aPKC atypical protein kinase C [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: aPKCprovided by FlyBase
Official Full Name: atypical protein kinase Cprovided by FlyBase
Primary source: FLYBASE:FBgn0261854
Locus tag: Dmel_CG42783
See related: AllianceGenome:FB:FBgn0261854
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: a-PKC; alphaPKC; alphaPKCzeta; apkc; aPkc; aPKC zeta; aPKC-zeta; aPKCzeta; CG10261; CG30475; CG42783; dapkc; daPKC; DaPKC; Dmel\CG42783; i; l(2)k06403; PKC; pkc-3; PKCl; PKCzeta; psu
Old locus tag: CG30475; Dmel_CG10261; Dmel_CG30475
Summary: Enables myosin binding activity and protein kinase C activity. Involved in several processes, including gamete generation; morphogenesis of an epithelium; and positive regulation of plasma membrane bounded cell projection assembly. Located in apical cortex; apical plasma membrane; and apicolateral plasma membrane. Is expressed in several structures, including amnioproctodeal invagination; epithelium; extended germ band embryo; neuroblasts; and rhabdomeric photoreceptor cell. Used to study carcinoma and high grade glioma. Orthologous to human PRKCI (protein kinase C iota) and PRKCZ (protein kinase C zeta). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: 51D5-51D6; 2-73 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (14944458..14962969, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (10831963..10850474, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cooperative regulation of C1-domain membrane recruitment polarizes atypical protein kinase C.
Title: Cooperative regulation of C1-domain membrane recruitment polarizes atypical protein kinase C.
A Conserved PDZ-Binding Motif in aPKC Interacts with Par-3 and Mediates Cortical Polarity.
Title: A Conserved PDZ-Binding Motif in aPKC Interacts with Par-3 and Mediates Cortical Polarity.
A polybasic domain in aPKC mediates Par6-dependent control of membrane targeting and kinase activity.
Title: A polybasic domain in aPKC mediates Par6-dependent control of membrane targeting and kinase activity.
aPKC helps to sharpen the pattern of Miranda, by keeping it off the apical and lateral cortex after nuclear envelope breakdown.
Title: A chemical-genetics approach to study the role of atypical Protein Kinase C in Drosophila.
Therefore, phosphorylation of Miranda by aPKC and differential binding to the actomyosin network are required at distinct phases of the cell cycle to polarize fate determinant localization in neuroblasts.
Title: aPKC-mediated displacement and actomyosin-mediated retention polarize Miranda in Drosophila neuroblasts.
Here we show that PTEN mutation impacts aPKC and Lgl protein levels also in Drosophila. Moreover, we demonstrate that PI3K activation is not sufficient to trigger tumourigenesis, while aPKC promotes hyperplastic growth of the neuroepithelium and a noticeable expansion of the type II neuroblasts.
Title: Failure of the PTEN/aPKC/Lgl Axis Primes Formation of Adult Brain Tumours in Drosophila.
The authors find that the tumor suppressor Discs large (Dlg) links the Par complex component atypical Protein Kinase C (aPKC) to the essential spindle orientation factor GukHolder (GukH).
Title: Activation of Discs large by aPKC aligns the mitotic spindle to the polarity axis during asymmetric cell division.
Novel Genes Interacting with Lgl, aPKC, and Crb Cell Polarity Genes in Epithelial Tissues, is reported.
Title: A Kinome RNAi Screen in Drosophila Identifies Novel Genes Interacting with Lgl, aPKC, and Crb Cell Polarity Genes in Epithelial Tissues.
We first demonstrate that Hook2 is essential for the polarized Golgi re-orientation towards the migration front. Depletion of Hook2 results in a decrease of PAR6alpha at the centrosome during cell migration, while overexpression of Hook2 in cells induced the formation of aggresomes with the recruitment of PAR6alpha, aPKC and PAR3
Title: Hook2, a microtubule-binding protein, interacts with Par6α and controls centrosome orientation during polarized cell migration.
aPKC, spatially controlled by the Crb complex, is a key polarity molecule coordinating the generation of three distinct but interconnected cell polarities during collective migration.
Title: aPKC is a key polarity determinant in coordinating the function of three distinct cell polarities during collective migration.

Submit: New GeneRIF Correction See all GeneRIFs (51)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables PDZ domain binding	IPI Inferred from Physical Interaction more info	PubMed
enables diacylglycerol-dependent serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables myosin binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in adherens junction organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in apical protein localization	NAS Non-traceable Author Statement more info	PubMed
involved_in asymmetric neuroblast division	IGI Inferred from Genetic Interaction more info	PubMed
involved_in asymmetric protein localization involved in cell fate determination	IPI Inferred from Physical Interaction more info	PubMed
involved_in branching involved in open tracheal system development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell-cell junction assembly	NAS Non-traceable Author Statement more info	PubMed
involved_in compound eye retinal cell programmed cell death	IDA Inferred from Direct Assay more info	PubMed
involved_in epithelial cell morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in epithelial cell morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of epithelial cell apical/basal polarity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in establishment of epithelial cell planar polarity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in establishment of epithelial cell planar polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of mitotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of cell polarity	IEA Inferred from Electronic Annotation more info
involved_in establishment or maintenance of cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of epithelial cell apical/basal polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of epithelial cell apical/basal polarity	NAS Non-traceable Author Statement more info	PubMed
involved_in establishment or maintenance of neuroblast polarity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in establishment or maintenance of neuroblast polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of neuroblast polarity	NAS Non-traceable Author Statement more info	PubMed
involved_in establishment or maintenance of polarity of embryonic epithelium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of polarity of embryonic epithelium	NAS Non-traceable Author Statement more info	PubMed
involved_in establishment or maintenance of polarity of follicular epithelium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in exocyst localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germarium-derived oocyte fate determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in maintenance of cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in melanotic encapsulation of foreign target	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in memory	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in morphogenesis of a polarized epithelium	TAS Traceable Author Statement more info	PubMed
involved_in negative regulation of hippo signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of hippo signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear envelope budding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oocyte anterior/posterior axis specification	IGI Inferred from Genetic Interaction more info	PubMed
involved_in peptidyl-serine phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of filopodium assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of lamellipodium assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of neuroblast proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of smoothened signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of intracellular mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of polarized epithelial cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sensory organ development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synapse assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in terminal branching, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in zonula adherens assembly	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
part_of PAR polarity complex	IPI Inferred from Physical Interaction more info	PubMed
located_in apical cortex	IDA Inferred from Direct Assay more info	PubMed
located_in apical cortex	NAS Non-traceable Author Statement more info	PubMed
is_active_in apical junction complex	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	NAS Non-traceable Author Statement more info	PubMed
located_in apical plasma membrane	TAS Traceable Author Statement more info	PubMed
located_in apicolateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	TAS Traceable Author Statement more info	PubMed
part_of subapical complex	TAS Traceable Author Statement more info	PubMed

General protein information

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Preferred Names: atypical protein kinase C

Names: CG42783-PA; CG42783-PB; CG42783-PC; CG42783-PD; CG42783-PE; CG42783-PF; CG42783-PG; CG42783-PI; CG42783-PJ; CG42783-PK; CG42783-PL; CG42783-PM; aPKC-PA; aPKC-PB; aPKC-PC; aPKC-PD; aPKC-PE; aPKC-PF; aPKC-PG; aPKC-PI; aPKC-PJ; aPKC-PK; aPKC-PL; aPKC-PM; atypical PKC; atypical protein kinase; atypical-Protein kinase C; pschur

NP_001036541.1

EC 2.7.11.1

EC 2.7.11.13

NP_001036542.1

EC 2.7.11.1

EC 2.7.11.13

NP_001036543.1

EC 2.7.11.1

EC 2.7.11.13

NP_001036544.1

EC 2.7.11.1

EC 2.7.11.13

NP_001036545.1

EC 2.7.11.1

EC 2.7.11.13

NP_001188939.1

EC 2.7.11.1

EC 2.7.11.13

NP_001188941.1

EC 2.7.11.1

EC 2.7.11.13

NP_001260984.1

EC 2.7.11.1

EC 2.7.11.13

NP_001260985.1

EC 2.7.11.1

EC 2.7.11.13

NP_001260986.1

EC 2.7.11.1

EC 2.7.11.13

NP_001260987.1

EC 2.7.11.1

EC 2.7.11.13

NP_524892.2

EC 2.7.11.1

EC 2.7.11.13

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

14944458..14962969 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_080153.5 → NP_524892.2 atypical protein kinase C, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524892.2

Status: REVIEWED

UniProtKB/Swiss-Prot

A1Z9X0, Q0E971, Q0E972, Q0E973, Q0E974, Q8MT38, Q9GSZ3

Related

FBpp0292491

Conserved Domains (4) summary

cd06404
Location:31 → 113

PB1_aPKC; PB1 domain is an essential modular domain of the atypical protein kinase C (aPKC) which in complex with Par6 and Par3 proteins is crucial for establishment of apical-basal polarity of animal cells. PB1 domain is a modular domain mediating specific ...

smart00220
Location:264 → 532

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05588
Location:268 → 595

STKc_aPKC; Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C

pfam00130
Location:146 → 198

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
NM_001043080.3 → NP_001036545.1 atypical protein kinase C, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001036545.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A1Z9X0

Conserved Domains (4) summary

cd06404
Location:31 → 113

PB1_aPKC; PB1 domain is an essential modular domain of the atypical protein kinase C (aPKC) which in complex with Par6 and Par3 proteins is crucial for establishment of apical-basal polarity of animal cells. PB1 domain is a modular domain mediating specific ...

smart00220
Location:264 → 532

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05588
Location:268 → 597

STKc_aPKC; Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C

pfam00130
Location:146 → 198

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
NM_001274055.1 → NP_001260984.1 atypical protein kinase C, isoform J [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260984.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KG15

Conserved Domains (3) summary

smart00220
Location:616 → 884

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05588
Location:620 → 947

STKc_aPKC; Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C

pfam00130
Location:498 → 550

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
NM_001274056.2 → NP_001260985.1 atypical protein kinase C, isoform K [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260985.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KFQ6

Conserved Domains (3) summary

smart00220
Location:177 → 445

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05588
Location:181 → 508

STKc_aPKC; Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C

pfam00130
Location:59 → 111

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
NM_001274058.2 → NP_001260987.1 atypical protein kinase C, isoform M [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260987.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KEQ8

Conserved Domains (3) summary

smart00220
Location:161 → 429

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05588
Location:165 → 492

STKc_aPKC; Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C

pfam00130
Location:43 → 95

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
NM_001274057.2 → NP_001260986.1 atypical protein kinase C, isoform L [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260986.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KEV2

Conserved Domains (3) summary

smart00220
Location:161 → 429

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05588
Location:165 → 492

STKc_aPKC; Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C

pfam00130
Location:43 → 95

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
NM_001043077.3 → NP_001036542.1 atypical protein kinase C, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001036542.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A1Z9X0

Conserved Domains (2) summary

cd05588
Location:165 → 492

STKc_aPKC; Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C

cd20794
Location:41 → 95

C1_aPKC; protein kinase C conserved region 1 (C1 domain) found in the atypical protein kinase C (aPKC) family
NM_001043076.3 → NP_001036541.1 atypical protein kinase C, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001036541.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A1Z9X0, Q0E971, Q0E972, Q0E973, Q0E974, Q8MT38, Q9GSZ3

Conserved Domains (4) summary

cd06404
Location:31 → 113

PB1_aPKC; PB1 domain is an essential modular domain of the atypical protein kinase C (aPKC) which in complex with Par6 and Par3 proteins is crucial for establishment of apical-basal polarity of animal cells. PB1 domain is a modular domain mediating specific ...

smart00220
Location:264 → 532

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05588
Location:268 → 595

STKc_aPKC; Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C

pfam00130
Location:146 → 198

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
NM_001202010.4 → NP_001188939.1 atypical protein kinase C, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001188939.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4JD78

Conserved Domains (3) summary

smart00220
Location:609 → 877

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05588
Location:613 → 940

STKc_aPKC; Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C

pfam00130
Location:491 → 543

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
NM_001043078.2 → NP_001036543.1 atypical protein kinase C, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001036543.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A1Z9X0

Conserved Domains (2) summary

cd05588
Location:186 → 513

STKc_aPKC; Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C

cd20794
Location:62 → 116

C1_aPKC; protein kinase C conserved region 1 (C1 domain) found in the atypical protein kinase C (aPKC) family
NM_001043079.2 → NP_001036544.1 atypical protein kinase C, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001036544.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A1Z9X0

Conserved Domains (2) summary

cd05588
Location:186 → 515

STKc_aPKC; Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C

cd20794
Location:62 → 116

C1_aPKC; protein kinase C conserved region 1 (C1 domain) found in the atypical protein kinase C (aPKC) family
NM_001202012.2 → NP_001188941.1 atypical protein kinase C, isoform I [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001188941.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4JD26

Conserved Domains (3) summary

smart00220
Location:182 → 450

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05588
Location:186 → 498

STKc_aPKC; Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C

pfam00130
Location:64 → 116

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

Nucleotide		Protein
Heading	Accession and Version	Protein

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
A0A0B4JD26		A0A0B4JD26
A0A0B4JD78		A0A0B4JD78
A0A0B4KEQ8		A0A0B4KEQ8
A0A0B4KEV2		A0A0B4KEV2
A0A0B4KFQ6		A0A0B4KFQ6
A0A0B4KG15		A0A0B4KG15
A1Z9X0.1		A1Z9X0