Slc6a8 solute carrier family 6 member 8 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Slc6a8provided by RGD
Official Full Name: solute carrier family 6 member 8provided by RGD
Primary source: RGD:619711
See related: EnsemblRapid:ENSRNOG00000057620 AllianceGenome:RGD:619711
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: CRT; CT1; CHT1; CHOT1
Summary: Enables choline transmembrane transporter activity and creatine:sodium symporter activity. Involved in creatine transmembrane transport; embryonic brain development; and protein catabolic process. Located in apical plasma membrane. Is active in glutamatergic synapse and postsynaptic membrane. Biomarker of status epilepticus. Human ortholog(s) of this gene implicated in cerebral creatine deficiency syndrome 1 and intellectual disability. Orthologous to human SLC6A8 (solute carrier family 6 member 8). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Kidney (RPKM 272.7), Adrenal (RPKM 119.7) and 6 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: Xq37

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	X	NC_086039.1 (156536017..156545321)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	X	NC_051356.1 (151384675..151393979)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	X	NC_005120.4 (157129987..157139321, complement)

Chromosome X - NC_086039.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A new rat model of creatine transporter deficiency reveals behavioral disorder and altered brain metabolism.
Title: A new rat model of creatine transporter deficiency reveals behavioral disorder and altered brain metabolism.
CXCL12 contributes to cholinergic gene expression via Akt signaling pathway.
Title: CXCL12 Regulates the Cholinergic Locus and CHT1 Through Akt Signaling Pathway.
TP is expressed at high levels in human GBM tumors and shRNA knockdown of TP in U87 GBM cells results in a significant increase in cellular GCL enzymatic activity
Title: Protein τ-mediated effects on rat hippocampal choline transporters CHT1 and τ-amyloid β interactions.
The expression of CRT in cochlear tissues during development may reflect differential requirements for creatine-phosphocreatine buffering to replenish ATP consumed during energy-dependent metabolic processes.
Title: Expression and distribution of creatine transporter and creatine kinase (brain isoform) in developing and mature rat cochlear tissues.
Creatine/creatine kinase circuit may play an important role in sustaining or exacerbating acute seizure activity, but not chronic epileptic discharge.
Title: Effects of creatine and β-guanidinopropionic acid and alterations in creatine transporter and creatine kinases expression in acute seizure and chronic epilepsy models.
plasma-membrane CRT is glycosylated and has an apparent monomer molecular mass of 58 kDa; furthermore, N-linked glycosylation is neither exclusively important for the function of CRT nor for surface trafficking, but affects both processes [CRT]
Title: Effects of N-linked glycosylation on the creatine transporter.
The maturation-dependant alterations of CK and LDH isoenzyme activities and of mitochondrial oxidative capacity were paralleled by a progressive increase of creatine transporter (CrT) expression, creatine uptake kinetics and creatine content in the heart.
Title: Changes in creatine transporter function during cardiac maturation in the rat.
AMPK inhibits apical membrane CRT/Slc6a8 expression in kidney proximal tubule cells, which could be important in reducing cellular energy expenditure and unnecessary creatine reabsorption under conditions of local and whole body metabolic stress.
Title: Regulation of the creatine transporter by AMP-activated protein kinase in kidney epithelial cells.
Current results constitute the first description of the presence of a functional CRT in the axon terminal membrane that may serve to recapture the creatine released during the synapsis.
Title: Na(+)/Cl(-)/creatine transporter activity and expression in rat brain synaptosomes.
creatine transporter in the rat brain is mainly confined to neurones located principally in regions involved in motor and sensory processing and in learning, memory and limbic functions
Title: Immunohistochemical localisation of the creatine transporter in the rat brain.

Submit: New GeneRIF Correction See all GeneRIFs (16)

General gene information

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Markers

UniSTS:479272 (e-PCR)
- UniSTS:479272

Slc6a8 (e-PCR), detects polymorphism
- UniSTS:479272

DXWox37 (e-PCR)
- UniSTS:518728

D19322 (e-PCR)
- UniSTS:124805

D16S3203 (e-PCR)
- UniSTS:33197

Slc6a8 (e-PCR), detects polymorphism
- UniSTS:527552

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables choline transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables choline transmembrane transporter activity	NAS Non-traceable Author Statement more info	PubMed
enables creatine transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables creatine:sodium symporter activity	IDA Inferred from Direct Assay more info	PubMed
enables creatine:sodium symporter activity	IEA Inferred from Electronic Annotation more info
enables creatine:sodium symporter activity	ISO Inferred from Sequence Orthology more info
enables gamma-aminobutyric acid:sodium:chloride symporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in amino acid transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in choline transport	IEA Inferred from Electronic Annotation more info
involved_in creatine transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
involved_in creatine transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in creatine transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in embryonic brain development	IEP Inferred from Expression Pattern more info	PubMed
involved_in gamma-aminobutyric acid transport	IEA Inferred from Electronic Annotation more info
involved_in neurotransmitter transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of nitrogen compound transport	ISO Inferred from Sequence Orthology more info
involved_in protein catabolic process	EXP Inferred from Experiment more info	PubMed
involved_in protein catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in sodium ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	IEA Inferred from Electronic Annotation more info
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in synapse	EXP Inferred from Experiment more info	PubMed
is_active_in synapse	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: sodium- and chloride-dependent creatine transporter 1

Names: choline transporter; creatine transporter 1; solute carrier family 6 (neurotransmitter transporter), member 8; solute carrier family 6 (neurotransmitter transporter, creatine), member 8

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_017348.3 → NP_059044.1 sodium- and chloride-dependent creatine transporter 1

See identical proteins and their annotated locations for NP_059044.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

UniProtKB/Swiss-Prot

P28570

UniProtKB/TrEMBL

A0A8L2R9U9, B4F7D9

Related

ENSRNOP00000074536.2, ENSRNOT00000092123.3

Conserved Domains (1) summary

cd11509
Location:52 → 621

SLC6sbd_CT1; Na(+)- and Cl(-)-dependent creatine transporter 1; solute-binding domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086039.1 Reference GRCr8

Range

156536017..156545321

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006229611.3 → XP_006229673.1 sodium- and chloride-dependent creatine transporter 1 isoform X1

UniProtKB/TrEMBL

A0A8I5ZS61, A0A8L2R9U9

Related

ENSRNOP00000081044.1, ENSRNOT00000113585.2

Conserved Domains (1) summary

cl00456
Location:52 → 576

SLC5-6-like_sbd; Solute carrier families 5 and 6-like; solute binding domain