MARCH-II
Summary
MARCH2 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH2 reduces surface accumulation of several glycoproteins and appears to regulate early endosome-to-trans-Golgi network (TGN) trafficking (Bartee et al., 2004 [PubMed 14722266]; Nakamura et al., 2005 [PubMed 15689499]).[supplied by OMIM, Mar 2010]
Expression
Ubiquitous expression in fat (RPKM 20.9), heart (RPKM 12.9) and 25 other tissues See more
Orthologs
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Genomic context

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See MARCHF2 in Genome Data Viewer
Location:
19p13.2
Exon count:
6
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 19 NC_000019.10 (8413305..8439017)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 19 NC_060943.1 (8399779..8426935)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 19 NC_000019.9 (8478189..8503901)

Chromosome 19 - NC_000019.10Genomic Context describing neighboring genes Neighboring gene RAB11B antisense RNA 1 Neighboring gene ReSE screen-validated silencer GRCh37_chr19:8454876-8455157 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10020 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10021 Neighboring gene microRNA 4999 Neighboring gene RAB11B, member RAS oncogene family Neighboring gene Sharpr-MPRA regulatory region 2878 Neighboring gene H3K27ac hESC enhancer GRCh37_chr19:8477814-8478314 Neighboring gene Sharpr-MPRA regulatory region 12836 Neighboring gene GATA motif-containing MPRA enhancer 154 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:8488918-8489781 Neighboring gene Sharpr-MPRA regulatory region 689 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10024 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10025 Neighboring gene heterogeneous nuclear ribonucleoprotein M Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 10026 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13916 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 13917 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr19:8563393-8564079 Neighboring gene PML-RARA regulated adaptor molecule 1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Membrane-associated RING-CH (MARCH) 1 and 2 are MARCH family members that inhibit HIV-1 infection. Zhang Y, et al. J Biol Chem, 2019 Mar 8. PMID 30630952, Free PMC Article
  2. MARCH2 is upregulated in HIV-1 infection and inhibits HIV-1 production through envelope protein translocation or degradation. Zhang Y, et al. Virology, 2018 May. PMID 29573664
  3. DLG1 is an anchor for the E3 ligase MARCH2 at sites of cell-cell contact. Cao Z, et al. Cell Signal, 2008 Jan. PMID 17980554, Free PMC Article
  4. MARCH2 regulates autophagy by promoting CFTR ubiquitination and degradation and PIK3CA-AKT-MTOR signaling. Xia D, et al. Autophagy, 2016 Sep. PMID 27308891, Free PMC Article
  5. Ubiquitination and degradation of CFTR by the E3 ubiquitin ligase MARCH2 through its association with adaptor proteins CAL and STX6. Cheng J, et al. PLoS One, 2013. PMID 23818989, Free PMC Article

See all (30) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. MARCH2, a Novel Oncogene-regulated SNAIL E3 Ligase, Suppresses Triple-negative Breast Cancer Metastases.
    Title: MARCH2, a Novel Oncogene-regulated SNAIL E3 Ligase, Suppresses Triple-negative Breast Cancer Metastases.
  2. Human MARCH1, 2, and 8 block Ebola virus envelope glycoprotein cleavage via targeting furin P domain.
    Title: Human MARCH1, 2, and 8 block Ebola virus envelope glycoprotein cleavage via targeting furin P domain.
  3. MARCH2 reduced secretion of alpha1-antitrypsin and haptoglobin, and coexpression of the ubiquitination-resistant ERGIC3 variant largely restored their secretion, suggesting that MARCH2-mediated ERGIC3 ubiquitination is the major cause of the decrease in trafficking of ERGIC3-binding secretory proteins.
    Title: The E3 ubiquitin ligase MARCH2 regulates ERGIC3-dependent trafficking of secretory proteins.
  4. Membrane-associated RING-CH (MARCH) 1 and 2 are MARCH family members that inhibit HIV-1 infection
    Title: Membrane-associated RING-CH (MARCH) 1 and 2 are MARCH family members that inhibit HIV-1 infection.
  5. In this study, the authors found the expression of MARCH2 to be upregulated upon HIV-1 infection. MARCH2 inhibits the production and infection of HIV-1 through ligase activity-dependent envelope protein degradation and/or intracellular retention.
    Title: MARCH2 is upregulated in HIV-1 infection and inhibits HIV-1 production through envelope protein translocation or degradation.
  6. MARCH2 has a role in regulating autophagy by promoting CFTR ubiquitination and degradation and PIK3CA-AKT-MTOR signaling
    Title: MARCH2 regulates autophagy by promoting CFTR ubiquitination and degradation and PIK3CA-AKT-MTOR signaling.
  7. Data indicate that E3 ubiquitin ligase MARCH2 co-immunoprecipitated and co-localized with CAL and syntaxin 6 (STX6), and show the ubiquitination of CFTR by MARCH2.
    Title: Ubiquitination and degradation of CFTR by the E3 ubiquitin ligase MARCH2 through its association with adaptor proteins CAL and STX6.
  8. The MARCH2 E3 ligase activity regulates cell surface beta(2)AR expression and, consequently, its signaling.
    Title: MARCH2 promotes endocytosis and lysosomal sorting of carvedilol-bound β(2)-adrenergic receptors.
  9. MARCH2 is co-localized with DLG1 at sites of cell-cell contact.
    Title: DLG1 is an anchor for the E3 ligase MARCH2 at sites of cell-cell contact.
  10. Depletion of MARCH-II by small interfering RNA perturbed the trans-Golgi network (TGN) localization of syntaxin 6 and TGN38/46
    Title: MARCH-II is a syntaxin-6-binding protein involved in endosomal trafficking.
Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables ubiquitin protein ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ubiquitin protein ligase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables ubiquitin-protein transferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in antibacterial innate immune response ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in antiviral innate immune response ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in endocytosis IEA
Inferred from Electronic Annotation
more info
  
involved_in positive regulation of lysosomal protein catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein ubiquitination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein ubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein ubiquitination IEA
Inferred from Electronic Annotation
more info
  
involved_in protein ubiquitination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in suppression of viral release by host IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in Golgi membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasmic vesicle IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
  
located_in endoplasmic reticulum IDA
Inferred from Direct Assay
more info
 PubMed 
located_in endoplasmic reticulum membrane IEA
Inferred from Electronic Annotation
more info
  
located_in endosome membrane IEA
Inferred from Electronic Annotation
more info
  
located_in lysosomal membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
E3 ubiquitin-protein ligase MARCHF2
Names
E3 ubiquitin-protein ligase MARCH2
RING finger protein 172
RING-type E3 ubiquitin transferase MARCH2
RING-type E3 ubiquitin transferase MARCHF2
membrane associated ring finger 2
membrane-associated RING finger protein 2
membrane-associated RING-CH protein II
membrane-associated ring finger (C3HC4) 2, E3 ubiquitin protein ligase
NP_001005415.1
NP_001005416.1
NP_001356705.1
NP_001356706.1
NP_001356707.1
NP_001356708.1
NP_057580.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001005415.2 → NP_001005415.1  E3 ubiquitin-protein ligase MARCHF2 isoform 1

    See identical proteins and their annotated locations for NP_001005415.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 4. Variants 1, 2, and 4-7 all encode the longer isoform (1).
    Source sequence(s)
    AF151074, BC015910, BC032624, BI546028, CN363445
    Consensus CDS
    CCDS12202.1
    UniProtKB/Swiss-Prot
    A6NP10, Q5H785, Q8N5A3, Q96B78, Q9P0N8
    Related
    ENSP00000215555.2, ENST00000215555.7
    Conserved Domains (1) summary
    cd16808
    Location:62 → 113
    RING_CH-C4HC3_MARCH2; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING-CH2 (MARCH2)

  2. NM_001005416.2 → NP_001005416.1  E3 ubiquitin-protein ligase MARCHF2 isoform 2

    See identical proteins and their annotated locations for NP_001005416.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 4. This variant encodes isoform 2, which is shorter than isoform 1.
    Source sequence(s)
    AF151074, BC015910, BC032624, BI546028, CN363445
    Consensus CDS
    CCDS32894.1
    UniProtKB/Swiss-Prot
    Q9P0N8
    Related
    ENSP00000370423.3, ENST00000381035.8
    Conserved Domains (1) summary
    cd16808
    Location:62 → 113
    RING_CH-C4HC3_MARCH2; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING-CH2 (MARCH2)

  3. NM_001369776.1 → NP_001356705.1  E3 ubiquitin-protein ligase MARCHF2 isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) represents the longest transcript and encodes the longer isoform (1). Variants 1, 2, and 4-7 all encode the longer isoform (1).
    Source sequence(s)
    AC136469
    Consensus CDS
    CCDS12202.1
    UniProtKB/Swiss-Prot
    A6NP10, Q5H785, Q8N5A3, Q96B78, Q9P0N8
    Conserved Domains (1) summary
    cd16808
    Location:62 → 113
    RING_CH-C4HC3_MARCH2; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING-CH2 (MARCH2)

  4. NM_001369777.1 → NP_001356706.1  E3 ubiquitin-protein ligase MARCHF2 isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 4. Variants 1, 2, and 4-7 all encode the longer isoform (1).
    Source sequence(s)
    AC136469
    Consensus CDS
    CCDS12202.1
    UniProtKB/Swiss-Prot
    A6NP10, Q5H785, Q8N5A3, Q96B78, Q9P0N8
    Conserved Domains (1) summary
    cd16808
    Location:62 → 113
    RING_CH-C4HC3_MARCH2; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING-CH2 (MARCH2)

  5. NM_001369778.1 → NP_001356707.1  E3 ubiquitin-protein ligase MARCHF2 isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (6) differs in the 5' UTR compared to variant 4. Variants 1, 2, and 4-7 all encode the longer isoform (1).
    Source sequence(s)
    AC136469
    Consensus CDS
    CCDS12202.1
    UniProtKB/Swiss-Prot
    A6NP10, Q5H785, Q8N5A3, Q96B78, Q9P0N8
    Conserved Domains (1) summary
    cd16808
    Location:62 → 113
    RING_CH-C4HC3_MARCH2; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING-CH2 (MARCH2)

  6. NM_001369779.1 → NP_001356708.1  E3 ubiquitin-protein ligase MARCHF2 isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (7) differs in the 5' UTR compared to variant 4. Variants 1, 2, and 4-7 all encode the longer isoform (1).
    Source sequence(s)
    AC136469, BC032624
    Consensus CDS
    CCDS12202.1
    UniProtKB/Swiss-Prot
    A6NP10, Q5H785, Q8N5A3, Q96B78, Q9P0N8
    Related
    ENSP00000471536.1, ENST00000602117.1
    Conserved Domains (1) summary
    cd16808
    Location:62 → 113
    RING_CH-C4HC3_MARCH2; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING-CH2 (MARCH2)

  7. NM_016496.5 → NP_057580.3  E3 ubiquitin-protein ligase MARCHF2 isoform 1

    See identical proteins and their annotated locations for NP_057580.3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 4. Variants 1, 2, and 4-7 all encode the longer isoform (1).
    Source sequence(s)
    AF151074, BC015910, BC032624, BI546028, CN363445
    Consensus CDS
    CCDS12202.1
    UniProtKB/Swiss-Prot
    A6NP10, Q5H785, Q8N5A3, Q96B78, Q9P0N8
    Conserved Domains (1) summary
    cd16808
    Location:62 → 113
    RING_CH-C4HC3_MARCH2; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING-CH2 (MARCH2)

RNA

  1. NR_163145.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC136469

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000019.10 Reference GRCh38.p14 Primary Assembly

    Range
    8413305..8439017
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060943.1 Alternate T2T-CHM13v2.0

    Range
    8399779..8426935
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9P0N8.1 GenPept UniProtKB/Swiss-Prot:Q9P0N8

Gene LinkOut

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