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comt comatose [ Drosophila melanogaster (fruit fly) ]

Gene ID: 47091, updated on 10-May-2024

Summary

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Official Symbol
comtprovided by FlyBase
Official Full Name
comatoseprovided by FlyBase
Primary source
FLYBASE:FBgn0000346
Locus tag
Dmel_CG1618
See related
AllianceGenome:FB:FBgn0000346
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
CG 1618; CG1618; com; Comt; Dmel\CG1618; DmNSF; dNSF; dNSF-1; dnsf1; dNsf1; dNSF1; Nsf; NSF; NSF 1; NSF-1; Nsf1; NSF1
Summary
Predicted to enable ATP hydrolysis activity. Involved in several processes, including Golgi to lysosome transport; chemical synaptic transmission; and modulation of chemical synaptic transmission. Located in cytoplasm; neuromuscular junction of somatic muscle; and terminal bouton. Colocalizes with synaptic vesicle. Is expressed in several structures, including adult optic lobe; dorsal sensory complex proper primordium; embryonic/larval nervous system; extended germ band embryo; and presumptive embryonic/larval nervous system. Used to study Parkinson's disease. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy. Orthologous to human NSF (N-ethylmaleimide sensitive factor, vesicle fusing ATPase). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs
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Genomic context

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See comt in Genome Data Viewer
Location:
11E8-11E8; 1-42 cM
Exon count:
7
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) X NC_004354.4 (13259021..13262488)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) X NC_004354.3 (13153054..13156521)

Chromosome X - NC_004354.4Genomic Context describing neighboring genes Neighboring gene multiple edematous wings Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene Histone deacetylase 4 Neighboring gene uncharacterized protein

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. SNAPs and NSF: general members of the fusion apparatus. Whiteheart SW, et al. Trends Cell Biol, 1995 Feb. PMID 14731414
  2. A Drosophila NSF mutant. Pallanck L, et al. Nature, 1995 Jul 6. PMID 7596428
  3. The ubiquitin ligase Ariadne-1 regulates neurotransmitter release via ubiquitination of NSF. Ramírez J, et al. J Biol Chem, 2021 Jan-Jun. PMID 33581113, Free PMC Article
  4. Conditional mutations in SERCA, the Sarco-endoplasmic reticulum Ca2+-ATPase, alter heart rate and rhythmicity in Drosophila. Sanyal S, et al. J Comp Physiol B, 2006 Mar. PMID 16320060
  5. Courtship and other behaviors affected by a heat-sensitive, molecularly novel mutation in the cacophony calcium-channel gene of Drosophila. Chan B, et al. Genetics, 2002 Sep. PMID 12242229, Free PMC Article

See all (117) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The ubiquitin ligase Ariadne-1 regulates neurotransmitter release via ubiquitination of NSF.
    Title: The ubiquitin ligase Ariadne-1 regulates neurotransmitter release via ubiquitination of NSF.
  2. This study illustrated the comatose mutations in protein trafficking and synaptic vesicles function.
    Title: Genetic modifiers of comatose mutations in Drosophila: insights into neuronal NSF (N-ethylmaleimide-sensitive fusion factor) functions.
  3. Data show that NSF and SNAP exhibit activity-dependent binding to each other within living presynaptic terminals as well as distinctive interactions and mobilities.
    Title: Activity-dependent interactions of NSF and SNAP at living synapses.
  4. Results establish in vivo contributions of SNAP-25 and NSF to synaptic vesicle trafficking and define molecular mechanisms determining conserved functional properties of short-term depression.
    Title: Molecular mechanisms determining conserved properties of short-term synaptic depression revealed in NSF and SNAP-25 conditional mutants.
  5. Mutants Of NSF have been engineered that define key residues involved in two essential, but mechanistically distinct, biochemical functions of NSF: SNAP binding and SNAP-dependent ATPase stimulation.
    Title: Analysis of NSF mutants reveals residues involved in SNAP binding and ATPase stimulation.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity ISS
Inferred from Sequence or Structural Similarity
more info
  
Process Evidence Code Pubs
involved_in Golgi organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in Golgi to lysosome transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in Golgi to plasma membrane protein transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in SNARE complex disassembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in SNARE complex disassembly IEA
Inferred from Electronic Annotation
more info
  
involved_in autophagy IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in intra-Golgi vesicle-mediated transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in neuromuscular synaptic transmission IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in neuron cellular homeostasis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in neurotransmitter secretion NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in regulation of neuromuscular synaptic transmission IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in regulation of short-term neuronal synaptic plasticity IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in synaptic vesicle cycle IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in synaptic vesicle cycle IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in synaptic vesicle cycle IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in synaptic vesicle fusion to presynaptic active zone membrane IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in synaptic vesicle priming NAS
Non-traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
is_active_in Golgi stack IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm NAS
Non-traceable Author Statement
more info
 PubMed 
is_active_in neuromuscular junction IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in neuromuscular junction IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in neuromuscular junction of somatic muscle IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in presynaptic cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in presynaptic cytosol IMP
Inferred from Mutant Phenotype
more info
 PubMed 
colocalizes_with synaptic vesicle IDA
Inferred from Direct Assay
more info
 PubMed 
located_in terminal bouton IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
comatose
Names
CG1618-PA
CG1618-PB
N-Ethylmaleimide-sensitive factor
N-ethyl-maleimide sensitive fusion protein
N-ethylmaleimide-sensitive factor 1
N-ethylmaleimide-sensitive fusion protein
NEM-sensitive fusion protein
comt-PA
comt-PB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_004354.4 Reference assembly

    Range
    13259021..13262488
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_080138.4 → NP_524877.1  comatose, isoform A [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_524877.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    P46461, Q9VYF4
    UniProtKB/TrEMBL
    M9PH10
    Related
    FBpp0073585
    Conserved Domains (4) summary
    smart01072
    Location:111 → 158
    CDC48_2; Cell division protein 48 (CDC48) domain 2
    smart01073
    Location:5 → 83
    CDC48_N; Cell division protein 48 (CDC48) N-terminal domain
    COG0464
    Location:242 → 699
    SpoVK; AAA+-type ATPase, SpoVK/Ycf46/Vps4 family [Cell wall/membrane/envelope biogenesis, Cell cycle control, cell division, chromosome partitioning, Signal transduction mechanisms]
    pfam00004
    Location:256 → 397
    AAA; ATPase family associated with various cellular activities (AAA)

  2. NM_001272577.2 → NP_001259506.1  comatose, isoform B [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001259506.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    P46461, Q9VYF4
    UniProtKB/TrEMBL
    M9PH10
    Conserved Domains (4) summary
    smart01072
    Location:111 → 158
    CDC48_2; Cell division protein 48 (CDC48) domain 2
    smart01073
    Location:5 → 83
    CDC48_N; Cell division protein 48 (CDC48) N-terminal domain
    COG0464
    Location:242 → 699
    SpoVK; AAA+-type ATPase, SpoVK/Ycf46/Vps4 family [Cell wall/membrane/envelope biogenesis, Cell cycle control, cell division, chromosome partitioning, Signal transduction mechanisms]
    pfam00004
    Location:256 → 397
    AAA; ATPase family associated with various cellular activities (AAA)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
M9PH10 M9PH10
P46461.1 P46461

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