spir spire [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: spirprovided by FlyBase
Official Full Name: spireprovided by FlyBase
Primary source: FLYBASE:FBgn0003475
Locus tag: Dmel_CG10076
See related: AllianceGenome:FB:FBgn0003475
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 38C.34; 38C.37; CG10076; CG18621; Dmel\CG10076; l(2)08327; NP2788; p150-Spir; p150Spir; Spir; Spire
Summary: Enables microtubule binding activity. Involved in actin filament organization; oogenesis; and regulation of cytoskeleton organization. Located in cell cortex. Is expressed in several structures, including germline cell; gland; gut section; nephrocyte; and sensory nervous system primordium. Orthologous to human SPIRE1 (spire type actin nucleation factor 1) and SPIRE2 (spire type actin nucleation factor 2). [provided by Alliance of Genome Resources, Nov 2024]
Orthologs: all
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Genomic context

Location:: 38C5-38C5; 2-54 cM

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (20311219..20348386)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (20311219..20348386)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Spire stimulates nucleation by Cappuccino and binds both ends of actin filaments.
Title: Spire stimulates nucleation by Cappuccino and binds both ends of actin filaments.
this used Drosophila Spire (Spir) as a model system to investigate both how tandem WH2 domains can nucleate actin and what differentiates nucleating WH2-containing proteins from their non-nucleating counterparts.
Title: Filament assembly by Spire: key residues and concerted actin binding.
Lola-Spir pathway is crucial for the spatial arrangement of branches within dendritic trees.
Title: Dendrite architecture organized by transcriptional control of the F-actin nucleator Spire.
Direct interaction between two actin nucleators (Spir and Capu) is required in Drosophila oogenesis.
Title: Direct interaction between two actin nucleators is required in Drosophila oogenesis.
Data conclude that Spir plays a crucial role in controlling dorsal vessel formation and has a function in cell division during heart tube morphogenesis.
Title: Spire, an actin nucleation factor, regulates cell division during Drosophila heart development.
Multiple forms of Spire-actin complexes and their functional consequences.
Title: Multiple forms of Spire-actin complexes and their functional consequences.
Data suggest that the molecular and structural mechanism of actin polymerization by Spire should apply to other actin-binding proteins that contain WH2 domains in tandem.
Title: Molecular architecture of the Spire-actin nucleus and its implication for actin filament assembly.
Lola is a negative regulator of spire, derepression of spire is an important contributor to the axon stalling phenotype of embryonic motor axons in lola mutants.
Title: A genome-wide analysis reveals that the Drosophila transcription factor Lola promotes axon growth in part by suppressing expression of the actin nucleation factor Spire.
Crystal structures of the complexes between the actin-binding WH2 repeats of the Spire protein and actin were determined for the Spire single domain D, the double (SpirCD), triple (SpirBCD), quadruple (SpirABCD) domains, and an artificial Spire (SpirDDD).
Title: Structures of actin-bound Wiskott-Aldrich syndrome protein homology 2 (WH2) domains of Spire and the implication for filament nucleation.
Spire overexpression cannot rescue capu mutants, but prevents actin mesh disassembly at stage 10B and blocks late cytoplasmic streaming.
Title: Capu and Spire assemble a cytoplasmic actin mesh that maintains microtubule organization in the Drosophila oocyte.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin binding	TAS Traceable Author Statement more info	PubMed
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Golgi vesicle transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin cytoskeleton organization	TAS Traceable Author Statement more info	PubMed
involved_in actin filament network formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin filament network formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament polymerization	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin filament-based process	IDA Inferred from Direct Assay more info	PubMed
involved_in actin nucleation	IDA Inferred from Direct Assay more info	PubMed
involved_in actin nucleation	IEA Inferred from Electronic Annotation more info
involved_in chorion-containing eggshell formation	HMP more info	PubMed
involved_in cleavage furrow formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment of meiotic spindle localization	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in oocyte karyosome formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	TAS Traceable Author Statement more info	PubMed
involved_in polar body extrusion after meiotic divisions	IBA Inferred from Biological aspect of Ancestor more info
involved_in pole plasm RNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pole plasm assembly	HMP more info	PubMed
involved_in pole plasm assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pole plasm assembly	NAS Non-traceable Author Statement more info	PubMed
involved_in pole plasm assembly	TAS Traceable Author Statement more info	PubMed
involved_in pole plasm oskar mRNA localization	TAS Traceable Author Statement more info	PubMed
involved_in positive regulation of mitochondrial fission	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of cytoskeleton organization	IDA Inferred from Direct Assay more info	PubMed
involved_in vesicle-mediated transport	IEA Inferred from Electronic Annotation more info
involved_in vesicle-mediated transport	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
is_active_in cell cortex	IBA Inferred from Biological aspect of Ancestor more info
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasmic vesicle membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial outer membrane	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: spire

Names: CG10076-PA; CG10076-PB; CG10076-PC; CG10076-PD; CG10076-PE; CG10076-PF; CG10076-PG; CG10076-PH; CG10076-PI; CG10076-PJ; spir-PA; spir-PB; spir-PC; spir-PD; spir-PE; spir-PF; spir-PG; spir-PH; spir-PI; spir-PJ

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

20311219..20348386

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001299171.1 → NP_001286100.1 spire, isoform J [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001286100.1

Status: REVIEWED

UniProtKB/TrEMBL

M9NFB6

Conserved Domains (1) summary

cl21453
Location:90 → 327

PKc_like; Protein Kinases, catalytic domain
NM_165323.3 → NP_724254.1 spire, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_724254.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q5U0Z8, Q8INV3, Q8INV4, Q8INV5, Q8SXP3, Q8T8P8, Q9U1K1, Q9U4F0, Q9U4F1, Q9VIN3, Q9VIN4

Related

FBpp0080884

Conserved Domains (2) summary

smart00750
Location:90 → 327

KIND; kinase non-catalytic C-lobe domain

cd15748
Location:739 → 774

FYVE_SPIR; FYVE-related domain found in Spir proteins, Spire1 and Spire2
NM_080115.3 → NP_524854.2 spire, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524854.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9U1K1

Related

FBpp0080885

Conserved Domains (2) summary

smart00750
Location:90 → 327

KIND; kinase non-catalytic C-lobe domain

cd15748
Location:710 → 745

FYVE_SPIR; FYVE-related domain found in Spir proteins, Spire1 and Spire2
NM_001169549.1 → NP_001163020.1 spire, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001163020.1

Status: REVIEWED

UniProtKB/TrEMBL

E1JHM3

Conserved Domains (2) summary

smart00750
Location:90 → 327

KIND; kinase non-catalytic C-lobe domain

cd15748
Location:709 → 744

FYVE_SPIR; FYVE-related domain found in Spir proteins, Spire1 and Spire2
NM_001259172.2 → NP_001246101.1 spire, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246101.1

Status: REVIEWED

UniProtKB/TrEMBL

M9NDT9

Conserved Domains (2) summary

smart00750
Location:90 → 327

KIND; kinase non-catalytic C-lobe domain

cd15748
Location:737 → 772

FYVE_SPIR; FYVE-related domain found in Spir proteins, Spire1 and Spire2
NM_001259173.1 → NP_001246102.1 spire, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246102.1

Status: REVIEWED

UniProtKB/TrEMBL

M9NFB6

Conserved Domains (1) summary

cl21453
Location:90 → 327

PKc_like; Protein Kinases, catalytic domain
NM_165324.2 → NP_724255.1 spire, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_724255.1

Status: REVIEWED

UniProtKB/TrEMBL

D4G7C9

Related

FBpp0080886

Conserved Domains (1) summary

cl21453
Location:90 → 327

PKc_like; Protein Kinases, catalytic domain
NM_001299170.1 → NP_001286099.1 spire, isoform I [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001286099.1

Status: REVIEWED

UniProtKB/TrEMBL

X2J709

Conserved Domains (1) summary

smart00750
Location:90 → 327

KIND; kinase non-catalytic C-lobe domain
NM_001259174.1 → NP_001246103.1 spire, isoform H [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246103.1

Status: REVIEWED

UniProtKB/TrEMBL

M9NCY7

Conserved Domains (1) summary

smart00750
Location:90 → 327

KIND; kinase non-catalytic C-lobe domain
NM_165325.3 → NP_724256.2 spire, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_724256.2

Status: REVIEWED

UniProtKB/TrEMBL

B5RIQ1

Related

FBpp0080887

Conserved Domains (1) summary

cd15748
Location:344 → 379

FYVE_SPIR; FYVE-related domain found in Spir proteins, Spire1 and Spire2